Mine yüzey pürüzlülüğü üzerine yapay gastrik sıvının etkisinin in vitro olarak incelenmesi

Bu in vitro çalışmanın amacı; yapay gastrik sıvı içerisinde 10 dakika ve 24 saat bekletildikten sonra mine yüzey pürüzlülüğünde meydana gelen değişikliklerin incelenmesidir. Çalışmada 12 adet sürmemiş, insan 3. molar dişi kullanıldı. Dişler, çekim sonrası üzerindeki doku artıkları ve debrislerden arıtıldı, distile suda yıkandı ve otopolimerizan akrilik rezine gömüldü. Örnekler; bukko-lingual yönde ortadan ikiye bölündü Microcut, Metkon, Turkey , bir yarısı kontrol grubu diğer yarısı da deney grubu olarak ayrıldı. Dişlerin bukkal yüzeyleri, düzgün mine yüzeyleri elde etmek için su altında 600–1200 grenli silikon karbid kağıtlar kullanılarak düzeltildi. Örneklerin başlangıç yüzey pürüzlülük değerleri ölçüldü Perthometer M2, Germany . Daha sonra deney grubundaki örnekler yapay gastrik sıvıda pH 1,14 , kontrol grubundaki örnekler ise distile suda 10 dakika ve 24 saat bekletildi ve her bir bekletme süresini takiben yüzey pürüzlülük değerleri ölçüldü. Paired t testi ile yapılan istatistiksel inceleme sonucuna göre; kontrol ve deney gruplarının yüzey pürüzlülükleri arasındaki fark anlamlı bulunmuştur. Pürüzlülük değerlerinin zamana bağlı değişimini incelemek amacıyla yapılan paired t testi analizi sonucunda deney grubunda 0, 10. dakika ve 24. saat ölçümleri arasındaki fark anlamlı bulunurken; kontrol grubunda herhangi bir fark gözlenmemiştir. Dişlerin gastrik sıvıyla kısa ve uzun süreli olarak temas etmesi sonucunda mine yüzey pürüzlülüğünde önemli bir artış gözlenmektedir

Anahtar Kelimeler:

Gastrik sıvı, yüzey pürüzlülüğü, gastroözofageal reflü, erozyon

In vitro Investigation of Simulated Gastric Juice Effects on Surface Roughness of Enamel

The objective of this in vitro study was to measure changes in surface roughness of enamel after immersion in simulated gastric juice. Twelve unerupted and freshly extracted human third molars were used. Teeth were scraped of any remaining debris, washed in distilled water and embeded into acrylic resin. The specimens were divided bucco-lingually into two pieces, one half served as a control, and the other half as experimental. Buccal side of the tooth specimens was assinged as enamel and ground wet to achive a flat enamel surface using 600, 1200 grid siliconecarbid paper. Baseline surface profiles of the enamel specimens were recorded. Then the experimental specimens were stored in simulated gastric juice and the control specimens were stored in distilled water for 10 min and 24 h. the surface roughness was measured after each exposure. According to paired-t-test, the differences between surface roughness of experimental group and control group were statistically significant. Paired-t-test used for analysing the change of roughness measures against time showed that significant differences occured in values of experimental group at baseline and at 10 min and at 24 h. there was no significant difference for control group. During short and long period of contact with gastric juice significantly reduced roughness of enamel.

Keywords:

