A BIO-BASED RHEOLOGY MODIFYING AGENT INSPIRED FROM NATURE
Interaction of microorganisms and building materials, particularly with
concrete and stone, were a main topic of interest for many researchers.
Initially, studies focused on degradation of concrete by
organic acids, produced by microbial acidification such as microbial induced
corrosion. This was followed by prevention of microorganisms fouling on building
materials. However, the interaction of microorganisms with materials is not
necessarily negative. Recent research in the field shows that microorganisms
can have positive effects on concrete and stone, such as in biological cleaning
and biocalcification, which resulted with stronger and more durable materials.
Further, studies revealed that it was possible to develop smart-cement based
materials that could self-heal microorganisms by leveraging metabolic activity
of microorganisms. Through the development of this so-called smart bio-based
mortar, it became possible to improve the fresh state performance of the mix.
This study focusses on design of a cement-based mortar with improved
rheological properties with use of Bacillus
megaterium (B. megaterium) and
Bacillus subtilis (B. subtilis) cells. The bacterial cells were directly
incorporated to the mix water and influence of cells on viscosity and yield
strength was evaluated by rheological tests. In addition, the influence of
bacteria dosage, water to cement ratio (w/c), use of superplasticizers and fly
ash on performance of biological VMA were investigated. Our results showed that
the apparent viscosity and yield stress of the cement-paste mix were increased
with the addition of the microorganisms. Moreover, B. megaterium cells were found to be compatible with both fly ash
and superplasticizers however B. subtilis
were only be able to increase the viscosity when they were incorporated with
superplasticizers.
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- [1] Achal V, Pan X, Özyurt N Improved strength and durability of fly ash-amended concrete by microbial calcite precipitation. Ecol Eng, 2011; 37:554–559. doi: 10.1016/j.ecoleng.2010.11.009
- [2] Andalib R, Abd Majid MZ, Hussin MW, Ponraj M, Keyvanfar A, Mirza J, Lee HS Optimum concentration of Bacillus megaterium for strengthening structural concrete. Constr Build Mater 2016; 118:180–193. doi: 10.1016/j.conbuildmat.2016.04.142
- [3] ASTM C305 Standard Practice for Mechanical Mixing of Hydraulic Cement Pastes and Mortars of Plastic Consistency. ASTM lnternational, 2011; 1–3. doi: 10.1520/C0305-13.2
- [4] Basaran Bundur Z, Kirisits MJ, Ferron RD. Biomineralized cement-based materials: Impact of inoculating vegetative bacterial cells on hydration and strength. Cem Concr Res, 2015; 67:237–245. doi: 10.1016/j.cemconres.2014.10.002
- [5] Esmaeilkhanian B, Feys D, Khayat KH, Yahia A. New test method to evaluate dynamic stability of self-consolidating concrete. ACI Mater J, 2014; 111:299–307. doi: 10.14359/51686573
- [6] Feys D, Verhoeven R, De Schutter G Fresh self compacting concrete, a shear thickening material. Cem Concr Res, 2008; 38:920–929. doi: 10.1016/j.cemconres.2008.02.008
- [7] G Ozcelik, AP Gürsel ÇM Yap ı Kimyasallar ı na Çevresel Çerçeveden Bak ış. In: Uluslararası Katılımlı Yapılarda Kimyasal Katkılar 4. Sempozyumu ve Sergisi, 2013; pp 179–197
- [8] Ivanov V, Chu J, Stabnikov V. Basics of Construction Microbial Biotechnology. In: Pacheco-Torgal F, Labrincha JA, Diamanti MV, Yu C (eds) Biotechnologies and Biomimetics for Civil Engineering. Springer, 2015; pp 21–56
- [9] Jiménez M, Mateo R, Mateo JJ, Huerta T, Hernández E. Effect of the incubation conditions on the production of patulin by Penicillium griseofulvum isolated from wheat. Mycopathologia, 1991;115:163–168. doi: 10.1007/BF00462220
- [10] Khayat KH Viscosity-enhancing admixtures for cement-based materials — An overview. Cem Concr Compos, 1998; 20:171–188. doi: 10.1016/S0958-9465(98)80006-1
- [11] Kurdowski W. Cement and Concrete Chemistry, 2014; Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7945-7
- [12] Lachemi M, Hossain KMA, Lambros V, Nkinamubanzi PC, Bouzoubaâ N. Self-consolidating concrete incorporating new viscosity modifying admixtures. Cem Concr Res, 2004; 34:917–926. doi: 10.1016/j.cemconres.2003.10.024
- [13] Pei R, Liu J, Wang S. Use of bacterial cell walls as a viscosity-modifying admixture of concrete. Cem Concr Compos, 2015; 55:186–195. doi: 10.1016/j.cemconcomp.2014.08.007
- [14] Plank J. Applications of biopolymers and other biotechnological products in building materials. Appl Microbiol Biotechnol, 2004; 66:1–9. doi: 10.1007/s00253-004-1714-3
- [15] Rafaï S, Jibuti L, Peyla P. Effective Viscosity of Microswimmer Suspensions. Phys Rev Lett, 2010; 104:1–4. doi: 10.1103/PhysRevLett.104.098102
- [16] Saintillan D Kinetic models for biologically active suspensions. 2012; Urbana, ill.
- [17] Schmidt W, Brouwers HJH, Kühne HC, Meng B The working mechanism of starch and diutan gum in cementitious and limestone dispersions in presence of polycarboxylate ether superplasticizers. Appl Rheol, 2013; 23:1–12. doi: 10.3933/ApplRheol-23-52903
- [18] Sonebi M Rheological properties of grouts with viscosity modifying agents as diutan gum and welan gum incorporating pulverised fly ash. Cem Concr Res, 2006; 36:1609–1618. doi: 10.1016/j.cemconres.2006.05.016
- [19] Vary PS, Biedendieck R, Fuerch T, Meinhardt F, Rohde M, Deckwer WD, Jahn D Bacillus megaterium-from simple soil bacterium to industrial protein production host. Appl Microbiol Biotechnol, 2007; 76:957–967. doi: 10.1007/s00253-007-1089-3
- ISSN: 2667-4211
- Yayın Aralığı: Yılda 4 Sayı
- Başlangıç: 2000
- Yayıncı: Eskişehir Teknik Üniversitesi