Türkiye Genelinde Sığır Paratüberkülozu Prevalansının Elisa ile Araştırılması
Sığır paratüberkülozu (PTB) dünyada yaygın , yetiştiricilik endüstrisinde büyük ekonomik kayıplara yol
açan, kronik seyirli bir hastalıktır. Ülkemizde bugüne kadar PTB ' nin prevalansının saptanmasına yönelik genel
bir çalışma yapılmamıştır. Bu nedenle hastalığın ülkemizde oluşturduğu kayıplarda bilinmemektedir. Bu
çalışmada Türkiye coğrafi özelliklerine göre 9 bölgeye bölünmüştür. Bu bölgelerdeki 2 yaş ve üzeri sığırlardan
basit tesadüfi örnekleme ile 8873 kan serum örneği toplanmıştır. Örnekler absorbe ELISA ile test edilmiştir.
Örneklerin 409'u pozitif bulunmuştur. ELISA ' nın sensitivitesi %44, spesifitesi %96 olarak tespit edilmiştir. Bu
değerlere göre bölgelerdeki gerçek bireysel prevalans belirlenmiştir. Sonuçlar 1. bölgeden başlamak üzere
sırasıyla %2.2 , %3.6, %3.7, %3.6, %0, %1.2, %9.6, %4.5 ve %4.4.olarak bulunmuştur. Türkiye genelinde
gerçek bireysel prevalans %3.7 olarak tespit edilmiştir. Ayrıca bu çalışmada her köy bir sürü olarak kabul
edilmiş, buna göre görünen sürü prevalansı belirlenmiştir. Sonuçlar 1. bölgeden başlamak üzere sırasıyla %33.3,
%43.6, %27.2, %43. 1 , %26.6, %38.0, %48.2, %31.8 ve %41.2 olarak saptanmışt ır. Sürüleri n %1 7.2'sinde
pozitif hayvan, %20.5 ' inde en az 2 hayvan pozitif bulunmuştur.
Prevalance of Paratuberculosis in the Turkish Cattle Population by Using Absorbed Elisa
Bovine paratuberculosis, widespread in the world, is a chronicle disease which causes a lot of
economical losses in the agricultural industry. In our country there has been no study on estimating the
prevalance of PTB so far. For this reason it is not known what losses the disease has caused in Turkey. In this
study Turkey was dividcd into 9 provinces in accordance with the geographical characteristics. 8873 sera
samples from the callle that were at least 2 years old were collected randomly in these provinces. The saınples
were tested with absoı·bed ELISA , 409 of samples were positive. The sensitivity and the specificity of the
ELISA were 44% and 96% respectively. The true individual - animal prevalance in each province was
determined as regards to the sensitivity and specificity ınentioned above . The results, starting from the first
province was 2.2%, 3.6%, 3.7%, 3.6%, 0%, 1 .2%, 9.6%, 4.5% and 4.4% respectively. The average true
individual- aniınal prevalance in Turkey was found 3.7%. In this study each viiiage was assume as a herd and
the apparent herd prevalance was determined . The results , starting from the first province was 33.3%, 43.6%,
37.2%, 43.1 %, 26.6%, 38.0%, 48.2%, 31.8% and 41.2% respectively. The herds were divided into two groups
one of which had 1 positive and theothersat least 2 positive cattle. 17.2% of the herds had one positive cattle
and 20.5% of the herds had at least 2 positive cattle.
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- ALİBAŞOGLU M, ERTÜRK E, YÜCEL N
(1973). Türkiyede rastlanan ilk keçi
paratüberküloz olayları üzerinde patolojik
incelemeler. A Ü Vet Fak Derg, XX, 1, 43-
63.
- ARDA M, MİNBA Y A, LELOGLU N,
AYDIN N, AKAY Ö. (1992). Özel
Mikrobiyoloji. Atatürk Üniv Yay , 741 ,
279-3 311.
- BOELAERT F, WALRA VENSK , BIRONT
P, VERMEERSCH J P, BERKVENS D ,
GODFROID J (2000). Prevalence of
paratuberculosis (Johne's disease) in the
Belgian cattle population.,Vet Microbio/.77 ,
269-281.
