Trichogramma embryophagum Hartig (Hymenoptera: Trichogrammatidae)’in ömür uzunluğu, parazitleme performansı ve verimliliği üzerine Wolbachia spp.’nin etkisi

Wolbachia cinsi endosimbiyontlar Trichogramma türlerinde yaygın olarak görülmektedir. Wolbachia spp. bazı Trichogramma türlerinde tam partenogenezi (telitoki) uyaran zorunlu hücre içi bakteridir. Telitoki sonucu döllenmemiş yumurtalardan sadece dişi Trichogramma’lar oluşmakta bu da biyolojik mücadele için bir avantaj sağlamaktadır. Bu çalışma parazitoitteki Wolbachia spp. kaynaklı bakteriyel enfeksiyonunu Wolbachia yüzey protein-PCR yöntemi kullanarak tanımlamak ve bu enfeksiyonun Ephestia kuehniella Zeller yumurtalarında yetiştirilen Trichogramma embryophagum Hartig’in ömür uzunluğu ve parazitleme performansı üzerindeki etkilerini belirlemek amacıyla yapılmıştır. Kontrol grubu olarak ısı ile muamele edilmiş hat (arhenotoki) kullanılmıştır. Wolbachia spp. T. embryophagum’un verimliliği üzerinde herhangi bir negatif etki göstermemiştir. Isı ile muamele edilmiş arıların sadece erkek döl ürettiği görülmüştür. Bu arıların, Wolbachia-enfekteli olanlara göre daha uzun yaşadığı ancak bu farklılığın istatistiksel açıdan önemli olmadığı görülmüştür.

Anahtar Kelimeler:

Trichogramma embryophagum, Wolbachia spp., Partenogenez, Ömür uzunluğu, telitoki

The effect of Wolbachia spp. on the longevity, parazitization performance and fecundity of Trichogramma embryophagum Hartig (Hymenoptera: Trichogrammatidae)

Endosymbionts of the genus Wolbachia are commonly found in Trichogramma species. Wolbachia spp. is obligatory intracellular bacteria that induced complete parthenogenesis (thelytoky) in some Trichogramma species. The result of Thelytoky, only Trichogramma females occurs from unfertilized eggs and this provides an advantage in biological control.. This study was conducted to define the Wolbachia induced bacterial infection in parasitoid using Wolbachia surface protein-PCR method and to determine the effects of bacterial infection on the longevity and parasitization performance of Trichogramma embryophagum Hartig reared on Ephestia kuehniella Zeller eggs as a host. Wolbachia spp. had not showed any negative effect on fecundity of T. embryophagum. Heat treated lines (arrhenotokous) were used as control group. Heat treated wasps produced only male progeny. These wasps survived longer than Wolbachia-infected ones, however this difference was not statistically significant.

Keywords:

Trichogramma embryophagum, Wolbachia spp., Parthenogenesis, Longevity, Thelytoky,

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