Dopamin ve Ürik Asitin Birinin Varlığında Diğerinin Kare Dalga Voltametrisi ile Tayini

Bu çalışmada çeşitli destek elektrolit ortamlarında DA ve ÜA' nın yükseltgenme davranışları incelenmiş, pH 2,5 H2SO4 ve pH 7,2 fosfat tamponunun uygun olduğuna karar verilmiştir. pH 2,5 H2SO4 ortamında DA ve ÜA' nın sırasıyla +0,480 V, +0,620 V' da ki, fosfat tamponunda da sırasıyla +0,140 V, +0,270 V' da ki yükseltgenme pikleri kullanılmıştır. Her iki ortamda farklı [DA] / [ÜA] oranları çalışılmıştır. Sentetik numune için, pH 2,5 H2SO4 ortamında DA ve ÜA için % hata sırasıyla -0,41 ve 0,27, pH 7,2 fosfat tamponu ortamında aynı numune için % hata sırasıyla -0,61 ve -0,14 olarak bulunmuştur. pH 2,5 H2SO4 ortamında DA ve ÜA için gözlenebilme sınırı (LOD) sırasıyla 2,16 Ã 10-8 M ve 4,14 Ã 10-8 M, tayin sınırı (LOQ) ise 6,48 Ã 10-8 M ve 1,24 Ã 10-7 M olarak bulunmuştur. pH 7,2 fosfat tamponu ortamında DA ve ÜA için gözlenebilme sınırı (LOD) sırasıyla 2,28 Ã 10-8 M ve 2,13 Ã 10-8 M, tayin sınırı (LOQ) 6,84 Ã 10-8 M ve 6,39 Ã 10-8 M olarak bulunmuştur. Bu yönteme çeşitli anyon ve katyonların

Anahtar Kelimeler:

Kare dalga voltametrisi, Tayin, Dopamin, Ürik asit.

The Determination of Dopamine and Uric Acid with the Presence of Other Using Square Wave Voltammetry

Initially the electrochemical behavior of dopamine (DA) and uric acid (UA) in different supporting electrolytes was investigated. It was found that the most suitable supporting electrolytes were pH 2.5 H2SO4 and pH 7.2 phosphate buffer. The analysis of DA and UA in acidic medium was carried out by the use of their oxidation peaks observed at +0.480 V and +0.620 V, respectively. The analysis of the phosphate buffer medium were carried out by the use of the oxidation peaks of DA and UA appeared at +0.140 V and +0.270 V, respectively. The studies carried out with different [DA] / [UA] ratios gave acceptable results for both media. The synthetic sample was analyzed. The error was -0.41 % for DA and 0.27 % for UA in acidic media and -0.61 % for DA and -0.14 % for UA in basic media. The limits of detection (LOD) were found as 2.16 Ã 10-8 M and 4.14 Ã 10-8 M in acidic media, 2.28 Ã 10-8 M and 2.13 Ã 10-8 M in basic media for DA and UA, respectively.

Keywords:

Square wave voltametry, Determination, Dopamine, Uric acid.,

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