Sitokin geni polimorfizmleri ve Türk pediyatrik koklear implant hastalarındaki ekspresyonu
Sitokin geni polimorfizmleri ve Türk pediyatrik koklear implant hastalarındaki ekspresyonu
Objective:We assessed the association between the polymorphisms and expressions of three cytokine genes and clinical parameters in children who underwent cochlear implantation due to profound congenital sensorineural hearing loss. Methods:We analyzed the IL-6/-174, IFN-γ/+874 and TNF-α/-308 genes in 64 cases with congenital sensorineural hearing loss and in 70 healthy controls. Cytokine genotyping/expression was performed using the PCR-SSP method. Results: No significant differences were detected between the patient group and the healthy controls with respect to the distributions and numbers of genotypes and alleles of TNF-or IL-6. However, the TT genotype, associated with high expression of IFN-γ, and the Tallele frequency were significantly more frequent in the patient group versus the controls (p=0.016 and 0.023, respectively). Conclusion: Our results suggest that high expression of the IFN-γ gene may be associated with susceptibility to the disease. Consequently, IFN-γ may be a useful marker of the etiopathogenesis.
___
- 1. Vivero RJ, Fan K, Angeli S, Balkany TJ, Liu XZ. Cochlear
implantation in common forms of genetic deafness. Int J Pediatr
Otorhinolaryngol 2010;74:1107–11.
- 2. Lalwani AK, Castelein CM. Cracking the auditory genetic code:
nonsyndromic hereditary hearing impairment. Am J Otol 1999;
20:115–32.
- 3. Pati S, Pinninti S, Novak Z, et al.; NIDCD CHIMES Study
Investigators. Genotypic diversity and mixed infection in newborn
disease and hearing loss in congenital cytomegalovirus
infection. Pediatr Infect Dis J 2013;32:1050–4.
- 4. Lindberg E, Andersson B, Eggertsen R, Nyström E, Magnusson
Y. A polymorphism at position +874 in the IFN-γ gene is associated
with susceptibility for dilated cardiomyopathy. J Clin Cell
Immunol 2010;1:101.
- 5. Alper CM, Winther B, Hendly JO, Doyle WJ. Cytokine polymorphisms
predict the frequency of otitis media as a complication
of rhinovirus and RSV infections in children. Eur Arch
Otorhinolaryngol 2009;266:199–205.
- 6. Online Mendelian Inheritance in Man. Interferon, Gamma; IFNG
[Internet]. Baltimore, MD: Johns Hopkins University [cited 2013
July 25] Available from: http://omim.org/entry/147570.
- 7. Gaur U, Aggarwal B. Regulation of proliferation, survival and apoptosis
by members of the TNF superfamily. Biochem Pharmacol
2003;66:403–8.
- 8. Vadlamani L, Iyengar S. Tumour necrosis factor α polymorphism
in heart failure/cardiomyopathy. Congest Heart Fail
2004;10:289–92.
- 9. Wilson AG, Symons JA, McDowel TL, McDevit HO, Duff
GW. Effects of a polymorphism in the human tumor necrosis
factor alpha promoter on transcriptional activation. Proc Natl
Acad Sci U S A 1997;94:3195–9.
- 10. Fonseca JE, Santos MJ, Canhao H, Choy E. Interleukin-6 as a key
player in systemic inflammation and joint destruction. Autoimmun
Rev 2009;8:538–42.
- 11. Fishman D, Faulds G, Jeffery R, et al. The effect of novel polymorphism
in the interleukin-6 (IL-6) gene on IL-6 transcription
and plasma IL-6 levels, and an association with systemic onset
juvenile chronic arthritis. J Clin Invest 1998;102:1369–76.
- 12. Schroder K, Hertzog PJ, Ravasi T, Hume DA. Interferongamma:
an overview of signals, mechanisms and functions. J
Leukoc Biol 2004;75:163–89.
- 13. Pravica V, Asderakis A, Perry C, et al. In vitro production of
IFN-γ correlates with CA repeat polymorphisms in the human
IFN-γ gene. Eur J Immunogenet 1999;26:1–3.
- 14. Col-Araz N, Pehlivan S, Baspinar O, Oguzkan Balci S, Severe T,
Balat A. Role of cytokine gene (IFN-γ, TNF-α, TGF-β1, IL-6,
and IL-10) polymorphisms in pathogenesis of acute rheumatic
fever in Turkish children. Eur J Pediatr 2012;171:1103–8.
- 15. Rossi C. Bruno de Finetti: the mathematician, the statistician,
the economist, the forerunner. Stat Med 2001;20:3651-66.
- 16. Ben Selma W, Harizi H, Bougmiza I, et al. Interferon gamma
+874 T/A polymorphism is associated with susceptibility to
active pulmonary tuberculosis development in Tunisian patients.
DNA Cell Biol 2011;30:379–87.
- 17. Chong WP, Ip WK, Tso GH, et al. The interferon gamma gene
polymorphism +874 T/A is associated with severe acute respiratory
syndrome. BMC Infect Dis 2006;4:82–9.
- 18. Tangwattanachuleeporn M, Sodsai P, Avihingsanon Y, et al.
Association of interferon gamma gene polymorphism (+874
T/A) with arthritis manifestation in SLE. Clin Rheumatol 2007:
26;1921–4.
- 19. Whiteman SC, Spiteri MA. IFN-gamma regulation of ICAM-I
receptors in bronchial epithelial cells: soluble ICAM-I release
inhibits human rhinovirus infection. J Inflamm 2008;5:5–8.
- 20. Doyle WJ, Casselbrant ML, Li-Korothy HS, et al. The IL-6 (-
174, C/C) genotype predicts greater rhinovirus illness. J Infect
Dis 2010;101:199–206.
- 21. Matkovic S, Vojvodic D, Baljosevic I. Comparison of cytokine
levels in bilateral ear effusions in patients with otitis media secretoria.
Otolarngol Head Neck Surg 2007;137:450–3.
- 22. Aminpour S, Tinling SP, Brodie HA. Role of tumor necrosis factor-
· in sensorineural hearing loss after bacterial meningitis. Otol
Neurotol 2005;26:602–9.