Gülcan KURTİPEK SAYLAM, Recep KESLİ, Caner KUTLUGÜN

Investigation of the role of inluenza viruses A-B, parainfluenza viruses 1-3, respiratory syncytial virus and adenovirus in the etiology of pityriasis rosea by DNA-hybridization method

Amaç: Pitriazis rozea (PR) sebebi bilinmeyen ve enfeksiyon ile ilişkili olduğu en fazla düşünülen akut inflamatuar bir cilt hastalığıdır. Bu çalışmada, PR’li hastalarla influenza virus A-B, parainfluenza virus 1-3, respiratuar sinsitiyal virus ve adenovirusün etyolojideki rolünün araştırılması amaçlandı. Metod: Influenza virus A-B, parainfluenza virus 1-3, respiratuar sinsitiyal virus ve adenoviruslerin PR etiyolojisi ile ilişkisinin var olup olmadığı konusunu aydınlatmak üzere her biri otuz adet olmak üzere, PR tanısı konmuş hastalardan ve kontrol grubundan elde edilen nazofaringeal sürüntü örneklerinde bu viruslara ait RNA ve DNA materyalinin varlığı DNA-hibridizasyon yöntemi ile araştırıldı. Bulgular: Hastaların ve kontrol grubunun hiçbirinde influenza virus A-B, parainfluenza virus 1-3, respiratuar sinsitiyal virusa ait RNA ve adenovirusa ait DNA pozitifliği belirlenmedi. Sonuç: Sonuç olarak hastalığın etiyolojisinin viral olup olmadığı hala bilinmemektedir,bu nedenle hastalığın etyolojisinin aydınlatılması için daha ileri çalışmaların yapılması gereklidir.

İnluenza viruses A-B, parainfluenza viruses 1-3, respiratory syncytial virus ve adenovirus'ün pityriasis rosea etyolojisinde DNA-hybridizasyon methodu ile rollerinin araştırılması

Aim: Pityriasis Rosea (PR) is an acute inflamatory skin disease with unknown etiology considered to be the most associated with infections. In this study, it was aimed to investigate the role of influenza viruses A-B, parainfluenza viruses 1-3, respiratory syncytial virus (RSV) and adenovirus in the etiology of PR patients. Method: Thirty out-patients, applying to the dermatology department of Konya Education and Research Hospital, who were diagnosed with PR, and healty PR negative control group at the same number, age and sex with PR patients were included into the study. In order to enlighten whether an association of PR with the influenza viruses A-B, parainfluenza viruses 1-3, RSV and adenovirus viruses existed or not. RNA materials from influenza viruses A-B, parainfluenza viruses 1-3 and RSV; DNA material from adenovirus were investigated by using DNA reverse-hybridization method in the nasofarengeal samples obtained from patients with PR and control groups included to the study. Result: No RNA positivity was determined, related to influenza viruses A-B, parainfluenza viruses 1-3, RSV and DNA to adenovirus in any of the patients and control groups. Conclusion: As a consequence, viral etiology of the PR disease still remains unknown, whether to be viral or not, thus further studies are needed to enlighten the unknown etiology of the disease.

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