- CAMPHAUSEN R T , JONES R L ,
ERENNAN P J (1988). Antigenic
relationship between Mycobacterium
paratuberculosis and Mycobacterium avium.
Am] Vet Res49,8,1307-1310.
- COLGROVE G S , THOEN C O ,
BLACKBURN B O , MURPHY C D
( 1989). Paratuberculosis in cattLe: a
comparison of three seralogic tests with
results of fecal culture. Vet Microbiol 19, 2,
183-187.
- COX J C , DRANE D P , JONES S L ,
RIDGE S , MILNER A R (1991).
Development and evaluation of a rapid
absorbed enzy;,ıe immunoassay test for the
diagnosis of Johne's disease in cattle. Aust
Vet J, 68,157-160.
- ÇETINKAYA B , ERDOGAN H M ,
MORGAN K L (1997).Mycobacterium
paratuberculosis infections: classification,
pathogenesis, İmmunology and relationship
with Crohn 's disease. Veterinarium, 8, 1-
2,89-98.
- ÇETINKAYA B , ERDOGAN H M ,
MORGAN K L (1998).Prevalence,
ineidence and geographical distribution of
Johne 's disease in cattle in England and the
Welsh borders. Vet Rec,143,265-269 .
- DANIEL TM and DEBANNE S M (1987).
The seradiagnosis of tuberculosis and other
Mycobacteriel diseases by Enzyme-Linked
Immunosorbent Assay. Anı Rev Respir Dis
135,1137-1151.
- DEMİRER F , YÜCEL N (1964).Çifteler
Haraası ile Alpu 'daki Mesut Zeytinoğluna
ait çiftlikteki sığırların gaitasından
M.paratuberculosis (Johne's) ın memeleketimizde ilk İzolasyonu. Türk Vet
Hek Derg, 34,3-4,170-171.
- DIMECH W (2000). Automation of an
absorbed enzyın immunoassay for the
detection of Mycobacterium
paratuberculosis antibodies for an
eradication program. Vet Microbiol, 77,35 1-
355.
- ELLIS TM, CARSON BA , KALKHOVEN
MJ , MARTIN PAJ (1998).Specifity of two
absorbed ELISA's for surveys of
Mycobacterium paratuberculosis in cattle.
,Aust Vet 1,76,497-499.
- EMRE MN (1967). Yurdumuz sığırlarında
paratüberkülozun teşhis metodlarıüzerinde
mukayeseli atraştırmalar ve değişik
M.paratüberküloz suşlarının bazı
antiseptiklere karşı hassasiyetlerinin tayini.
Güven Matbaast , ANKARA.
- GRIFFITH AS, TYTLER WH, CUMMIS
SL, MCINTOS J, WHITBY LEH,
BILLOCH W , FLEMING A , OKELL
CC, GLOYNE SR ( 1930). Johne's
diseases. In: A System of Bacteriology ın
Relation to Medicine. Majesty's Station e1y
Office, London, p.: 333--344.
- JACOBE HB, CARRIGAN M,
COCKRAM F, CORNER LA, GILL IJ,
HILL JF , JESSEP T , MILNER AR,
WOOD PR (1992).A comparison of the
interferon gamma assay with the absorbed
ELISA for the diagnosis of Johne 's disease
in cattle. Aust Vet 1. 69,25-28.
- MARCHEVSKY N (1974). Errors ın
prevalence estimates in population studies:
A practical method for calculating real
prevalence. Zoonosis. 16, 98- 109.
- MERKAL R S (1984). Paratuberculosis:
Advances ın cultural, serologic, and
vaccination methods. lA VMA , 184, 8, 939-
943.
- MILNER AR, MACK WN, COATES KJ,
HILL J, GILL I, SHELDRICK P (1990).
The sensitivity and specificity of a ınodifıed
ELISA for the diagnosis of Johne's disease
from a field trial in cattle. Vet
Microbiol.25 , 193-198.
