GREEN SYNTHESIS OF SILVER NANOPARTICLES AS AN ANTIBACTERIAL AGENT: OPTIMIZATION OF SYNTHESIS CONDITIONS WITH RESPONSE SURFACE METHODOLOGY
In the present work, the synthesis of silver nanoparticles (AgNPs) through a green synthesis method using avocado (Persea americana) leaf as a biological reductant and capping agent. The synthesized AgNPs were characterized by zetasizer, FTIR, UV-vis spectrophotometer, XRD, and SEM. Besides, response surface methodology was used to understand and optimize the effect of the experimental parameters on the green synthesis of AgNPs. Three experimental parameters were chosen as independent variables: temperature, AgNO3 concentration, and aqueous leaf extract volume. A quadratic model was established as a functional relationship between three independent variables and the effective hydrodynamic diameter (nm) of AgNPs. The results of model fitting and statistical analysis demonstrated that only AgNO3 concentration was statistically significant parameter. The optimum conditions for minimum effective hydrodynamic diameter (nm) of AgNPs (32.74 nm) were temperature of 25 oC, AgNO3 concentration of 0.001 M, and aqueous leaf extract volume of 50 mL. Moreover, the antibacterial activities of AgNPs for S. typhimurium, E. coli O157:H7, S. aureus, and L. monocytogenes were tested and it was found that AgNPs had stronger inhibitory effects on growth of L. monocytogenes than the other bacteria.
Keywords:
antibacterial activity, avocado leaf, optimization, response surface methodology, silver nanoparticles,
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- Mehmood A, Murtaza G, Bhatti TM, Raffi M, Kausar R. Antibacterial efficacy of silver nanoparticles synthesized by a green method using bark extract of Melia azedarach L. J Pharm Innov 2014; 9(3): 238-245.
- Umer A, Naveed S, Ramzan N, and Rafique MS. Selection of a suitable method for the synthesis of copper nanoparticles. Nano 2012; 7(05): 1230005.
- Ahmad A, Wei Y, Syed F, Tahir K, Rehman AU, Khan A, Yuan Q. The effects of bacteria-nanoparticles interface on the antibacterial activity of green synthesized silver nanoparticles. Microb Pathog 2017; 102: 133-142.
- Sun L, Wan S, Yu Z, and Wang L. Optimization and modeling of preparation conditions of TiO2 nanoparticles coated on hollow glass microspheres using response surface methodology. Sep Purif Technol 2014; 125: 156-162.
- Awwad AM, Salem NM, Abdeen AO, Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity. IJIC 2013; 4(1): 1-6.
- Özer A, Gürbüz G, Çalimli A, Körbahti BK. Biosorption of copper (II) ions on Enteromorpha prolifera: application of response surface methodology (RSM). Chem Eng J 2009; 146(3): 377-387.
- Gade A, Gaikwad S, Duran N, and Rai M. Green synthesis of silver nanoparticles by Phoma glomerata. Micron 2014; 59: 52-59.
- Khalil MM, Ismail EH, El-Baghdady KZ, and Mohamed D. Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arab J Chem 2014; 7(6): 1131-1139.
- Fakhri A. Investigation of mercury (II) adsorption from aqueous solution onto copper oxide nanoparticles: optimization using response surface methodology. Process Saf Environ 2015; 93: 1-8.
- Kathiraven T, Sundaramanickam A, Shanmugam N, and Balasubramanian T. Green synthesis of silver nanoparticles using marine algae Caulerpa racemosa and their antibacterial activity against some human pathogens. Appl Nanosci 2015; 5(4): 499-504.
- Muthukumaran U, Govindarajan M, Rajeswary M, Hoti SL. Synthesis and characterization of silver nanoparticles using Gmelina asiatica leaf extract against filariasis, dengue, and malaria vector mosquitoes. Parasitol Res 2015; 114(5): 1817-1827.
- Zhong Q, and Jin M. Zein nanoparticles produced by liquid–liquid dispersion. Food Hydrocoll 2009; 23(8): 2380-2387.
- ISSN: 2667-4211
- Yayın Aralığı: Yılda 4 Sayı
- Başlangıç: 2000
- Yayıncı: Eskişehir Teknik Üniversitesi