Afaf ELSAİD, Mohamed HELALY, El-sayed HATATA, Osama FOUDA, Ahmad SETTİN

TNF-α-308 and INF-γ+874 Gene Polymorphisms in Relation to Susceptibility and Severity of Type 2 Diabetes Mellitus among Egyptian patients

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TNF-α-308 and INF-γ+874 Gene Polymorphisms in Relation to Susceptibility and Severity of Type 2 Diabetes Mellitus among Egyptian patients

To explore the association of TNF-α-308 and IFN- γ +874 genetic polymorphisms with type 2 diabetes mellitus (DM) in Egyptian cases. Participants included 207 cases with type 2 DM, 93 males and 114 females. Their mean age was 57 years. They were compared to a control group of 212 healthy unrelated subjects from the same locality. DNA was amplified using PCR with sequence specific primers for detection of polymorphism related to TNF-α-308 (G/A) and IFN-γ+874 (A/T). The combined homozygote pattern of TNF-α-308 (AA) and IFN-γ+874 (A/A) was significantly higher in diabetic cases versus controls (p=0.023) that was manifest (although non-significant) among complicated, uncontrolled cases and cases with marked insulin resistance. Polymorphism related to TNF-α-308 AA and IFN-γ+874 AA may be considered as a genetic biomarker for type 2 DM in Egyptian subjects with potential impact on family counseling and management.

Keywords:

TNF-α-308, IFN- γ +874, type 2 DM, gene polymorphism Egypt Mısırlı,

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