Ticari yumurtacı tavuklarda Escherichia coli O157:H7 varlığının araştırılması

Amaç: Bu çalışmada klinik yönden sağlıklı görünen ticari yumurtacı tavuk işletmelerinde bulunan tavuklardan toplanan kloakal sıvap örneklerinde Escherichia coli (E. coli) O157:H7 varlığının araştırılması amaçlandı. Gereç ve Yöntem: Konya ili ve çevresinde bulunan 10 farklı kümesteki yumurtacı tavuklardan 200 kloakal sıvap örneği toplandı. Tüm örnekler bakteriyolojik kültür yöntemi ile de- ğerlendirildi. İzolatların verotoksin üretimi lateral flow assay kiti ile belirlendi. Bulgular: Toplam 200 kloakal sıvap örneğinden 19 (%9.5) sorbitol negatif E. coli izole edildi. İzolatlardan 3 (%1.5)ü E. coli O157 olarak identifiye edildi ve bu suşlardan 1 (%0.5)i E. coli O157:H7 pozitif olarak bulundu. İzole edilen E. coli O157:H7 suşunun verotoksin 1 ve 2yi ürettiği belirlendi. Öneriler: Yumurtacı tavuk kloakal sıvap örneklerinden E. coli O157 ve E. coli O157:H7 belirlendiği dikkate alındığında veteriner halk sağlığı açısından önemli olabileceği ifade edilebilir

Investigation of the presence of Escherichia coli O157:H7 in commercial layers.

Aim: The purpose of this study was to investigate the presence of Escherichia coli (E. coli) O157:H7 strain in cloacal swab samples of clinically healthy commercial layers. Materials and Methods: A total of 200 cloacal swab samples were collected from laying hens at 10 different layer flocks in Konya province. All samples were examined by bacteriological culture method. Verotoxin productions of isolates were detected by lateral flow assay kit. Results: A total of 19 sorbitol negative E. coli strains were isolated from 19 (9.5%) of 200 cloacal swap samples. Three (1.5%) of the isolates were identified as E. coli O157 and one (0.5%) of them was found E. coli O157:H7 positive. The production of verotoxin 1 and 2 was detected in E. coli O157:H7 isolate. Conclusion: It may be stated that the occurrence of E. coli O157 and E. coli O157:H7 in cloacal swap samples of layers may be important in veterinary and human public health.

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