Balıkesir İli’nden Toplanan Geranium Macrorrhizum (Geraniaceae) Türünün Metanol Ekstresinin Biyolojik ve Antimikrobiyal Aktivitesinin Belirlenmesi

Dünyanın değişik bölgelerinde Geranium türleri, ağrı kesici, kan dindirici, antihemoroidal, antidiyabetik, ateşdüşürücü, antidiyareik, kan temizleyici ve gastrointestinal sistem rahatsızlıklarının tedavisinde kullanılmaktadır.Bu çalışma da Balıkesir ili’nden toplanan Geranium macrorrhizum türünün metanol ekstresinde biyolojik veantimikrobiyal aktivite çalışmaları yapılmıştır.“Geranium macrorrhizum metanol ekstresinde”, numunenin toplam fenolik içerik miktarı 130.18 µg GAE / 1gkuru örnek olarak bulunmuştur. Geranium macrorrhizum metanol ekstresinde, toplam flavonoid içerik miktarı55,56 ± 1,20 mg QE/g kuru numune olarak belirlenmiştir. Geranium macrorrhizum (Geraniaceae)’nın metanolekstresinde ABTS radikal giderme aktivitesi 5.80 ± 0,55 lC50 (μg/mL) olarak bulunmuştur. DPPH radikaliolarak antioksidan aktivitesi 10,05 μg/mL olarak belirlenmiştir. Geranium macrorrhizum metanol ekstresi içinen duyarlı bakterinin Klebsiella pneumoniae ATCC700603 (13 mm) olduğu tespit edilmiştir. Elde edilenbulgular Geranium macrorrhizum bitkisinin tıbbi bitki olarak değerlendirilebileceğini göstermektedir.İleri araştırmalarda G. macrorrhizum’un içerdiği kimyasal bileşenlerin belirlenmesinin yanı sıra in situ ve invivo testler ile etki mekanizmalarının daha detaylı araştırılmasının gerekli olduğu sonucuna varılmıştır

Determination of Biological and Antimicrobial Activity of Methanol Extract of Geranium Macrorrhizum (Geraniaceae) Species Collected from Balıkesir Province

Geranium species are used in various regions of the world to treat pain relief, blood-relieving, antihemoroidal, antidiabetic, antipyretic, antidiarrheal, blood-purifying and gastrointestinal system disorders. In this study, biological and antimicrobial activity studies were performed on the methanol extract of Geranium macrorrhizum species collected from Balıkesir province. In the Geranium macrorrhizum methanol extract, the total phenolic content of the sample was found to be 130.18 in GAE / 1g dry sample. In the geranium macrorrhizum extract, the total amount of flavonoid content was determined as 55.56 ± 1.20 mg QE / g dry sample. ABTS radical removal activity in the methanol extract of Geranium macrorrhizum (Geraniaceae) was 5.80 ± 0.55 lC50 (μg / mL). Antioxidant activities as DPPH radicals were determined as 10,05 μg / mL. The most sensitive bacterium for Geranium macrorrhizum extract was Klebsiella pneumoniae ATCC700603 (13 mm). The results show that Geranium macrorrhizum can be considered as a medicinal plant. The chemical compounds in G. macrorrhizum as well as in situ and in vivo tests and the mechanisms of action should be investigated in more detail in further investigations.

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