Antibiotic Susceptibility of Motile Aeromonas spp. Isolated from Foods of Animal Origin and Water

In this study, presence of motile Aeromonas spp. in different foods of animal origin and water by cultural method and detection to antibiotic susceptibilities of the isolates were investigated. A total of 224 food samples such as fish, chicken, fishery products, milk, meat and water supplied from points of sale like market and bazaar in and around Izmir have been examined. Aeromonas selective agar was used for Aeromonas spp. isolation. Gram-negative and oxidase positive colonies were assessed in Vitek 2 system (France) for identification. Of 224 food samples (meat, chicken, fish, fishery products, milk and water) 87 have been found to be positive for motile Aeromonas spp. According to the study, 51 (22.76%) of them were determined as A. hydrophila, 26 (11.6%) of them as A. caviae and 10 (4.6%) of them as A. sobria. Antibiotic susceptibility of Aeromonas isolates was determined according to the Kirby Bauer disk diffusion method. All Aeromonas spp. isolates have been found to be susceptible to imipenem and ciprofloxacin.

Anahtar Kelimeler:

Antibacterial susceptibility, food, isolation, motile Aeromonas spp.

PDF

___

1. Awan BM, Maqbool A, Bari A, Krovacekek K. Antibiotic suscebility profile of Aeromonas spp. isolates from food in Abu Dhabi, United Arab Emirates. New Microbiol 2009; 32(1): 17-23. 2 Biomerieux, Vitek 2 Tecnology. Online software user manuel, 510773-3EN1, Marcy I’etoile, France, 05/2006. 3. Daskalov H. The importance of Aeromonas hydrophila in food safety. Food Control 2006; 17(6): 474-83. 4. Castro-Escarpulli G, Figueras MJ, Aguilera-Arreola G, Soler L, Fernandez-Rendon E, Aparicio GO, Guarro J, Chacon MR. Characterisation of Aeromonas sp. isolated from frozen fish intended for human consumption in Mexico. Int J Food Microbiol 2003; 84(1): 41-9. 5. Gobat PT, Jemmi T. Distrubition of mesophilic Aeromonas in raw and ready-to-eat fish and meat products in Switzerland. Int J Food Microbiol 1993; 20(2):117-20. 6. Gonzalez-Rodrigues MN, Santos JA, Otero A, Garcia-Lopez ML. PCR detection of potentially pathogenic Aeromonad in raw an cold-smoked freshwater fish. J Appl Microbiol 2002; 93(4): 675-80. 7. Neyts K, Huys G, Uytendaele M, Swings J, Debevere J. Incidence and identification of mesophilic Aeromonas spp from retail foods. Lett Appl Microbiol 2000; 31(5): 359-63. 8. Radu S, Ahmad N, Ling FH, Reezal A. Prevalence and resistance to antibiotics for Aeromonas species from retail fish in Malasia. Int J Food Microbiol 2003; 81(3): 261-6. 9. Santos Y, Toranzo AE, Barja JL, Nieto TP, Villa TG. Virulence properties and enterotoxsin production of Aeromonas strains isolated from fish. Infect Immun 1988; 56(12): 3285-93. 10. Sechi LA, Deriu A, Falchi MP, Fadda G, Zanetti S. Distribution of virulence genes in Aeromonas sp. isolated from Sardinian waters and from patients with diarrhoea. J Appl Microbiol 2002; 92(2): 221-7. 11. Seethalakshmi I, Subashkumar R, Saminanthan P. Distribution of putative virulance genes in Aeromonas hydrophila and Aeromonas salmonicida isolated from marketed fish samples. J Fish Aquat Sci 2008; 3(2) 145-51. 12. Stratev D, Odeyemi OA. Antimicrobial resistance of Aeromonas hydrophila isolated from different food sources: A mini-review. J Infect Public Health 2016; 9(5): 535-44. 13. Praveen K, Debnath H, Shekhar S, Dalai N, Ganguly S. Incidence of Aeromonas spp. infection in fish and chicken meat and its related public health hazard: A review. Vet World 2016; 9(1): 6-11. 14. Topçu S. Ankara’da satışa sunulan döner kebap çeşitlerinden L. monocytogenes S.aureus A. hydrophila izolasyonu ve çeşitli antibiyotik dirençlilikleri Yüksek Lisans Tezi, Gazi Ü. Fen Bilimleri Enstitüsü, Ankara 2003; p. 1-40.