The role of dietary bee pollen in antioxidant potential in rats

Amaç: Mevcut araştırmada ratların oksidatif durum parametreleri üzerine gıda desteği olaral arı poleni ilavesinin etkisi incelendi.Gereç ve Yöntem: Yetişkin Wistar ratlar 3 gruba ayrıldı: Birinci grup kontrol (C) olarak ayrılırken, diğer iki grup deneme grupları (E1, E2) olarak ayrıldı. Deneme gruplarından E1 grubu diyetine 300 mg/kg arı poleni ilavesi yapılırken, ikinci E2 grubuna 500 mg/kg dozunda ilave yapılarak 90 gün beslendi. Arı poleni ilavesi yapılmayan grup ise kontrol grubu olarak değerlendirildi. Albümin, bilirubin, demir, total antioksidan durum ve süperoksid dismutaz düzeyleri spektrofotometri ile belirlendi.Bulgular: Ratların diyetlerine 500 mg/kg dozunda arı poleni ilavesinin albümin ve total antioksidan durum düzeylerinin istatistiki olarak (P

Rat diyetlerine arı poleni ilavesinin antioksidan durum üzerine etkisi

Aim: The present study was designed to determinate the effect of bee pollen as a feed additive on the selected parameters of antioxidant status of rats.Material and Methods: Adult Wistar rats were randomly divided into 3 groups: One control group (C) and two experimental groups (E1, E2). Experimental groups received dietary inclusion of collected bee pollen at 300 mg/kg in E1 group and 500 mg/kg in E2 group for 90 days. The group received feed without bee pollen addition served as the control group. Contents of albumin, bilirubin, iron, and total antioxidant status and superoxide dismutase activity were determined with spectrophotometer.Results: Supplementation of the diet with bee pollen in the dose of 500 mg/kg significantly (P

