Doksorubisin Kardiyotoksisitesinde Timokinonun Kan Parametreleri Üzerine Etkileri

Bu çalışma Doksorubisinin (DOX) neden olduğu kardiyotoksisitede meydana gelen biyokimyasal parametre değişiklikleri üzerine timokinonun etkilerini araştırmak amacıyla yapılmıştır. Çalışmamızda 18 adet Wistar albino erkek sıçan kullanıldı. Sıçanlar kontrol, doksorubisin ve doksorubisin+timokinon (DOX+TQ) grubu olacak şekilde üç gruba ayrıldı. Kontrol grubu deneyin 5. gününde kuyruk veninden tek seferlik serum fizyolojik; DOX grubu deneyin 5. gününde kuyruk veninden tek doz 45 mg/kg DOX; DOX+TQ grubu yedi gün boyunca gavajla 50 mg/kg timokinon ve deneyin 5. gününde kuyruk veninden tek doz 45 mg/kg DOX aldı. Sıçanlar deneyin son gününde ketamin-ksilazin anestezisi altında sakrifiye edilerek total kan örnekleri alındı ve biyokimyasal parametreler değerlendirildi. Biyokimyasal verilere göre, kontrol grubuna kıyasla DOX grubunda kreatin kinaz miyokardiyal bant (CK-MB), interlökin- 6 (IL-6), interlökin-18 (IL-18), total oksidan seviye (TOS) ve malondialdehit (MDA) değerleri artarken total antioksidan seviye (TAS) değeri düşmüştür. DOX grubuna göre, DOX+TQ grubunda CK-MB, IL-6, IL-18, TOS ve MDA değeri düşerken, TAS değeri artmıştır. Çalışma sonuçlarımız, doksorubisinin kan parametrelerinde neden olduğu bozulmanın timokinon tarafından düzeltilebileceğini göstermektedir.

Anahtar Kelimeler:

Doksorubisin, Timokinon, Kardiyotoksisite

Effects of Thymoquinone on Blood Parameters in Doxorubicin Cardiotoxicity

This study was conducted to investigate the effects of thymoquinone on biochemical parameter changes in cardiotoxicity caused by doxorubicin (DOX). 18 Wistar albino male rats were used in our study. The rats were divided into three groups as control, doxorubicin and doxorubicin + thymoquinone (DOX + TQ) group. The control group received one-time saline from the tail vein on the 5th day of the experiment. On the 5th day of the experiment, the DOX group received a single dose of 45 mg / kg DOX from the tail vein. The DOX + TQ group received 50 mg / kg thymoquinone by gavage for seven days and a single dose of 45 mg / kg DOX from the tail vein on the 5th day of the experiment. Rats were sacrificed under ketamine-xylazine anesthesia and total blood samples were taken and biochemical parameters were evaluated on the last day of the experiment. According to the biochemical data, in DOX group in while the creatine kinase myocardial band (CK-MB), interleukin-6 (IL-6), interleukin-18 (IL-18), Total Oxidant Status (TOS) and malondialdehyde (MDA) levels increased, the Total Antioxidant Status (TAS) decreased compared to the control group. Compared to the DOX group, while the value of CK-MB, IL-6, IL-18, TOS and MDA decreased, TAS increased in the DOX + TQ group. Results of our study shows that the deteriorative effects of doxorubicin on blood parameters can be corrected by thymoquinone.

Keywords:

Doxorubicin, Thymoquinone, Cardiotoxicity,

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Experimental and Applied Medical Science-Cover

ISSN: 2757-847X
Başlangıç: 2020
Yayıncı: Gaziantep İslam Bilim ve Teknoloji Üniversitesi

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