Purpose: To assess the effect of laser prostatectomy (LP) procedure on serum prostate-specific antigen (PSA) levels and free/total PSA (f/t). To our knowledge, this is the first study on this subject with respect to free/total PSA (f/t) levels. Patients and Methods: The serum PSA levels and f/t ratio were determined in 30 patients with benign prostatic hyperplasia (BPH) l day before and l, 7,30 days after LP. AH patients undenwent preoperative evaluation with routine blood tests, serum PSA levels (free and total), IPSS symptom questionnaire, intravenous pyelography (IVP), uroflovmetry, post void residual urine measurements, and transrectal Ultrasonography (TRUS). Results: Total and free PSA levels shoved a statistically significant (p