___

  • Ahn MR, Kunimasa K, Kumazawa S, Nakayama T, Kaji K, Uto Y, Hori H, Nagasawa H, Ohta T, 2009. Correlation betwe- en antiangiogenic activity and antioxidant activity of va- rious components from propolis. Mol Nutr Food Res, 53, 643-651.
  • Akkol EK, Orhan DD, Gürbüz I, Yesilada E, 2010. In vivo acti- vity assessment of a “honey-bee pollen mix” formulation. Pharm Biol, 48, 253-259.
  • Aliyazicioglu Y, Deger O, Ovali E, Barlak Y, Hosver I, Tekelioglu Y, Karahan SC, 2005. Effects of Turkish pollen and propolis extracts on respiratory burst for K-562 cell lines. Internati- onal Immunopharmacol, 5, 1652-1657.
  • Attia Y A, Al-Hanoun A, Bovera F, 2011a. Effect of different levels of bee pollen on performance and blood profile of New Zealand White bucks and growth performance of the- ir offspring during summer and winter months. J Anim Physiol Anim Nutr (Berl), 95, 17-26.
  • Attia YA, Al-Hanoun A, El-Din AE, Bovera F, Shewika YE, 2011b. Effect of bee pollen levels on productive, reproduc- tive and blood traits of NZW rabbits. J Anim Physiol Anim Nutr (Berl), 95, 294-303.
  • Bankova V, 2009. Chemical diversity of propolis makes it a va- luable source of new biologically active compounds. J ApiP- roduct ApiMed Sci, 1, 23-28. DOI: 10.3896/IBRA.4.01.2.01
  • Bell RR, Thormber EJ, Seet JLL, Groves MT, Ho NP, Bell DT, 1983. Composition and protein quality of honeybee col- lected pollen of Eucalyptus marginate and Eucalyptus ca- lophylla. J Nutr, 113, 2479-2484.
  • Bonvehí SJ, Torrentó SM, Lorente CE, 2001. Evaluation of polyphenolic and flavonoid compounds in honeybee- collected pollen produced in Spain. J Agr Food Chem, 49, 1848-1853.
  • Buck AC, Cox R, Rees RW, Ebeling L, John A, 1990. Treat- ment of outflow tract obstruction due to benign prosta- tic hyperplasi with the pollen extract, cernilton. A double- blind, placebo-controlled study. Brit J Urol, 66, 398-404.
  • Capcarova M, Slamecka J, Abbas K, Kolesarova A, Kalafova A, Valent M, Filipejova T, Chrastinova L, Ondruska L, Massan- yi P, 2011. Effects of dietary inclusion of Rhus coriaria on internal milieu of rabbits. J Anim Physiol Anim Nutr (Berl), 96, 459-465.
  • Echigo T, Yanami K, 1986. Studies on chemical composition of pollen loads. Honeybee Sci, 7, 97-100.
  • Eraslan G, Kanbur M, Silici S, 2009a. Effect of carbaryl on some biochemical changes in rats: the ameliorative effect of bee pollen. Food Cem Toxicol, 47, 86-91.
  • Eraslan G, Kanbur M, Silici S, Liman BC, Altmordulu S, Sari- ca ZS, 2009b. Evaluation of protective effect of bee pol- len against propoxur toxicity in rat. Ecotox Environ Safe, 72, 931-937.
  • Fatrcova-Sramkova K, Nozkova J, Mariassyova M, Kacaniova M, Dudrikova E, 2008. Content of polyphenols and antira- dical activity of bee pollen. Chem Listy, 102, 265-267.
  • Fu YY, Kang KJ, Ahn JM, Kim HR, Na KY, Chae DW, Kim S, Chin HJ, 2010. Hyperbilirubinemia reduces the streptozotocin- induced pancreatic damage through attenuating the oxida- tive stress in the Gunn rat. Tohoku J Exp Med, 222, 265-273.
  • Ishikawa Y, Tokura T, Ushio H, Niyonsaba F, Yamamoto Y, Ta- dokoro T, Ogawa H, Okumura K, 2009. Lipid-soluble com- ponents of honeybee-collected pollen exert antiallergic ef- fect by inhibiting IgE-mediated mast cell activation in vivo. Phytoth Res, 23, 1581-1586.
  • Izuta H, Narahara Y, Shimazawa M, Mishima S, Kondo S, Hara H, 2009. 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of bee products and their constituents determined by ESR. Biol Pharm Bull, 32, 1947-1951.
  • Jackie T, Haleagrahara N, Chakravarthi S, 2011. Antioxi- dant effects of Etlingera elatior flower extract against lead acetate-induced perturbations in free radical scavenging enzymes and lipid peroxidation in rats. BMC Res Notes, 4, doi:10.1186/1756-0500-4-67.
  • Kacaniova M, Fatrcova-Sramkova K, Nozkova J, Melich M, Kadasi-Horakova M, Knazovicka V, Felsöciova S, Kunova S, Mariassyova M, 2011. Antiradical activity of natural ho- neys and antifungal effect against Penicillium genera. J En- viron Sci Health, Part B, 46, 92-96.
  • Kanner J, Frankel E, Granit R, German B, Kinsella JE, 1994. Natural antioxidants in grapes and wines. J Agr Food Chem, 42, 64-69.
  • Leja M, Mareczek A, Wyzgolik G, Klepacz-Baniak J, Czekonska K, 2007. Antioxidative properties of bee pollen in selected plant species. Food Chem, 100, 237-240.
  • Marinova MD, Tchorbanov BP, 2010. Preparation of antio- xidant enzymatic hydrolysates from honeybee-collected pollen using plant enzymes. Enzyme Res, ID 415949, doi:10.4061/2010/415949
  • Martin-Munoz MF, Bartolome B, Caminoa M, Bobolea I, Ara MC, Quirce S, 2010. Bee pollen: a dangerous food for aller- gic children. Identification of responsible allergens. Aller- gol Immunopathol, 38, 263-265.
  • Maruyama H, Sakamoto T, Araki Y, Hara H, 2010. Anti- inflammatory effect of bee pollen ethanol extract from Cistus sp. of Spanish on carrageenan-induced rat hind paw edema. BMC Complement Altern Med, 10, 30, doi: 10.1186/1472-6882-10-30.
  • Missima F, Pagliarone AC, Orsatti CL, Sforcin JM, 2009. The ef- fect of propolis on pro-inflammatory cytokines produced by melanoma-bearing mice submitted to chronic stress. J ApiProduct ApiMed Sci, 1, 11-15.
  • Moreira LL, Dias LG, Pereira JA, Estevinho L, 2008. Antioxi- dant properties, total phenols and pollen analysis of pro- polis samples from Portugal. Food Chem Toxicol, 46, 3482-3485.
  • Münstedt K, Bogdanov S, 2009. Bee products and their po- tential use in modern medicine. J ApiProduct ApiMed Sci, 1, 57-63.
  • Naik DG, Vaidya HS, Behera BC, 2009. Antioxidant properti- es of Indian propolis. J ApiProduct ApiMed Sci, 1, 110-120.
  • Nakajima Y, Tsuruma K, Shimazawa M, Mishima S, Hidea- ki H, 2009. Comparison of bee products based on assays of antioxidant capacities. BMC Complemen Altern Med, 9, doi:10.1186/1472-6882-9-4. http://www.biomedcentral. com/1472-6882/9/4
  • Petruska P, Tusimova E, Kalafova A, Hascik P, Kolesarova A, Capcarova M, 2012. Effect of propolis in chicken diet on se- lected parameters of mineral profile. J Microbiol Biotech- nol Food Sci, 1, 593-600.
  • Saric A, Balog T, Sobocanes S, Kusic B, Sverko V, Rusak G, Li- kic S, Bubalo D, Pinto B, Reali D, Marotti T, 2009. Antioxi- dant effects of flavonoid from Croatian Cystus incanus L. rich bee pollen. Food Chem Toxicol, 47, 547-554.
  • Smith MW, Shenoy KB, Debnam ES, Dashwood MR, Churchill LJ, Srai SK, 2002. Divalent metal inhibition of non-haem uptake across the rat duodenal brush border membrane. Br J Nutr, 8, 51-56.
  • Srigiridhar K, Nair KM, 2000. Supplementation with alpha- tocopherol or a combination of alpha-tocopherol and as- corbic acid protects the gastrointestinal tract of iron- deficient rats against iron-induced oxidative damage du- ring iron repletion. Br J Nutr, 84, 165-173.
  • Stohs SJ, Bagchi D, 1995. Oxidative mechanisms in the toxi- city of metal ions. Free Radical Biol Med, 18, 321-336.
  • Stocker R, 2004. Antioxidant activities of bile pigments. Anti- oxid Redox Signal, 6, 841-849.
  • Valko M, Morris H, Cronin MTD, 2005. Metals, toxicity and oxidative stress. Curr Med Chem, 12, 1161-1208.
  • Vinson JA, Hontz BA, 1995. Phenol antioxidant index: compa- rative antioxidant effectiveness of red and white wines. J Agr Food Chem, 43, 401-403.
  • Xu X, Sun L, Dong J, Zhang H, 2009. Breaking the cells of rape bee pollen and consecutive extraction of functional oil with supercritical carbon oxide. Innov Food Sci Emerg Technol, 10, 42-46.
Eurasian Journal of Veterinary Sciences-Cover
  • ISSN: 1309-6958
  • Yayın Aralığı: Yılda 4 Sayı
  • Yayıncı: Selçuk Üniversitesi Veteriner Fakültesi
Sayıdaki Diğer Makaleler

