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Tubifex tubifex'DE TALYUM İLE İNDÜKLENEN OKSİDATİF STRESE KARŞI OLUŞAN ANTİOKSİDAN YANITLAR

Talyum (Tl)’un canlı sistemler üzerindeki etkisi son yıllarda önemli bir sorun haline gelmiştir. Bununla birlikte, sucul sistemlerin Tl’a maruz kalmasının sonuçları ile ilgili çok az bilgi mevcuttur. Bu çalışmanın amacı, sucul oligoket Tubifex tubifex’in antioksidan biyolojik belirteçlerinde (metallotionein (MT), toplam glutatyon (GSH), glutatyon peroksidaz (GPx), glutatyon S-transferaz (GST) ve katalaz (CAT)), TI’un subletal dozlarına yanıt olarak meydana gelen değişikliklerin araştırılmasıdır. Deney grupları 0.25, 0.5 ve 1 µg l-1’lik konsantrasyonlarda, 7 ve 15 günlük süreler boyunca talyum asetat’a maruz bırakılmıştır. MT seviyelerinde ilgi çekici bir azalma gözlenmiştir. 0.25 µg l-1’ lık konsantrasyon toplam GSH, GPx ve GST aktiviteleri üzerinde etki göstermezken, 0.5 ve 1 µg l-1’ lık konsantrasyonlar toplam GSH ve GPx aktivitelerini 7 günün sonunda indüklemiştir. Daha uzun maruz kalma süresi GPx ve GST aktiviteleri ile birlikte toplam GSH’un da tüm konsantrasyonlarda azalmasına neden olmuştur. CAT aktivitesinde tüm deney gruplarında gözlenen anlamlı artışa rağmen, lipid peroksidasyonunun (LP) artan konsantrasyon ve süreye bağlı olarak arttığı gözlenmiştir. Sonuçlar Tl ile indüklenen oksidatif strese karşı, T. tubifex’in antioksidan sisteminin olası hareket mekanizmasını ortaya koymakta ve Tl’un sucul ekosistemlerdeki etkileri ile ilgili gelecekte yapılacak değerlendirmeler için önemli bir bilgi kaynağı oluşturmaktadır.
Anahtar Kelimeler:

Talyum, Tubifex tubifex, Oksidatif stres, Antioksidanlar, Lipit peroksidasyonu

ANTIOXIDATIVE RESPONSES IN Tubifex tubifex AGAINST THALLIUM INDUCED OXIDATIVE STRESS

Thallium (Tl) impact on living systems has become an important issue in recent years. However very few data exist about the consequences of Tl exposure in freshwater aquatic systems. The aim of the present study is to investigate alterations in the levels of antioxidative biomarkers (metallothionein (MT), total glutathione (GSH), glutathione peroxidase (GPx), glutathione S-transferase (GST) and catalase (CAT)) in the aquatic oligochaete Tubifex tubifex, in response to sublethal doses of Tl.  Experimental groups were exposed to thallium acetate at 0.25, 0.5 and 1 µg l-1 concentrations during 7 or 15 days. MT levels were observed to be interestingly decreased. 0.25 µg l-1 concentration had no effect on total GSH, GPx and GST activities while 0.5 and 1 µg l-1 concentrations induced total GSH and GPx activitiy after 7 days. Longer exposure time resulted in a decrease in total GSH along with GPx and GST activities at all concentrations of Tl. Despite the significant elevation of CAT activity in all experimental groups, lipid peroxidation (LP) was observed to be induced depending on the increasing exposure concentration and time. The results suggested the possible mechanism of action in the antioxidant system of T. tubifex in response to Tl induced oxidative stress (OS) and provide useful information for the future evaluation of Tl impact on aquatic ecosystems.
Keywords:

Thallium, Tubifex tubfex, Oxidative stres, Antioxidants, Lipid peroxidation,

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Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji-Cover
  • ISSN: 2667-4203
  • Yayın Aralığı: Yılda 2 Sayı
  • Başlangıç: 2010
  • Yayıncı: Eskişehir Teknik Üniversitesi
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Tubifex tubifex'DE TALYUM İLE İNDÜKLENEN OKSİDATİF STRESE KARŞI OLUŞAN ANTİOKSİDAN YANITLAR

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