Esen SEZEN KARAOĞLAN, Bilal YILMAZ

Determination of β-Sitosterol in Alchemilla caucasica Buser by GC-MS Method

Bu çalışmada Alchemilla caucasica bitkisinde β-sitosterol’ün gaz kromatografisi-kütle spektrometri yöntemiyle belirlenmesi için bir yöntem geliştirmesi amaçlandı. β-sitosterol için, seçilmiş iyon görüntüleme ve elektron iyonizasyon modları kullanıldı. Geliştirilen yöntemin doğrusal aralığı β-sitosterol için 1-100 μg/mL olarak elde edildi. β-sitosterol için gün-içi ve günler-arası kesinlik değerinin %4.99'dan daha düşüktü. Yöntemin doğruluğunun %4.60'dan daha iyi olduğu görüldü. Gözlenebilme ve miktar tayin sınırları β-sitosterol için sırasıyla 0.05 ve 0.15 μg/mL olarak bulundu. A. caucasica bitkisinde β-sitosterol miktarı 2.26%’lık geri kazanımıyla 22.6 μg/1.0 mg olarak bulundu. Öyle ki bu yöntem, A. caucasica bitkisinde β-sitosterol’ün tayini için de kullanılabilir.

Anahtar Kelimeler:

Alchemilla caucasica, β-sitosterol, GC-MS, Validation

Determination of β-Sitosterol in Alchemilla caucasica Buser by GC-MS Method

In this study, it was aimed to develop a method for the determination of β-sitosterol by gas chromatography-mass spectrometry method in Alchemilla caucasica Buser. For β-sitosterol, selected ion monitoring and electron ionization modes were used. Calibration linearity of the devoloped method was obtained at 1-100 μg/mL concentration for β-sitosterol. The intra-day and inter-day precision value for β-sitosterol was less than 4.99%. The accuracy of the method was observed to be better than 4.60%. The detection and quantification limits for β-sitosterol were found 0.05 and 0.15 μg/mL, respectively. The amount of β-sitosterol in A. caucasica plant was found to be 22.6 μg/1.0 mg with percent recovery 2.26% for β-sitosterol. Also, this method can be used for the determination of β-sitosterol in A. caucasica plant.

Keywords:

Alchemilla caucasica, β-sitosterol, GC-MS, Validation,

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