Elif TAŞLIDERE, MELTEM KURUŞ, Alper KAZANCI, Ali OTLU

Sıçanlarda özefagus ve midede yaşa bağlı değişimlerin histomorfolojik açıdan incelenmesi

Amaç: Bu çalışmada sıçan özefagus ve midelerinde yaşa bağlı olarak gelişen morfolojik değişikliklerin ışık mikroskobik açıdan incelenmesi amaç- landı. Gereç ve Yöntem: Çalışmamızda 21 adet dişi Spraque-Dawley sıçan kullanıldı. Sıçanlar, Grup 1: 21 günlük sıçanlar, Grup 2: 2 aylık sıçanlar ve Grup 3: 19 aylık sıçanlar olmak üzere 3 gruba ayrıldı. Deney sonunda çıkartılan özefagus ve mide dokuları rutin histolojik doku takibinden sonra parafine gömüldü. Kesitler Hematoksilen-eozin ile boyanıp ışık mikroskobunda incelendi. Bulgular: Çalışmamızda özefagus epitel kalınlığı Grup 1’de ortalama 31.85±5.7 μm, Grup 2’de 40.95±6.7 μm, Grup 3’de 44.68±9,2 μm olarak bulundu. Tunika muskularis kalınlığı Grup 1’de ortalama 128.9±23.1 μm, Grup 2’de 207.8±38.5 μm ve Grup 3’de 256.07±35.52 μm olarak tespit edildi. Sıçanlarda özefagusa ait epitel ve tunika muskularis kalınlığı ölçüldüğünde yaşlanmaya bağlı olarak kalınlığın anlamlı derecede arttığı gözlendi (p

Histomorphological examination of age-related change in rat esophagus and stomach

Objective: In this study, it was aimed that age-related morphological changes in rat esophagus and stomach was investigated with light microscopy. Materials and Methods: In this study, 21 female Sprage-Dawley rats were used. Rats were divided into three groups as follows. Group1:21 days old, Group2:2 month old and Group3:19 month old. At the end of the examination esophagus and stomach tissues were removed.The sections stained wiht hematoxylin-eosin and evaluated light microscopically. Results: The mean thickness of esophagus epithelium was measured. It was 31.85±5.7 μm in Group1, 40.95±6.7 μm in Group2 and 44.68±9.2 μm in Group3. The mean thickness of tunica muscularis in esophagus measured. It was 128.9±23.1 μm in Group 1, 207.8±38,5 μm in Group2 and 256.07±35.52 in Group3. We observed that the thickness of esophagus epithelium and tunica muscularis were significantly increased by age in all groups (p<0.05). Likewise, the mean thickness of tunica mucosa in stomach was measured. It was 67.87±8.25 μm in Group1, 127.76±21.8 μm in Group2 and 181.23±35.1 μm in Group3. The mean thickness of tunika muscularis in stomach was measured. It was 56.65±15.9 μm in Group1, 125.68±21.36μm in Group2, 174.07±22.5 μm in Group3. We observed that the thickness of tunica mucosa and tunica muscularis were significantly increased by age in all groups (p<0.05). Conclusion: The morfology of esophagus and stomach in rats were observed to change with increasing age. We believe that obtained in this study as age-related changes in rat esophagus and stomach may shed light on other morphological studies and that will contribute to the literature.

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Williams PL, Bannister LH, Berry MM, et al. Embriyology and Development In: Collins P. Grays Anatomy. Churcill Livingstone Inc, New York 1995: 181-85.
Moore KL, Persaud, T. V.N Klinik yönleri ile insan embriyolojisi. In: Yıldırım M, Okar İ, Dalçık H. ed. 1. Baskı, İstanbul: Nobel Matbaacılık, 2002: 212-13.
Ovalle WK, Nahirney PC. Netter Temel Histoloji. In: Müftüoğlu S, Kaymaz F, Atilla P. Ankara: Palme Yayıncılık, 2009: 278-82.
Junqueria LC, Carneiro J. Temel Histoloji. In: Aytekin Y, Solakoğlu S. ed.8.baskı. İstanbul: Barış Kitapevi 1998: 280-7.
Ross, MH, Pawlina W. Histology A Text and Atlas With correlated cell and molecular biology (5 ed). Baltimore: Lip- pincott Williams & Wilkins, 2006: 522-34.
Abraham L, Kierszenbaum A. Histoloji ve hücre biyolojisi, patolojiye giriş. In: Demir R. Ankara: Palme Yayıncılık, 2006:
Charlotte L. Ownby Veterinary Histology VMED 7123 Fall Semester 2004:4-5
Carslon BM. Human embryology and developmental and developmental biology, fourth edition, Mosby Elsevier, Phi- ladelphia: 2009: 362-64.
Ergun GA, Miskovitz MD. Aging and the esophagus: Com- mon pathologic conditions and their effect upon swallowing in the geriatric population. Dysphagia 1992; 7: 58-63.
Gregersen H, Lu X, Zhao J. Physiological growth is associated with osephageal morphometric and biomechanical changes in rats. Neurogastroenterol Motil 2004; 16: 403-12.
Hollander D, Tarnawski A, Stachura J, Gergely H. Morpholo- gic changes in gastricrmicosa of aging rats. Dig Dis Sci 1989; 34: 1692-700.
Menard D, Arsenault P. Maturation of human fetal esophagus maintained in organ culture. Anat Rec 1987; 217: 384-54.
Sorkun HC, Özadamar S. A Study on the Prenatal and postna- tal development of rat esophagus. T Klin J Med Sci 2002; 22.
Cerimele D, Celleno, Serri F. Physiological changes in aging skin. Br J Dermatol 1990;13-20.
Allı N. Deri yaşlanmasında hücresel ve moleküler mekanizmalar. T Kin J Kozmetoloji 1998; 1296-99.
Tuncer I, Tosun M, Kalkan S, et al. Histomorphologic Evalua- tion of the development of the esophagus Between 17 and 32 Weeks old human fetusus. Erciyes Medical Journal 2005; 27: 152-7.
de Souza RR, Moratelli HB, Borges N, Liberti EA. Age- induced nerve cell loss in the myenteric plexus of the small in- testine in man. Gerontology 1993; 39: 183-8.
Eckardt VF, LeCompte PM, Volker F, Eckard T, Philip M. LeCompte Esophageal ganglia and smooth muscle in the elderly. Am J Dig Dis 1978; 23: 443-8.
Ekinci N. Deney Hayvanları Anatomisi. 22-30 Ocak 2010 Deney Hayvanları Kullanım Sertifikası Eğitim Kursu Sunumu. Malatya: 2010.
De Lemos C. The ultrastructure of endocrine cells in the corpus of the stomach of human fetuses. J Anat 1997; 148: 359-84.
Helander H. Ultrastructure and function of gastric parietal calls in the rat during development. Gastroenterology 1969; 56: 35-52.
Penttıla A. The fine structure and dihydroxyphenylalanine uptake of the developing parietal cells of the rat Stomach. Z Anat. Entwickl 1970; 132: 34-49.
Kammaraad A. The development of the gastrointestinal tract of the rat. Histogenesis of the epithelium of the stomach, small intestine and pancreas. J Morphol 1942; 70: 323.
Goldstein I, Reece EA, Yarkoni S, Wan M, Gren JL, Hobbins JC. Growth of the fetal stomach in normal pregnancies. Obstet Gynecol 1987; 70: 641-44.