Gastric juice, surface roughness, gastroeosophageal reflux, erosion,

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1. Lazarchik DA, Filler SJ. Effects of gastroesophageal reflux on the oral cavity. Am J Med. 1997; 103 : 107-13.
2. Pindgorg JJ. Chemical and physical injuries. In: Pindgorg JJ. Pathology of the dental hard tissues. Phil: WB Saunders, 1970; p. 312-325.
3. Hemingway CA, Parker DM, Addy M, Barbour ME. Erosion of enamel by non-carbonated soft drinks with and without toothbrushing abrasion. Br Dent J 2006; 201: 447-50.
4. Scheutzel P. Etiology of dental erosionintrinsic factors. Eur J Oral Sci 1996; 104: 178-90.
5. Colin-Jones DG. Gastroesophageal reflux disease. Prescr J 1996; 36: 66-72.
6. Dabsban A, Patel H, Delaney J, Wverth A, Thomas R, Tolia V. Gastroesophageal reflux disease and dental erosion in children. J Pediatr 2002; 23: 474-8.
7. Lazarchik DA, Filler SJ. Dental erosion: predominant oral lesion in gastroeosophageal reflux disease. Am J Gastroenterol 2000; 95: 33-8.
8. Jarvinen V, Meurman JH, Hyvarinen H, Rytömaa I, Murtomaa H. Dental erosion and upper gastrointestinal disorders. Oral Surg Oral Med Oral Pathol 1988; 65: 298-303.
9. Bartlett DW, Coward PY. Comparison of the erosive potential of gastric juice and a carbonated drink in vitro. J Oral Rehabil 2001; 28: 1045-7.
10. Harley K. Tooth wear in the child and the youth. Br Dent J 1999; 186: 492-6.
11. Bartlett, DW, Ewans DF, Smith BGN. The relationship between gastroesophageal reflux disease and dental erosion. J Oral Rehabil 1996; 23: 289-97.
12. Jones L, Lekkas D, Hunt D, McIntyre J, Rafir W. Studies on dental erosion: An in vivo - in vitro model of endogenous dental erosion- its application to testing protection by fluoride gel application. Aust Dent J 2002; 38: 304-8.
13. Moazzez R, Bartlett DW, Anggiansah A. Dental erosion, gastro-esophageal reflux disease and saliva: how are they related? J Dent 2004; 32: 489- 94.
14. Lupi-Pegurier L, Muller M, Leforestier E, Bertrand MF, Bolla M. In vitro action of bordeux red wine on the microhardness of human dental enamel. Arch Oral Biol 2003: 48; 141-5.
15. Jain P, Nihill P, Sobkowski J, Agustin MZ. Commercial soft drinks: pH and in vitro dissolution of enamel. Gen Dent 2007; 55: 150-4.
16. Owens BM, Kitchens M. The erosive potential of soft drinks on enamel surface substrate: an in vitro scaning electron microscopy investigation. J Contemp Dent Pract 2007; 8: 11-20.
17. Hall AF, Buchanan CA, Millett DT, Creanor SL, Strang R, Foye RH. The effect of saliva on enamel and dentine erosion. J Dent 1999; 27: 333-9.
18. Willershausen B, Schulz-Dobrick B. In vitro study on dental erosion provoked by various beverages using electron probe microanalysis. Eur J Med Res 2004; 9: 432-8.
19. Phelan J, Rees J. The erosive potential of some herbal teas. J Dent 2003; 31: 241-6.
20. Pretty IA, Edgar WM, Higham SM. The validation of quantitative light- induced fluorescence to quantify acid erosion of human enamel. Arch Oral Biol 2004; 49: 285-94.
21. Chuenarrom C, Benjakul P. Comparison between a profilometer and a measuring microscope for measurment of dental enamel erosion. J Oral Sci 2008; 50: 475-9.
22. Myklebost P, Mosseng O-E, Gjerdet NR. Roughness of filling materials subjected to simulated gastric juice. J Dent Res 2003; 82(Spec Iss B): 378.
23. Eccles JD. Dental erosion of nonindustrial orijin: A clinical survey and classification. J Prosthet Dent 1979; 42: 649-53.
24. ten Bruggen Cate HJ. Dental erosion in industry. Br J Ind Med 1968; 25: 249-66.
25. Levine RS. Fruit juice erosion: An increasing danger? J Dent 1974; 70: 942-7.
26. Eccles JD, Jenkins WG. Dental erosion and diet. J Dent 1974; 2: 153-9.
27. Asher C, Read MJF. Early enamel erosion in children associated with the excessive consumption of citric acid. Br Dent J 1987; 162: 384-7.
