Elçin GÜNAYDIN, Asiye DAKMAN, Uğur KÜÇÜKAYAN, H. Kaan MÜŞTAK

Atık fetus mide içeriklerinden konvansiyonel kültürel yöntem ve polimeraz zincir reaksiyonu ile Brucella spp.’nin teşhisi

Brusellosis, Brucella türlerinin neden olduğu oldukça bulaşıcı, zoonotik bir enfeksiyondur. Brusellozisin teşhisinde, serolojik ve konvansiyonel kültürel yöntemlerin yanı sıra farklı polimeraz zincir reaksiyonu (PZR) uygulamaları da kullanılmaktadır. Bu çalışmada 31 adet sığır, 8 adet koyun ve 1 adet keçi fötal atık mide içeriğinden bakteriyolojik kültür ve PZR ile Brucella spp.’nin belirlenmesi ve her iki teşhis yönteminin karşılaştırılması amaçlanmıştır. Çalışma sonunda her iki yöntemle de benzer pozitif ve negatif sonuçlar elde edilmiştir.

Anahtar Kelimeler:

Brucella spp., fetus, konvansiyonel kültürel yöntem, mide içeriği, PZR, Asiye DAKMAN, Uğur KÜÇÜKAYAN, Elçin GÜNAYDIN, H. Kaan MÜŞTAK, Etlik Veteriner Mikrobiyoloji Dergisi

Diagnosis of Brucella spp. by conventional cultural method and polymerase chain reaction in stomach contents of aborted fetuses

Brucellosis is a contagious, zoonotic infection caused by Brucella species. Beside the serological and conventional cultural methods, different polymerase chain reaction (PCR) applications can be used in diagnosis of brucellosis. The aim of this study was to compare bacteriological culture and PCR methods used for the detection of Brucella spp. in stomach contents of the 31 bovine, 8 ovine and 1 caprine aborted fetuses. At the end of the study, same positive and negative results were obtained from both methods.

Keywords:

Brucella spp., conventional cultural method, fetus, PCR, stomach content,

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