Rastgele çoğaltılmış polimorfik DNA yöntemiyle kanatlı etlerinde tür tayini

Bu çalışmada, 9 adet kanatlı etinde (tavuk, hindi, martı, devekuşu, ördek, kaz, bıldırcın, keklik ve karga) Rastgele Çoğaltılmış Polimorfik DNA yöntemi kullanılarak tür tayini yapıldı.Çalışmada, Guanin-Sitozin oranları %50 ile %80 arasında değişen, 12 adet 10 bazlık primer kullanıldı. Kanatlı türleri, Rastgele çoğaltılmış polimorfik DNA profilleri karşılaştırılarak ayırt edildi. Sonuç olarak, kullanılan bazı primerlerin kanatlı etlerinde tür tayini için uygun olduğu bazılarının ise uygun olmadığı tespit edildi. Çalışmanın sonuçları, Rastgele çoğaltılmış polimorfik DNA yöntemiyle kanatlı etlerinde tür tayini için uygun primerlerin kullanılması gerektiğini gösterdi.

Identification of poultry meat species by random amplified polymorphic DNA (RAPD) technique

In this study, meats of 9 poultry species (chicken, turkey, gull, ostrich, duck, goose, quail, partridge, and crow) were identified by random amplified polymorphic DNA (RAPD) method using 12 different10 bp-nucleotjde primers with guanine and cytosine (GC) contents, ranging from 50-80%. In general, the species were differentiated by comparing RAPD fingerprinting of the species.However, some primers used were not suitable for identification of poultry meats while the some, primers were suitable. Therefore, the results indicated that identification of poultry meat species by RAPD technique requires use of suitable primers.

PDF

___

1. İlhak İ, Arslan A. Random amplified polymorphic DNA (RAPD) yöntemiyle sığır, koyun, keçi ve yabani domuz etinin ayırt edilmesi. F.Ü. Sağlık Bilimleri Dergisi 2003; 17: 59-63. 2. Kang'ethe EK, Jones SJ, Patterson RLS. Identification of the species origin of fresh meat using an ELISA procedure. Meat Sci 1982; 7: 229-240.
3. Allsup TN. A comparison of the ağar gel.immuno-diffusion (AGID) and counter-immunoelectrophoresis(CIE) tests for species identification of imported red meat and offal. Meat Sci 1987; 20: 119-128.
4. Wintero AK, Thomsen PD, Davies W. A comparison of DNA- hybridization, immunodiffusion, countercurrent immunoelectrophoresis and isoelectric focusing for detecting the admixture of pork to beef. Meat Sci 1990; 27: 75-85.
5. Ebbehoj FK, Thomsen DP. Species differentiation of heated meat products by DNA hybridization. Meat Sci 1991; 30: 221-234.
6. Hird H, Goodier R, Hill M. Rapid detection of chicken and turkey in heated meat products using the polymerase chain reaction followed by amplicon visualisation with vistra green. Meat Sci 2003; 65: 1117-1123.
7. Lee CJ, Chang GJ. Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) fingerprints in forensic species identification. Forensic Sci Int 1994; 67: 103-107.
8. Koh MC, Lim CH, Chua SB, Chew ST, Phang STW. Random Amplified Polymorphic DNA ( RAPD ) fingerprints for identification of red meat animal species. Meat Sci 1998; 48: 275-285.
9. Zhang G, Zheng M, Zhou Z, Ouyang H, Lu Q. Establishment and application of a polymerase chain reaction for the identification of beef. Meat Sci 1999; 51: 233-236.
10. Partis L, Croan D, Guo Z, Clark R, Coldham T, MurbyJ. Evaluation of a DNA fingerprinting method for determining the species origin of meats. Meat Sci 2000; 54: 369-376.'
11. Meyer R, Höfelein C, Luthy J, Candrian U. Polymerase chain reaction-restriction fragment length polymorphism analysis: A simple method for species identification in food. J AOAÇ Int 1995; 78: 1542-1551
12. Martinez I, Yman Ml. Species identification in meat products by RÂPD analysis. Food Res Int 1998; 31: 459-466.
13. Saez R, Sanz ,Y, Toldra F. PCR-based fingerprinting techniques for rapid detection of animal species in meat products. Meat Sci 2004; 66; 659-665.
14. Arslan A, İlhak I, Calicioglu M, Karahan M. Identification of meats using random amplified polymorphic DNA (RAPD) technique. J Muscle Foods 2005; 16: 37-45.
15. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 19Ö0; 18:6531-6535.
16. Matsunaga T, Chikuni K.Tanabe R, Muroya S, Shibata K, Yamada J, Shinmura YA. Quick and simple method for the identification of meat species and meat products by PCR assay. Meat Sci 1999; 51: 143-148.
17. Welsh J, McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 1990; 18: 7213-7218.

Fırat Üniversitesi Sağlık Bilimleri Dergisi-Cover

ISSN: 1012-0173
Yayın Aralığı: Yıllık
Yayıncı: -

Arşiv

Sayıdaki Diğer Makaleler

Oestrosis ve coenurosis'e bağlı olarak epileptoit nöbet gösteren bir koyun sürüsünde klinik ve patolojik gözlemler

Mustafa İSSİ, Yusuf Gül, Songül ÖZER

Rastgele çoğaltılmış polimorfik DNA yöntemiyle kanatlı etlerinde tür tayini

Ali ARSLAN, O. İrfan İLHAK

Süt ineklerinde yatalak hal (Recumbent Cow, festligende Küh)

Mustafa İSSİ, Yusuf GÜL, Hüseyin DEVECİ

Köpeklerde deneysel geniş defektli karın fıtıklarının sağaltımında otojen fascia lata kullanımı

İhsan YAMAN, Ali. Said DURMUŞ, Mehmet Cengiz HAN

morphological and morphometric approach to the bones of the wings in the long-legged buzzard (Buteo Rufinus)

İbrahim KÜRTÜL, Ömer ATALAR, Derviş ÖZDEMİR

The different between methods and determining of metabolisable energy levels with enzyme and gas technigues in concentrate feeds

İbrahim Halil ÇERÇİ, Mehmet Ali AZMAN, Tatlı Pınar SEVEN