Fatih AĞDAŞ, Aylin ERYILMAZ, Esra Gökmen YILMAZ, Kemal ERGİN

The effects of Sulindac on cell viability, cell cycle and angiogenesis in pharyngeal cancer cells

Objective: The present study aimed to investigate the effects of Sulindac on cell viability, cell cycle and angiogenesis in pharyngeal cancer cell lines (FaDu).Methods: FaDu cells were incubated in a medium in a5% CO2 incubator at 37°C, after which they were proliferated and passaged. IC50 concentration was used todetermine the Sulindac dose. Cells were analyzed for cellviability, cell count and cell cycle after Sulindac administration. Immunohistochemistry (vascular endothelialgrowth factor receptor 2) and western blot (A disintegrinand metalloproteinase with thrombospondin-like motifs1-ADAMTS1) analyses were used for angiogenesis assessment.Results: Cell viability decreased in pharyngeal cancer cellsafter Sulindac administration. In addition, FaDU cellswere arrested in the G2/M phase. Sulindac was foundto slightly increase vascular endothelial growth factorreceptor 2 (VEGF-R2) and decrease ADAMTS1 levels inpharyngeal cancer cells.Conclusion: Sulindac showed positive results on cell proliferation in the treatment of pharyngeal cancer cells.However, it could cause a slight increase in angiogenesis.

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