Retrospective study of calf mortality on Central Cattle Breeding and Dairy Farm (CCBDF) in Bangladesh

Mukter HOSSAİN, Abu Hena Mustafa KAMAL, AKM Anisur RAHMAN

Süt inekleri için hazırlanan kombine mastitis aşılarının farelerde etkinliğinin belirlenmesi

Dursun Ali DİNÇ, Hülya TÜRÜTOĞLU, Zafer SAYIN, H. Hüseyin HADİMLİ, Osman ERGANİŞ, Kürşat KAV

Nerium oleander distilatının antimikrobiyal etkisinin araştırılması

Burak DİK, Orhan ÇORUM, Zafer SAYIN

Evaluation of the quality characteristics of fermented sausages and sausage-like products sold in Kars

Aksem AKSOY, Leyla VATANSEVER, Turgay DEPREM, Metin ÖĞÜN, Çiğdem SEZER, Nebahat BİLGE ORAL, Abamüslüm GÜVEN, Özgür ÇELEBİ

The prevalence of helminth infections in water buffaloes in the vicinity of Afyonkarahisar province

Esma KOZAN, Hasan GÜZEL

Microbiological and chemical qualities of cream-filled pastries sold in Kerman city confectioneries, southeast of Iran

Masoud SAMI, Mahboobeh BAGHERI, Hamid SHARIFI, Atefeh NASRI

Serological and virological investigation of Bovine Viral Diarrhea Virus infection in cattle with abortion problem

Atilla ŞİMŞEK, Sibel YAVRU, Oya BULUT, Oğuzhan AVCI, Orhan YAPICI

General overview to treatment of strong ion (metabolic) acidosis in neonatal calves with diarrhea

İsmail ŞEN, Peter D. CONSTABLE

Subklinik mastitisli süt ineklerinden izole edilen katalaz negatif Gram pozitif kokların identifikasyonu ve antibiyotiklere duyarlılıkları

H. Hüseyin HAMDİMLİ, Zafer SAYIN, Osman ERGANİŞ, Kürşat KAV

The role of dietary bee pollen in antioxidant potential in rats

Marcela CAPCAROVA, Adriana KOLESAROVA, Anna KALAFOVA, Branislav GALİK, Milan SİMKO, Miroslav JURACEK, Robert TOMAN