S. İsmet GÜRHAN, Burhan GÜRHAN, Aysun ÖZTÜRKMEN, Gülhan AYNAGÖZ, Aysel CANDAŞ, Serdar KIZIL

Anadolu'da Şap ile Persiste Enfekte Sığır ve Koyunların Prevalansının Tesbiti

Şap virusu ile persiste enfekte hayvanların prevalansını tesbit etmek amacı ile Anadolu'da 16 ilden 469 koyun ve 147 sığır probang numunesi toplantı. Primer dana tiroid (BYT) ve IB-RS 2 devamlı hücre kültürlerinde gerçekleştirilen izolasyon ve CFT ve/veya ELISA ile gerçekleştirilen identifikasyon işlemleri sonucunda taşıyıcılık oranı koyunlar için %61,8 ve sığırlar için de %18,4 olarak belirlendi. Orta ve Güneydoğu Anadolu'daki taşıyıcı hayvan sayısı batı ve kuzey bölgelerine göre daha fazla idi. 4 koyunun hem A hem de O tipi şap virusu taşıyıcısı olduğu tesbit edildi. BTY hücrelerinin IB-RS 2 devamlı hücre kültürlerine oranla şap virusunun saha suşlarına daha duyarlı olduğu konusundaki önceki bulgular teyid edildi. Bununla beraber, BTY hücre kültürlerinin dezavantajları ve şap viruslarının hücre kültürlerinde enteroviruslar tarafından maskeleme olasılığı göz önüne alınarak yeni ve daha duyarlı test sistemlerinin laboratuvarlara adaptasyonu önerildi.

Anahtar Kelimeler:

Şap, Şap Virusu, Sığırlarda Şap, Persiste Enfeksiyon, S. İsmet GÜRHAN, Burhan GÜRHAN, Aysun ÖZTÜRKMEN, Gülhan AYNAGÖZ, Aysel CANDAŞ, Serdar KIZIL

«Establishment of the prevalance of persistently infected cows and sheep in Anatolia with FMDV»

For the purpose of the determination of the prevalance of the animals persistentiy infected with FMDV, probang samples of 469 sheep and 147 cattle fomı 16 provinces in different districts of Anatolia have been collected. At the end of the isolation carried out with primary bovine thyroid (BTY) cell cultures and IB-RS 2 eell-line cultures and identification by CFT and/or ELISA the carrier rate in sheep population has been detemıined 16.8 %and in cattle population 18.4% . The number of carrier animalsin Middle-East and South-East Anatolia was more then the westren and northem parts. 4 sheep have been detennined both A and O type FMDV carriers. It has been. confınned that BTY cells were more susceptible to FMDV field samples than IB-RS2 cell-1ine. Neverthe1ess, by taking both the disadvantages of BTY cell culture system and the possible masking of FMDV partides by enteroviruses into consideration it has been recommended to search and adapt other susceptible system to the laboratory.

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