Fatma KALAYCI YÜKSEK, Defne GÜMÜŞ, Gül İpek GÜNDOĞAN, Aysun UYANIK ÖCAL, Nur ELAGÜL, Mine ANĞ KÜÇÜKER

8620

Cross-interactions between Norepinephrine, Methicillin-Resistant Staphylococcus aureus and Human Osteoblast Cells in Culture Conditions

Objective: The role of norepinephrine (NE) on growth, adhesion and invasion of methicillin-resistant Staphylococcus aureus (MRSA) ATTC 43300 was examined in human osteoblast (HOB) cells. The effects of NE and/or MRSA on the viability and cell death pathways of HOB cells were also investigated. Furthermore, the alterations of bacterial response to oxidative stress (H2O2) were analyzed in the presence/absence of NE. Materials and Methods: Bacterial growth was detected spectrophotometrically. The colony counting method was examined for adhesion-invasion. The alteration of HOB cell viability was determined by methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay. The death pathways of HOB cells were examined microscopically using acridine orange-ethidium bromide dual staining and dichlorofluorescein-diacetate (DCF-DA) dye. The bacterial response to H2O2 was investigated by agar dilution.Results: The growth of bacterium was not affected in the presence of NE. Bacterial adhesion was decreased by NE (p<0.0001) while high-level NE induced invasion (p=0.013). HOB cell viability was reduced by MRSA and/or NE (p<0.001). MRSA and co-existence of MRSA and NE caused necrosis more than apoptosis in HOB cells (p<0.05). NE did not alter the bacterial response to oxidative stress. Conclusion: Norepinephrine has different effects on the biological properties of both MRSA and HOB cells.
Keywords:

Norepinephrine MRSA, growth, adhesion-invasion, oxidative stress response, HOB cell viability, apoptosis-necrosis,

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Experimed-Cover
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 2011
  • Yayıncı: İstanbul Üniversitesi
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