Gülşah SAYDAN KANBEROĞLU, Erol ŞENER, Muzaffer TUNCEL, Zühre ŞENTÜRK

Akış-Enjeksiyon Analiz Tekniği ile UV Dedektör Kullanılarak İlaçlarda Asemetasin Tayini

Bu çalışmada romatizma tedavisinde kullanılan antiinflamatuvar-analjezik etkili ilaçlardan Asemetasin bileşiğinin doğrudan tayininde UV-dedektörlü akış-enjeksiyon analiz (FIA) tekniği tanımlanmıştır. En uygun taşıyıcı çözücünün, etanol:su (20:80, h/h) içerisinde hazırlanan 2 mM NaOH olduğu saptanmıştır. Akış hızı olarak 1,2 mL dak-1 ve çalışma dalga boyu olarak 280,8 nm değerleri kullanıldığında Asemetasin için 1,2×10-5‒5,8×10-5 M (5,0‒24,1 μg mL-1)derişim aralığında, 1,6×10-6 M (0,7 μg mL-1) gözlenebilirlik sınırına sahip doğrusal bir aralık elde edilmiştir. Önerilen yöntem, bu bileşiği içeren ticari kapsül ilaç şekline başarıyla uygulanmış ve sonuçlar bu çalışmada geliştirilen basit bir UV-spektrofotometrik yöntemle karşılaştırılmıştır.

Anahtar Kelimeler:

Asemetasin, akışa enjeksiyon analizi, UV dedektör, miktar tayini

Determination of Asemetacin in Drugs by Flow-Injection Analysis Technique Using UV Detector

In this study, a flow-injection analysis (FIA) system with UV-detection is described for the direct determination of Acemetacin, which is antiinflammatory-analgesic drug used for the treatment of rheumatic diseases. The best suitable carrier solvent system was found to be 2 mM NaOH prepared in ethanol :water (20:80, v/v). Using a flow-rate of 1,2 mL min-1 and wavelength of 280,8 nm, the linear response was obtained for Acemetacin in the range of 1,2×10-5‒5,8×10-5 M (5,0‒24,1 μg mL-1)with a detection limit of 1,6×10-6 M (0,7 μg mL-1). The proposed method was successfully applied to the determination of Acemetacin in commercial capsules and results were compared by a simple UV-spectrophotometric method developed in this study.

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