Tuğba ÖZAYDIN, Emrah SUR, Yasemin OZNURLU, İlhami ÇELİK

165

Histological Examination Of The Skin And AgNOR Parameters Of Matrix Pili Cells İn The Chinchilla

Çinçilyalarda derinin histolojisi ve kıl matriks pili hücrelerinde AgNOR sayısının ışık mikroskobik seviyede incelenmesi amaçlandı. Gereç ve Yöntem: Onbeş adet ergin ve sağlıklı çinçilyadan alınan deri örnekleri tamponlu formol salin solüsyonunda tespit edildi ve rutin histolojik yöntemlerle parafinde bloklandı. Bloklardan alınan kesitler Crossmon’un üçlü boyaması, Gordon ve Sweet’in retiküler iplik boyaması, Verhoef’in elastik iplik boyaması ve AgNOR boyama yöntemleriyle boyandı. Derinin toplam kalınlığı ile epidermis ve dermis kalınlıkları, birim alandaki (1 mm2) ortalama kıl folikül sayıları ve germinal matriks pili hücrelerinde AgNOR sayısı ve alanı belirlendi. Bulgular: Germinal matriks hücrelerinin çekirdeklerindeki AgNOR’ların kahverengi-siyah lekeler halinde 1 ile 4 adet arasında değişen sayılarda olduğu tespit edildi. Çinçilyalarda birleşik kıl foliküllerinin bir ya da iki primer folikül çevresinde çok sayıda sekonder folikülden oluştuğu gözlendi. Sekonder foliküllerin primer foliküllere oranı (S/P) 13/1 olarak tespit edildi. Öneri: Daha geniş populasyonlarda ve mevsimsel değişiklikler göz önüne alınarak yapılacak çalışmalarda, kıl foliküllerinin sayı ve dağılımı ile germinal matriks hücrelerindeki AgNOR parametrelerinin belirlenmesi seleksiyonda kürk kalitesi için önemli bir kriter olabilir.

In this study, histology of the skin and number and area of the argyrophilic nucleolar organiser regions (AgNORs) of the germinal matrix epithelial cells of the chinchilla were investigated by light microscopic techniques. Materials and Methods: Skin samples were taken from 15 adult healthy chinchilla, fixed in formol saline and immersed in parafine. Tissue sections were stained with Crossman’s trichrome, Gordon and Sweet’s reticuler fiber, Verhoef’s elastic fibre and AgNOR staining methods. The total thickness of epidermis and dermis, hair follicle numbers per square milimetres, and number and area of AgNORs of the germinal matrix epithelial cells were measured. Results: AgNORs were seen as 1-4 black dots which distributed in the nuclei of the germinal matrix cells of the hair follicles. The chinchilla compound hair follicle consisted of one or two primary follicles surrounded by multiple smaller secondary follicles. The ratio of secondary to primary hair follicles (S/P) was 13/1. Conclusion: In further studies on a larger population seasonal differences, determination of number and distribution of hair follicules and AgNOR parameters in the germinal matrix cell should be used as significant criteria for fur quality in selection.
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Eurasian Journal of Veterinary Sciences-Cover
  • ISSN: 1309-6958
  • Yayın Aralığı: Yılda 4 Sayı
  • Yayıncı: Selçuk Üniversitesi Veteriner Fakültesi
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Histological Examination Of The Skin And AgNOR Parameters Of Matrix Pili Cells İn The Chinchilla

Tuğba ÖZAYDIN, Emrah SUR, Yasemin OZNURLU, İlhami ÇELİK