Ratlarda İndometazinle Oluşturulan Gastrik Ülser Üzerine Silimarinin Koruyucu Etkileri

Doğal polifenolik yapılı flavonoid bir antioksidan olan silimarinin serbest radikallere ve reaktif oksijen türlerine karşı etkili olduğu bildirilmiştir. İndometazin, nonsteroidal anti-inflammatuar bir ilaç olup mide ülseri yapıcı etkileri bulunmaktadır. Bu çalışmada indometazin ile indüklenmiş mide ülseri modelinde silimarinin koruyucu etkinliği araştırıldı. Çalışma kapsamında 42 adet erkek Wistar albino sıçan kullanıldı ve sıçanlar kontrol, indometazin, lansoprazol+indometazin, silimarin (25, 50 ve 100 mg/kg)+indometazin olacak şekilde altı gruba ayrıldı (n=7). Farklı dozlarda silimarin ağızdan gavajla uygulandı ve 5 dk sonra 25 mg/kg indometazin ile gastik ülser indüklendi. Koruyucu etkinlik lansoprazol (30 mg/kg) ile karşılaştırıldı. Sıçanlar indometazin uygulamasından 6 saat sonra ötenazi edildi. Mide dokusu superoksid dismutaz (SOD), katalaz (CAT) ve miyeloperoksidaz (MPO) aktivitesi ile malondialdehid (MDA) ve glutatyon (GSH) seviyesi analiz edildi. Mide dokusu aynı zamanda makroskobik olarak ülseratif alanlar yönünden de değerlendirildi. Çalışma sonucunda 100 mg/kg dozda silimarinin anti-ülseratif etkisinin olduğu; bu dozda verilen silimarinin mide dokusu MDA seviyesi ve MPO aktivitesini anlamlı olarak azalttığı, SOD aktivitesi ve GSH seviyesini arttırdığı belirlendi. Sonuç olarak indometazin ile indüklenen mide ülseri ve oksidatif streste silimarinin koruyucu etkilerinin olabileceği kanaatine varıldı.

Anahtar Kelimeler:

İndometazin, mide ülseri, rat, silimarin

Protective Effects of Silymarin on Indomethacin-Induced Gastric Ulcer in Rats

Silymarin is a natural antioxidant polyphenolic flavonoid structure, it has been reported to be effective against free radicals and reactive oxygen species. Indomethacin is a nonsteroidal anti-inflammatory drug and can promote ulcer in gastric tissue. The purpose of this study was to investigate the protective effects of silymarin on indomethacin-induced gastric ulcer model. Forty two male Wistar albino rats were divided into 6 groups (n=7); control, indomethacin, lansoprazole+indomethacin, silymarin (25, 50 and 100 mg/kg)+indomethacin group. Different doses of silymarin were administered by oral gavage and then gastric ulcers were induced by oral administration of indomethacin (25 mg/kg). Ulcer protective activity of silymarin was compared with lansoprazole (30 mg/kg). The rats were euthanized 6 h after indomethacin exposure. Activities of superoxide dismutase (SOD), catalase (CAT) and myeloperoxidase (MPO) as well as malondialdehyde (MDA) and glutathione (GSH) levels were determined in stomach tissue. Gastric tissue was also assessed in terms of macroscopic as ulcerative areas. As a result, 100 mg/kg silymarin was showed antiulceratif effects. The same dose of silymarin significantly decreased MDA level and MPO activity, and increased SOD activity and GSH level in stomach tissue. Silymarin might have a protective effect against indomethacin-induced gastric ulcer as well as oxidative stress in rat.

Keywords:

Indomethacin, gastric ulcer, rat, silymarin,

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