A. KİMİRAN ERDEM, E. Ö. ARSLAN AYDOĞDU, G. CEVAHİR ÖZ, Ç. EROL, M. YAZGAN

Farklı Amsonia orientalis Decne (Apocynaceae) ekstraktlarının antibakteriyel ve hemolitik aktiviteleri

Çalışmamızda Amsonia orientalis Decne. (Mavi Yıldız)’ nin ham ekstraktlarının (gövde ve yaprağın kloroform ve metanol; yaprağın hekzan, aseton, deiyonize su ve etanol; gövde dokusunun deiyonize su ekstraktları) 17 farklı Gram-negatif ve Gram-pozitif bakteriye karşı antibakteriyel aktiviteleri incelenmiştir. Ekstraktların antibakteriyel ve hemolitik etkileri sırasıyla standart antibiyotik diskler ve insan eritrositleri kullanılarak değerlendirilmiştir. Yaprakların hekzan ekstraktı ile yaprak+gövde dokusunun kloroform ekstraktı dışındaki tüm ekstraktlarda antibakteriyel aktivite saptanmıştır. Pseudomonas aeruginosa ATCC 9327, Staphylococcus aureus ATCC 6538, Enterococcus faecalis ATCC 10541 ve Proteus mirabilis’ in denenen ekstraktlara karşı üreme inhibisyon zonu oluşturmadığı; Bacillus subtilis ATCC 6633, Legionella pneumophila ATCC 33152 ve L. pneumophila SG 2-14’ ün ise inhibisyon zonu oluşturduğu saptanmıştır. Yaprağın etanol (≥5 mg/ml), aseton (≥10 mg/ml) ve deiyonize su (≥20 mg/ml) ekstraktları ile yaprak+gövde dokusunun metanol (≥10 mg/ml) ekstraktı hemolitik aktivite gösterirken, yaprak+gövde dokusunun kloroform, yaprağın hekzan ve gövde dokusunun deiyonize su ekstraktlarının hemolitik aktivite göstermediği saptanmıştır.

Anahtar Kelimeler:

Amsonia orientalis, Antibakteriyel aktivite, Hemolitik aktivite, Bitki ekstraktı

Antibacterial and hemolytic activities of different extracts of Amsonia orientalis Decne (Apocynaceae)

In the current study, the antibacterial activities of the crude extracts (leaf and stem chloroform and methanol; leaf hexane, acetone, deionized water and ethanol; stem deionized water) of Amsonia orientalis Decne. (Blue Star) were investigated against 17 different strains of Gram-negative and Gram-positive bacteria. To evaluate the antibacterial and hemolitic activities of extracts standard antibiotic discs and human erythrocytes were used, respectively. The antibacterial activity was determed in the all extracts, except the hexane extracts of the leaves. It was observed that Bacillus subtilis ATCC 6633, Legionella pneumophila ATCC 33152 and L. pneumophila serogroup (SG) 2-14 are the most susceptible bacteria to the different extracts, while Pseudomonas aeruginosa ATCC 9327, Staphylococcus aureus ATCC 6538, Enterococcus faecalis ATCC 10541 and Proteus mirabilis are resistant bacteria. It was determined that chloroform (leaf and stem), hexane (leaf) and deionized water (stem) extracts did not show hemolytic activity, while ethanol (leaf) (â‰¥5 mg/ml), methanol (stem and leaf) (â‰¥10 mg/ml), acetone (leaf) (â‰¥10 mg/ml) and deionized water (leaf) (â‰¥20 mg/ml) extracts showed hemolytic activity.Key words: Amsonia orientalis, Antibacterial activity, Hemolytic activity, Plant extract.*Corresponding Author: Elif Özlem ARSLAN AYDOĞDU (e-mail: eoarslan@istanbul.edu.tr)(Received: 01.03.2012 Accepted: 21.09.2012)

Keywords:

