D. Fulya KIZILGEDİK, Armagan CANER, Çağlar YILDIZ, Buğra OKŞAŞOĞLU, Sema MISIR, İlhan YAYLIM, Semra DEMOKAN, Ceylan HEPOKUR

Investigation of Galectin-3 Levels of Endometriosis Patients According to Stages

Objective: Endometriosis is a gynecological disease associated with chronic pelvic inflammation, pain, and infertility. Galectin-3 (Gal-3) is a protein that can bind to β-galactosides, which plays an important role in different biological processes according to the stages of the disease in patients with endometriosis. This study aimed to elucidate the importance of Gal-3 in endometriosis, to reveal its potential power as a non-invasive diagnostic biomarker in disease etiopathogenesis. Materials and Methods: The serum concentration of Gal-3 and cancer antigen 125 (CA-125) from whole blood were measured using enzyme-linked immuno sorbent assay (ELISA) and an auto-analyzer, respectively. Gal-3 expression was determined by quantitative real-time polymerase chain reaction (qRT-PCR) from peripheral blood. Results: We found significant differences for Gal-3 expression levels between the endometriosis and control groups (p <0.05). Gal-3 levels in the serum of women with endometriosis are also remarkably increased compared with the control group. Conclusion: Galectin-3 can play critical functions in the development and progression of endometriosis, so, further studies are needed in this area

Keywords:

