Mycoplasma gallisepticum ve Mycoplasma synoviae teşhisi için real-time PCR yöntemi geliştirilmesi

Kanatlı mikoplazmaları solunum yolu hastalığı, sinovitis, günlük civcivlerin kalitesizliği ve düşük performans ile ilişkilidir. Kanatlı mikoplazmalarının teşhisinde kullanılan temel yaklaşım mikroorganizmanın izolasyonu ve identifikasyonudur. Mikoplazma yavaş ve zor üreyen organizmalar olduğundan, konvansiyonel yöntemler zaman alıcı, zahmetli ve deneyimli personel gerektirir. Bu nedenle, kantitatif gerçek zamanlı polimeraz zincir reaksiyonu (qPCR) ile Mycoplasma gallisepticum (MG) ve Mycoplasma synoviae (MS) için hızlı bir tespit yöntemi geliştirmeyi amaçladık. Bu amaçla, sırasıyla lipoprotein (lp) ve değişken lipoprotein hemaglütinin (vlhA) genleri M. gallisepticum ve M. synoviae’nın teşhisi için kullanıldı. Yapay kontaminasyon yapılan svap örneklerinde, geliştirilen metodun deteksiyon limiti (LOD)

Development of real-time PCR method for the diagnosis of Mycoplasma gallisepticum and Mycoplasma synoviae

Avian mycoplasmas are associated with respiratory disease, synovitis, poor quality of day-old chicks, andpoor performance. The main approach used for the diagnosis of avian mycoplasmas is isolation and identification ofthe microorganism. Since the Mycoplasma are slow-growing fastidious organisms, conventional methods are timeconsuming, laborious, and require experienced personnel. For this reason, we aimed to develop a rapid detectionmethod for Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) by quantitative real-time polymerasechain reaction (qPCR). For this purpose, the lipoprotein (lp) and variable lipoprotein hemagglutinin (vlhA) geneswere used to detect M. gallisepticum and M. synoviae, respectively. The limit of detection (LOD) of the assay wasdetermined to be

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