Amsonia orientalis, Antibacterial activity, Hemolytic activity, Plant extract.,

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Akyalçın H., Özen F. and Dülger B. (2006) Anatomy, Morphology, Palynology and Antimicrobial Activity of Amsonia orientalis Decne. (Apocynaceae) Growing in Turkey. International Journal of Botany, 2: 93-99.
Arslan-Aydoğdu E.Ö. and Çotuk A. (2008) Antibacterial and Hemolytic Activity of the Coelomic Fluid of Dendrobaena veneta (Oligochaeta, Lumbricidae) Living in Different Localities, IUFS Journal of Biology, 67: 23-32. Barry A.L. and Thornsberry C. (1981) Susceptibility testing: in manual of clinical microbiology, pp. 561-574 in E.H. Lennette, A. Balows, W.J. Hausler, J.P. Truant (Eds): American Society for Microbiology. Washington D.C.
Clauder O., Kocsis A., Bojtheho K., Mathe I. and Tamas J. (1973) Alkaloids of Amsonia angustifolia. Acta Chimica Hungarica, 75: 423-4
Clinical and Laboratory Standards Institute, Performance Standars for Antimicrobial Susceptibility Testing, http://www.clsi.org/ (December, 2012).
Dabine-Lengyel E., Turiak G.Y., NyaradinéSzabady J., Zambo I., Tétényi P. and Hermecz I. (1986) Determination of Secologanin Content from Shoots of Rhazya orientalis (Decne) A. DC. Using the Hplc Method. Herba Hungarica, Tom, 25: 141-150.
Davis P.H. (1978) Flora of Turkey and East Aegean Islands. University Press of Edinburg. Edinburg. Dedicoat M. and Venkatesan P. (1999) The treatment of Legionnaires’ disease. Journal of Antimicrobial Chemotherapy, 43:747-752.
Dushenkov V. and Raskin I. (2008) New Strategy for the Searchof Natural Biologically active Substances. The Russian Journal of Plant Physiology, 55: 564-567.
Evans D.A., Smith G.F. and Stapleford G.N. (1968) Rhazya alkaloids: the secamines, a new group of indole alkaloids. Chemical Communications, 15: 859-861.
Iqbal S., Bhanger M.I., Akhtar M., Anwar F., Ahmed K.R. and Anwer T. (2008) Antioxidant properties of methanolic extracts from leaves of Rhazya stricta. Journal of Medicinal Food, 9: 270-275.
Itoh A., Kumashiro T., Tanahashi T., Nagakura N. and Nishi T. (2002) Flavonoid Glycosides from Rhazya orientalis. Journal of Natural Products, 65: 352-357.
Kimiran-Erdem A., Sanli-Yürüdü N.Ö. and Cotuk A. (2007) Efficacy of a quaternary ammonium compound against planktonic and sessile populations of different Legionella pneumophila strains. Annual Microbiology, 57: 121-126.
Kohlerova P., Beschin A., Silerova M., Baetselier P.D. and Bilej M. (2004) Effect of experimental microbial challenge on the expression of defense molecules in Eisenia foetida earthworm. Developmental and Comparative Immunology, 28: 701-711.
Konar V., Yılmaz Ö., Öztürk A.I., Kırbağ S. and Arslan M. (2000) Antimicrobial and biological effects of bomphos and phombhos on bacterial and yeast cells. Bioorganic Chemistry, 28: 2142
Ozaki Y. (1989) Pharmacological studies of indole alkaloids obtained from domestic plants, Uncaria rhynchophylla Miq. and Amsonia elliptica Roem. et Schult. Nippon Yakurigaku Zasshi, 94: 17–26.
Ozaki Y. (1990) Vasodilative effects of indole alkaloids obtained from domestic plants, Uncaria rhynchophylla Miq. and Amsonia elliptica Roem. et Schult. Nippon Yakurigaku Zasshi, 95: 47-54.
Ozaki Y. (1994) Pharmacological Studies of Indole Alkaloids Obtained from Domestic Plants, Uncaria rynchophylla Miq. and Amsonia elliptica Roem et Schult. Nippon Yakurigaku Zasshi, 1: 17–26.
Özen F. (2006) Türkiye’de Tükenme Tehlikesinde Olan Bir Türün Otekolojisi: Amsonia orientalis Decne. (Apocynaceae). Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 8: 4-9.
Öztürk A.I., Yılmaz Ö., Kırbağ S. and Arslan M. (2000) Antimicrobial and biological effects of ipemphos and amphos on bacterial and yeast strains. Cell Biochemistry and Function, 18: 117-1
Tanira M.O.M., Ali B.H., Bashir A.K. and Chandranath I. (1996) Some pharmacologic and toxicologic studies on Rhazya stricta (Decne) in rats, mice and rabbits. General Pharmacology, 27: 1261-1267.
Tutin T.G., Heywood V.H., Burges N.A., Moore D.M., Valentine D.H., Walters S.M. and Webb D.A. (1968) Flora Europaea. Cambridge University Press. Cambridge.
Zongo C., Akomo E.F.O., Savadogo A., Obame L. C., Koudou J. and Traore A.S. (2009) In vitro antibacterial properties of total alkaloids extract from Mitragyna Inermis (Wild.) O. Kuntze, a west African traditional medical plant. Asian Journal of Plant Sciences,8 (2): 172-177.
Zsadon B., Décsei L., Otta K., Szilasi M. and Kaposi P. (1974) Isolation of aspidosperma alkaloids and rutin from Amsonia tabernaemontana Walt. Acta Pharmaceutica Hungarica, 1: 74-797.
Zsadon B., Tamas J., Szilasi M. and Kaposi P. (1973) Indole and Indole Derivatives Part 13 Levo Vincadine and Racemic Vincadine from the Leaves of Amsonia-Tabernaemontana. Acta Chimica Academiae Scientiarum Hungaricae, 78: 207-16.

ISSN: 2602-2575
Yayın Aralığı: Yılda 2 Sayı
Başlangıç: 1940
Yayıncı: İstanbul Üniversitesi Yayınevi

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