28. Howden GF. Erosion as the presenting symptom in hiatus hernia. Br Dent J 1971; 131: 455- 6.
29. Hellström I. Oral complications in anorexia nevrosa. Scand J Dent Res 1977; 85: 71-86.
30. Clark C. Oral complications of anorexia nevrosa and/or blumia: With a review of the literature. J Oral Med 1985; 40: 134-8.
31. DeMeester TR, Johnson LF, Joseph GJ, Toscano MS, Hall AW, Skinner DB. Patterns of gastroeosophageal reflux in health and disease. Ann Surg 1979; 184: 459-70.
32. Oginni OA, Agbakwuru EA, Ndububa DA. The prevelance of dental erosion in Nigerian patients with gastroeosophageal reflux disease. BMC Oral Health 2005; 5: 1-6.
33. Dawes C. What is the critical pH and why does a tooth dissolve in acid? J Can Dent Assoc 2003; 69: 722-4.
34. Meurman, J. Toksala, J., Nuutinen P., Klemetti E. Oral and dental manifestations in gastroesophageal reflux diesease. Oral Surg Oral Med Oral Pathol 1994; 78: 583-9.
35. Schroeder PL, Filler SJ, Ramirez B, Lazarchik DA, Vaezi MF, Richter JE. Dental erosion and acid reflux disease. Ann Int Med 1995; 122: 809-15.
36. Holbrook WP, Furuholm J, Gudmundsson K, Theodórs A, Meurman JH. Gastric reflux is a significant causative factor of tooth erosion. J Dent Res 2009; 88: 422-6.
37. Di Fede O, Di Liberto C, Occhipinti G,Vigneri S, Lo Russo L, Fedele S,Lo Muzio L, Campisi G. Oral manifestations in patients with gastro-oesophageal reflux disease: a single-center case-control study.J Oral Pathol Med 2008; 37: 336- 40.
38. Milosevic A. Gastro-oesophageal reflux and dental erosion. Evid Based Dent 2008; 9: 54.
39. Cengiz S, Cengiz MI, and Saraç YS. Dental erosion caused by gastroesophageal reflux disease: a case report. Cases J 2009; 2: 8018.
40. Frank Spear. A Patient with severe wear on the anterior teeth and minimal wear on the posterior teeth. J Am Dent Assoc 2008; 139: 1399-403.
41. Machado C, Lacefield W, Catledge A. Human enamel nanohardness, elastic modulus and surface integrity after beverage contact. Braz Dent J 2008; 19: 68-72.
42. Kawai K, Urano M. Adherence of plaque components to different restorative materials. Oper Dent 2001; 26: 396-400.
43. Mandikos MN, McGivney GP, Davis E, Bush PJ, Carter JM. A comparison of the wear resistance and hardness of indirect composite resins. J Prosthet Dent 2001; 85: 386-95.
44. Tjan AH, Chan CA. The polishibility of posterior composites. J Prosthet Dent 1989; 61: 138- 46.
45. Jones CS, Billington RW, Pearson GJ. The in vivo perception of roughness of restoration. Br Dent J 2004; 196: 42-5.
46. Yap AU, Yap SH, Teo CK, Ng JJ. Finishing/polishing of composite and compomer restoratives: effectiveness of one-step systems. Oper Dent 2004; 29: 275-9.
47. West NX, Maxwell A, Hughes JA, Parker DM, Newcombe RG, Addy M. A method to measure clinical erosion: effect of orange juice consumption on erosion of enamel. J Dent 1998; 26: 329-35.
48. Ehlen LA, Marshall TA, Qian F, Wefel JS, Warren JJ. Acidic beverages increase the risk of in vitro tooth erosion. Nutr Res 2008; 28: 299-303.
49. Amaechi BT, Higham SM, Edgar WM. Factors influencing the development of dental erosion in vitro: enamel type, temperature and exposure time. J Oral Rehabil 1999; 26: 624-30.
50. Lussi A, Jaeggi T, Zero D. The role of diet in the aetiology of dental erosion. Caries Res 2004; 38: 34-44.
51. Larsen MJ, Nyvad B. Enamel erosion by some soft drinks and orange juices relative to their pH, buffering effect and contents of calcium phosphate. Caries Res 1999; 33: 81-7.
52. Davis RE, Marshall TA, Qian F, Warren JJ, Wefel JS. In vitro protection against dental erosion afforded by commercially available, calciumfortified 100 percent juices. J Am Dent Assoc 2007; 138: 1593-8.
53. Chadwick BL, White DA, Morris AJ, Evans D, Pitts NB. Non-carious tooth conditions in children in the UK, 2003. Br Dent J 2006; 200: 379- 84.