Galectin-3, endometriosis, biomarkers, qRT-PCR,

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1. Bulun SE. Mechanisms of disease endometriosis. N Engl J Med 2009; 360: 268-79. [CrossRef] google scholar
2. Pabalan N, Jarjanazi H, Christofolini DM, Barbosa CP, Bianco B. Association of the intercellular adhesion molecule-1 (ICAM-1) gene polymorphisms with endometriosis: A systematic review and metaanalysis. Arch Gynecol Obstet 2015; 292: 843-51. [CrossRef] google scholar
3. Meggyes M, Szereday L, Bohonyi N, Koppan M, Szegedi S, Marics-Kutas A et al. Different expression pattern of TIM-3 and Galectin-9 molecules by peripheral and peritoneal lymphocytes in women with and without endometriosis. Int J Mol Sci 2020; 21(7): 2343. [CrossRef] google scholar
4. Aghajanpour L, Mashayekhi F, Rajaei F. Intercellular adhesion molecule-1 (ICAM-1) gene polymorphism and endometriosis in northern Iran. Arch Gynecol Obstet 2011; 283:1035-9. [CrossRef] google scholar
5. Caserta D, Di Benedetto L, Bordi G, D’Ambrosio A, Moscarini M. Levels of Galectin-3 and stimulation expressed gene 2 in the peritoneal fluid of women with endometriosis: A pilot study. Gynecol Endocrinol 2014; 30(12): 877-80. [CrossRef] google scholar
6. Sharpe Timms KL. Endometrial anomalies in women with endometriosis. Ann NY Acad Sci 2001; 943: 131-47. [CrossRef] google scholar
7. Bastón JI, Barañao RI, Ricci AG, Bilotas MA, Olivares CN, Singla JJ. Targeting galectin-1-induced angiogenesis mitigates the severity of endometriosis. J Pathol 2014; 234(3): 329-37. [CrossRef] google scholar
8. Crosignani P, Olive D, Bergqvist A, Luciano A. Advances in the management of endometriosis: An update for clinicians. Hum Reprod Update 2006; 12: 179-89. [CrossRef] google scholar
9. Heidemann LN, Hartwell D, Heidemann CH, Jochumsen KM. The relation between endometriosis and ovarian cancer - a review. Acta Obstet Gynecol Scand 2014; 93(1): 20. [CrossRef] google scholar
10. Cho S, Mutlu L, Grechukhina O, Taylor HS. Circulating microRNAs as potential biomarkers for endometriosis. Fertil Steril 2015; 103(5): 1252-60. [CrossRef] google scholar
11. Van den Brûle FA, Fernandez PL, Buicu C, Liu FT, Jackers P, Lambotte R, et al. Differential expression of galectin-1 and galectin-3 during first trimester human embryogenesis. Dev Dyn 1997; 209: 399405. [CrossRef] google scholar
12. Fowlis D, Colnot C, Ripoche MA, Poirier F. Galectin-3 is expressed in the notochord, developing bones, and skin of the post implantation mouse embryo. Dev Dyn 1995; 203(2): 241-51. [CrossRef] google scholar
13. Kyama CM, Debrock S, Mwenda JM, D’Hooghe TM. Potential involvement of the immune system in the development of endometriosis. Reprod Biol Endocrinol 2003; 1: 123. [CrossRef] google scholar
14. Brubel R, Bokor A, Pohl A, Schilli GK, Szereday L, Bacher-Szamuel R et al. Serum galectin-9 as a noninvasive biomarker for the detection of endometriosis and pelvic pain or infertility-related gynecologic disorders. Fertil Steril 2017; 108(6): 1016-25. [CrossRef] google scholar
15. F O Dorien, Flores Idhaliz, Waelkens E, D’Hooghe T. Noninvasive diagnosis of endometriosis: Review of current peripheral blood and endometrial biomarkers. Best Pract Res Clin Obstet Gynaecol 2018; 50: 72-83. [CrossRef] google scholar
16. Fassbender A, Burney RO, O DF, D’ Hooghe T, Giudice L. Update on biomarkers for the detection of endometriosis. BioMed Res Int 2015; 2015: 130854. [CrossRef] google scholar
17. May KE, Conduit-Hulbert SA, Villar J, Kirtley S, Kennedy SH, Becker CM. Peripheral biomarkers of endometriosis: A systematic review. Hum Reprod Update 2010; 16: 651-74. [CrossRef] google scholar
18. Noël JC, Chapron C, Borghese B, Fayt I, Anaf V. Galectin-3 is overexpressed in various forms of endometriosis. Appl. Immunohistochem Mol Morphol. 2011; 19: 253-7. [CrossRef] google scholar
19. Vergetaki A, Jeschke U, Vrekoussis T, Taliouri E, Sabatini L, Papakonstanti EA, et al. Galectin-1 overexpression in endometriosis and its regulation by neuropeptides (CRH, UCN) indicating its important role in reproduction and inflammation. PLoS One 2014; 9: e114229. [CrossRef] google scholar
20. Brinchmann MF, Patel DM, Iversen MH. The role of Galectins as modulators of metabolism and inflammation. Mediat Inflamm 2018: 9186940. [CrossRef] google scholar
21. Mattos RM, Machado DE, Perini JA, Alessandra-Perini J, Costa Nathália de OM, Oliveira Wiecikowsk AFR, et al. Galectin-3 plays an important role in endometriosis development and is a target to endometriosis treatment. Mol Cell Endocrinol 2019; 486: 1-10. [CrossRef] google scholar
22. Dong R, Zhang M, Hu Q, Zheng S, Soh A, Zheng Y, Yuan H. Galectin-3 as a novel biomarker for disease diagnosis and a target for therapy (Review). Int J Mol Med 2018; 41(2): 599-614. [CrossRef] google scholar
23. Hisrich BV, Young RB, Sansone AM, Bowens Z, Green LJ, Lessey Bruce A, et al. Role of human galectins in inflammation and cancers associated with endometriosis. Biomolecules 2020; 10(2): 230. [CrossRef] google scholar
24. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta C(T)) method. Methods 2001; 25(4): 402-8. [CrossRef] google scholar
25. Rekker K, Saare M, Roost AM, Kaart T, Söritsa De, Karro H, et al. Circulating miR-200-family micro-RNAs have altered plasma levels in patients with endometriosis and vary with blood collection time. Fertil Steril 2015; 104(4): 938-46. [CrossRef] google scholar
26. Hsu AL, Khachikyan I, Stratton P. Invasive and non-invasive methods for the diagnosis of endometriosis. Clin Obstet Gynecol 2010; 53(2): 413-9. [CrossRef] google scholar
27. Meeusen JW, Johnson JN, Gray A, Wendt P, Jefferies JL, Jaffe AS, et al. Soluble ST2 and galectin-3 soluble ST2 and galectin-3 in pediatric patients without heart failure. Clin Biochem 2015; 48: 1337-40. [CrossRef] google scholar
28. Schindler EI, Szymanski JJ, Hock KG, Geltman EM, Scott MG: Short-and long-term biologic variability of galectin-3 and other cardiac biomarkers in patients with stable heart failure and healthy adults. Clin Chem 2016; 62: 360-6. [CrossRef] google scholar
29. Johannes L, Jacob R Leer H. Galectins at a glance. J Cell Sci 2018; 131. [CrossRef] google scholar
30. Von Wolff M, Wang X, Gabius HJ, Strowitzki T. Galectin fingerprinting in human endometrium and decidua during the menstrual cycle and in early gestation. Mol Hum Reprod 2005; 11: 189-94. [CrossRef] google scholar
31. Yang H, Yin J, Ficarrotta K, Hsu SH, Zhang W, Cheng C. Aberrant expression and hormonal regulation of Galectin-3 in endometriosis women with infertility. J Endocrinol Invest 2016; 39(7): 785-91. [CrossRef] google scholar
32. De Mattos RM, Pereira PR, de Oliveira Barros EG, da Silva JH, Palmero CY, da Costa NM, et al. Aberrant levels of Wnt/ß-catenin pathway components in a rat model of endometriosis. Histol Histopathol 2016; 31: 933-42. google scholar