Sacide PEHLİVAN, Ali AYDENİZ, Tuğçe SEVER, Özlem ALTINDAĞ, Sibel OĞUZKAN-NALCI, Türkan HARUNLAR

No association between myeloperoxidase gene G-463A polymorphism and rheumatoid arthritis

Miyeloperoksidaz (MPO) ve genetik polimorfizmleri Romatoid Artrit (RA) gibi reaktif oksijen türlerinin aşırı arttığı çeşitli hastalıkların patogenezinde rol oynamaktadır. Bu çalışmada MPO G463A polimorfizminin RA ile ilişkisinin olup olmadığı araştırıldı. Çalışma kapsamında 75 hasta ve 90 sağlıklı kontrol birey analiz edildi. Genotiplendirme polimeraz zincir reaksiyonu ve restriksiyon enzimi uzunluk polimorfizmi (PCR-RFLP) yöntemi ile yapıldı. Bu tek nükleotid polimorfizmi (SNP) ve RA arasındaki ilişki ki-kare testi ve de-finetti programları kullanılarak analiz edildi. Kontrol grubu ve RA hasta grubu arasında genotip dağılımı ve allel sıklıkları bakımından istatistiksel olarak anlamlı bir fark yoktu. Bunun yanı sıra gruplarda "Hardy-Weinberg Dengesi"nden sapmanın olmadığı saptandı (p>0.05). MPO geni G-463A polimorfizmi ile RA arasındaki ilişkinin ilk defa analiz edildiği bu çalışma sonucunda Türk RA hastalarında bu ilişkinin olmadığı sonucuna varıldı. Bir sonraki aşamada MPO genindeki diğer polimorfizmlerin çalışılması planlanmaktadır.

Anahtar Kelimeler:

Peroksidaz, Polimorfizm, genetik, Artrit, romatoid, Polimorfizm, kesim parçacığı uzunluk, Polimeraz zincir reaksiyonu

Miyeloperoksidaz geni G-463A polimorfizm ile romatoid artrit arasında bir ilişki yoktur

Myeloperoxidase (MPO) has been involved in the pathogenesis of several diseases such as rheumatoid arthritis (RA) through excessive production of reactive oxygen species (ROS) as well as through its genetic polymorphism. We examined whether G-463A polymorphism of Myeloperoxidase (MPO) gene was associated with RA. Exactly, 75 patients'with RA and 90 healthy control subjects were included in this study. The genotyping was determined by polymerase chain reaction-restriction fragment length polymorphism method. The association between these single nucleotide polymorphism (SNP) and RA was analyzed using chi-square test and de-Finetti program. Genotype distributions and allele frequency of RA patients were not significantly different from healthy controls. In addition, it was also determined that there.was no deviation from Hardy-Weinberg Equilibrium in any groups (p>0.05). Whether there was an association between MPO gene G-463A gene polymorphism and RA was investigated for the first time in this study in literature and it was demonstrated that it did not exist in the Turkish- RA patients. It'was planned to investigate the other polymorphisms of MPO gene in the future.

Keywords:

Peroxidase, Polymorphism, Genetic, Arthritis, Rheumatoid, Polymorphism, Restriction Fragment Length, Polymerase Chain Reaction,

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