Beta Glucosidase Recognition By Imprinted Polyacrylamide Hydrogels
Beta Glucosidase Recognition By Imprinted Polyacrylamide Hydrogels
In the present work, selective adsorption of β-glucosidase using imprinted polyacrylamide hydrogels were studied. For this purpose imprinted hydrogels were prepared using β-glucosidase as a template molecule, acrylamide (AAm) as a monomer, N,N’- methylenebisacrylamide (MBAA) as a crosslinker, ammonium persulphate (APS) and N,N,N’,N’-tetramethylethylene-diamine (TEMED) as initiators. β-Glucosidase imprinted hydrogel was washed with a solution of sodium dodecyl sulfate (SDS) and acetic acid to remove the template molecule. Non-imprinted hydrogel was also prepared without using β-glucosidase template. The adsorption and recognition performance of hydrogels towards β-glucosidase was discussed through adsorption isotherms and adsorption kinetics. In batch template rebinding experiments, imprinted hydrogels displayed quite high template binding capacity than non-imprinted hydrogels. The theoretical maximum adsorption capacity (Qmax) was determined by the Langmuir model, which turned out to be 7.2 mg/g and 4.6 mg/g for imprinted and non-imprinted hydrogels respectively. A pseudo-second-order model was suitable to interpret kinetic data.
Keywords:
β-Glucosidase, polyacrylamide, hydrogel,
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- Yayın Aralığı: Yılda 2 Sayı
- Başlangıç: 2018
- Yayıncı: Muhammet DOĞAN
Sayıdaki Diğer Makaleler
Kübra UĞUR, Muhammet DOĞAN, Abdullah KAYA
Erol ATAY, Levent EFİL, Mahmut TATLI, Berrin ALACA
Ayşen AKTÜRK, Büşra CENİK, Zeliha AYDOĞDU, Melek EROL TAYGUN, Funda KARBANCIOĞLU GÜLER, Sadriye KÜÇÜKBAYRAK
Meliha KOLDEMİR GÜNDÜZ, Mehtap ÇEVİK, Penbe ÇAĞATAY, Belgin SÜSLEYİCİ
Beta Glucosidase Recognition By Imprinted Polyacrylamide Hydrogels