Tek Kullanımlık Kalem Grafit Elektrot Kullanılarak Rutinin Voltametrik Tayini

Bu çalışmada, rutinin tayini için elektrokimyasal bir yöntem geliştirildi. Kalem grafit elektrot tek kullanımlık ve düşük maliyetli bir elektrottur. Kalem grafit elektrot, camsı karbon elektrota kıyasla pik akımının önemli ölçüde artmasıyla çok iyi bir katalitik etki göstermiştir. Optimize edilmiş koşullar altında, kalem grafit elektrot, 0.104 ile 166.70×10-7 M ve 166.70 ile 1060.60×10-7 M rutin olmak üzere iki doğrusal yanıta sahiptir, Tespit ve tayin limitleri sırasıyla 1.13×10-9 M (S / N = 3) ve 3.48×10-9 M olarak hesaplandı. Yüzde geri kazanım, kabul edilebilir tekrarlanabilirliği gösteren, ardışık beş tekrarlı rutin analizi için % 98.98 ile % 101.02 arasında bir aralıkta elde edilmiştir. Geliştirilen yöntem; karabuğday, yeşil çay ve kırmızı elma gibi gerçek numunelerde, rutinin doğrudan tayini için başarıyla uygulanmıştır. Son olarak, bazı türlerin rutin tayinine girişim etkileri de değerlendirilmiştir.

Anahtar Kelimeler:

Flavonoid, Rutin, Kalem grafit elektrot, Voltametrik tayin

Voltammetric Determination of Rutin by Using Disposable Pencil Graphite Electrode

In this study, an electrochemical method was developed for the determination of rutin. The pencil graphite electrode, a disposable and low cost electrode, showed a very good catalytic effect with the significant enhancement of the peak current compared to the glassy carbon electrode. Under optimized conditions, the pencil graphite electrode had two linear ranges from 0.39 to 166.70×10-7 M and 166.70 to 1060.60×10-7 M rutin, the limit of detection was also obtained 1.13 nM (S/N=3). The percentage of recoveries were obtained in a range between 98.98 and 101.02 % for five successive determinations of rutin, which indicate acceptable repeatability. This method was successfully applied for the direct determination of rutin in real samples such as buckwheat, green tea and red apple. Finally, the interference effects of some species to the determination of rutin was also evaluated.

Keywords:

Flavonoid, Rutin, Pencil graphite electrode, Voltammetric determination,

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