Özge Tuğçe PAŞAOĞLU, Ayşe GÜNDOĞDU ÇAKIR, Gülnur KAPLANOĞLU TAKE, Onur AKARCA, Özgür ÖCAL, Erkut Baha BULDUK, Aydın PAŞAOĞLU

Rat Siyatik Siniri Iskemi-Reperfüzyon Hasarında Resveratrolün Koruyucu Etkileri

Amaç: Bu çalışmanın amacı sıçan siyatik sinirinde resveratrolün iskemireperfüzyon (I / R) hasarı üzerine etkilerini belirlemektir. Yöntem: Ketamin (50 mg / kg-im) ile anestezi uygulanmış 18 adet Wistar sıçanı kullandık. Sham grubunda herhangi bir ilaç vermeden sadece cerrahi uyguladık. İskemi grubunda 5 dakika aralıklarla I / R hasarı oluşturduk ve medikal tedavi olarak sadece salin verdik. Resveratrol grubunda da aynı I / R hasarını oluşturduk, ancak oral yolla işlemden önce resveratrol verdik. Ratlar sakrifiye edildikten sonra kan ve sinir doku örnekleri aldık. Hem doku hem de kandaki malondialdehit (MDA), nitrik oksit (NO) ve toplam antioksidan kapasiteleri (TAC) ile hasarı değerlendirdik. Bulgular: Antioksidan belirteçlerin ve doku örneklerinin serum düzeylerinin değerlendirilmesi, iskemi-reperfüzyon hasarında resveratrolün istatistiksel olarak anlamlı etkilerini ortaya koymuştur. Sonuç: Bu çalışma lipid peroksidasyonunun bir önleyicisi olarak resveratrolün antioksidan etkisini ortaya koymuştur.

Protective Effects of Resveratrol in a Rat Model of Ischemia-Reperfusion Injury of Sciatic Nerve

Objective: The aim of this study was to determine the effects of resveratrol on ischemia- reperfusion (I/R) injury in the rats sciatic nerve. Methods: We used 18 Wistar rats anesthetized with ketamine (50 mg/kg-im). In the sham group, we only performed the surgery and not administer any medication. In the ischemia group, we generated I/R injury in 5 minute intervals with medical treatment of saline only. In the resveratrol group, we brought about the same I/R injury but gave resveratrol before the surgery by per oral. After sacrifice, we studied blood and nerve tissue samples. We evaluated the damage with malondialdehyde (MDA), nitric oxide (NO), and total antioxidant capacity (TAC), both in tissue and blood. Results: Evaluation of serum levels of antioxidant markers and tissue samples demonstrated statistically significant effects of resveratrol in ischemiareperfusion injury. Conclusion: This study revealed the antioxidant effect of resveratrol as an inhibitor of lipid peroxidation.

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