- MILNER A R , MACK W N , COATES K ,
WOOD P R SHELDRICK P , HILL J ,
GILL I (1989).The absorbed ELISA for the
diagnosis of Johne's disease in cattle. Vet Bul
158-163.
- OIE (1996). World Organisation for
Animal Health, Manuel of standards for
diagnostic tests and vaccines, 267-275.
- ÖKTEM Z (1967). Mycobacterium. İç .:
Tıbbi Bakteriyoloji. IL Cild, Menleş
Kitabevi, İstanbul, p.: 604-673.
- REICHEL MP, KıTTELBERGER R,
PENROSE ME, MEYNELL RM, COUSINS
D, ELLIS T, MUTHARIA LM, SUGDEN
EA, JOHNS AH, DE LISLE GW (1999).
Comparison of serological tests and faecal
culture for the detection of Mycobacterium
avium subsp. paratuberculosis infection in
cattle and analysis of the antigens involved.
Vet Microbiol, 66, 135- 150 .
- RIDGE SE, MORGAN IR, SOCKETT DC,
COLLINS MT, CONDRON RJ,
SKILBECK NW, WEB BER JJ (1991 ).
Comparison of the Johne's absorbed EIA
and the complement-fixation test for the
diagnosis of Johne' s disease in cattle . Aust
Vet 1, 68 ,253-257.
- RINGDAL G (1963). Johne's disease in
pigs. Nord Vet Med, 15 , 217-238.
- RINGDAL G (1965). Studies on Johne's
disease in a single herd during a few year
period. Nord Vet Med, 17 , 73-96.
- SCHAIK G, KALIS CHJ, BENEDICTUS
G, DIJKHUIZEN AA, HUIRNE RBM
( 1996). Cost-benefit analysis of vaccination
against paratuberculosis in dairy cattle. Vet
Rec 139,624-627.
- SEZGİNER FR (1928). Ehli hayvanlarda
intani hastalıklar. Hilal Matbaası, İstanbul,
252-259.
- SOCKETT DC (2000). Johne's disease
diagnosis and control. Wisconsin Animal
Health Lab.-Madison,
- STABLEFORTH AV (1961). Thecontrol of
Johne' s disease. Tijdschr(f V oor
Diergeneeskunde 86 E Deel AFL, 25, 1744-
1750.
- SWEENEY RW, WHITLOCK RH,
ROSENBERGER AE (1992). Mycobacteriuın
paratuberc ulosis isolated from
foetuses of infected cows not manifesting
signs of the di sease. Am J Vet Res,53,4,477-
480.
- SWEENEY RW, WHITLOCK RH, HAMIR
AN, ROSENBERGER AE, HERR SA
( 1992).Isolation of Mycobacterium
paratuberculosis after oral inoculation in
uninfected cattle. Am J Vet Res, 53,8 ,1312-
1314.
- WAYNE LG , KUBICA GP ( 1986).
The Mycobacteria. In: Bergey's Manuel
of Determinative Bacteriology. Williams
& Wilkins, Baltimore, p.: 1435-1457.
- WENTING GH, BONGERS JH, VOS JH,
ZEEUWEN AA PA (1993). Relationship
between negative skin test with Johnin after
vaccination and post mortem finding. Vet
Rec, 132,38-39.
- WILLIAMS ES, DEMARTİNİ JC,
SNYDER SP ( 1985). Lymphocyte
blastogenesis, complement fixation , and
faecal culture as diagnostic tests for
paratuberculosis ın North American wild
ruminants and domestic sheep. Am J Vet
Res, 46,11 ,2317-2321.
- YOKOMIZO Y ( 1986). Evaluation of an
enzym-linked immunosorbent as say
(ELISA) usıng Mycobacterium phleiabsorbed
serum for the diagnos is of bovine
paratuberculosis in a field study. Jpn Agric
Res,20, 59-67.
- YOKOMIZO Y, YUGI H and MERKAL
RS (1985). A method for avoiding falsepozitive
reactions ın an enzym-linked imunosorbent assay (ELISA) for the
diagnosis of bovine paratuberculosis. Jpn J
VetSci,47, 111-119.