Yaser Said ÇETİN, Ufuk DÜZENLİ, Mehmet BERKÖZ, Hüseyin ÖZKAN, Nazım BOZAN

An investigation of 8-hydroxy-2’-deoxyguanosine and 8-isoprostaglandin F2α levels in patients with larynx carcinoma

Objective: 8-hydroxy-2’-deoxyguanosine (8-OHdG) and 8-iso-prostaglandin F2α (8-iso-PGF2α) are indicators of oxidative stress in the human body. The aim of the present study was to investigate serum 8-OHdG and 8-iso-PGF2α levels in patients with larynx carcinoma and to compare them with healthy controls. Methods: A total of 50 patients, consisting of 25 patients with larynx carcinoma (study group) and 25 healthy subjects (control group), were enrolled in the present study. Serum 8-OHdG concentration was measured using an ELISA kit, whereas an enzyme immunoassay kit was utilized for the measurement of serum 8-iso-PGF2α levels. TNM stages of the patients were recorded from the patient records in the study group. Results: 8-OHdG and 8-iso-PGF2α levels were significantly higher in patients with larynx carcinoma than the control subjects. 8-OHdG levels were significantly higher in patients with LN metastases than the patients without LN metastases in the study group. Conclusion: The findings of the present study showed the presence of significantly increased serum 8-OHdG and 8-iso-PGF2α levels in patients with larynx carcinoma, which may support the role of oxidative stress in the development laryngeal cancers.

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1. Zhang SS, Xia QM, Zheng RS, Chen WQ. Laryngeal cancer incidence and mortality in China, 2010. J Cancer Res Ther 2015;11:143-8.

2. Steuer CE, El-Deiry M, Parks JR, Higgins KA, Saba NF. An update on larynx cancer. CA Cancer J Clin 2017;67:31-50.

3. Sies H. Oxidative stress: a concept in redox biology and medicine. Redox Biol 2015;4:180-3.

4. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 2010;4:118- 26.

5. Sies H, Stahl W, Sundquist AR. Antioxidant functions of vitamins. Vitamins E and C, beta-carotene, and other carotenoids. Ann N Y Acad Sci 1992;669:7-20.

6. Siddartha SB, Kumari S, Rathan S, Babu S, Shetty S. Status of serum sialic acid, antioxidant and lipid peroxidation in head and neck cancer patients. Res J Pharma Biol Chem Sci 2011;2:1-5.

7. Nagai MA. Genetic alterations in head and neck squamous cell carcinomas. Braz J Med Biol Res 1999;32:897-904.

8. Mazlumoglu MR, Ozkan O, Alp HH, et al. Measuring Oxidative DNA Damage With 8-Hydroxy-2’-Deoxyguanosine Levels in Patients With Laryngeal Cancer. Ann Otol Rhinol Laryngol 2017;126:103-9.

9. He H, Zhao Y, Wang N, Zhang L, Wang C. 8-Hydroxy-2’-deoxyguanosine expression predicts outcome of esophageal cancer. Ann Diagn Pathol 2014;18:326-8.

10. Kumar A, Pant MC, Singh HS, Khandelwal S. Determinants of oxidative stress and DNA damage (8-OhdG) in squamous cell carcinoma of head and neck. Indian J Cancer 2012;49:309-15.

11. Aksoy A, Kurnaz SÇ. An investigation of oxidative stress and coenzyme Q10 levels in patients with head and neck squamous cell carcinomas. Eur Arch Otorhinolaryngol 2019;276:1197-204.

12. Basu S. Metabolism of 8-iso-prostaglandin F2alpha. FEBS Lett 1998;428:32-6.

13. Basu S. Fatty acid oxidation and isoprostanes: oxidative strain and oxidative stress. Prostaglandins Leukot Essent Fatty Acids 2010;82:219-25.

14. Dogan R, Meriç Hafiz A, Tugrul S, Ozturan O, Keskin S, Kocyigit A. Can Oxidative Stress Parameters Be Used as Biomarkers for the Discrimination of Malignant Head and Neck Tumors. J Craniofac Surg 2016;27:316-20.

15. Bozan N, Demir H, Gürsoy T, et al. Alterations in oxidative stress markers in laryngeal carcinoma patients. J Chin Med Assoc 2018;81:811-5.

16. Karaman E, Uzun H, Papila I, et al. Serum paraoxonase activity and oxidative DNA damage in patients with laryngeal squamous cell carcinoma. J Craniofac Surg 2010;21:1745-9.

17. Kaur J, Politis C, Jacobs R. Salivary 8-hydroxy-2-deoxyguanosine, malondialdehyde, vitamin C, and vitamin E in oral pre-cancer and cancer: diagnostic value and free radical mechanism of action. Clin Oral Investig 2016;20:315-9.

18. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 2006;160:1-40.

19. Marczynski B, Rozynek P, Kraus T, Schlösser S, Raithel HJ, Baur X. Levels of 8-hydroxy-2’-deoxyguanosine in DNA of white blood cells from workers highly exposed to asbestos in Germany. Mutat Res 2000;468:195-202.

20. Brys M, Morel A, Forma E, et al. Relationship of urinary isoprostanes to prostate cancer occurrence. Mol Cell Biochem 2013;372:149-53.

21. Gào X, Brenner H, Holleczek B, et al. Urinary 8-isoprostane levels and occurrence of lung, colorectal, prostate, breast and overall cancer: Results from a large, population-based cohort study with 14 years of follow-up. Free Radic Biol Med 2018;123:20-6.

22. Senghore T, Li YF, Sung FC, et al. Biomarkers of Oxidative Stress Associated with the Risk of Potentially Malignant Oral Disorders. Anticancer Res 2018;38:4661-6.

23. Asami S, Manabe H, Miyake J, et al. Cigarette smoking induces an increase in oxidative DNA damage, 8-hydroxydeoxyguanosine, in a central site of the human lung. Carcinogenesis 1997;18:1763-6.

24. Morrow JD, Frei B, Longmire AW, et al. Increase in circulating products of lipid peroxidation (F2-isoprostanes) in smokers. Smoking as a cause of oxidative damage. N Engl J Med 1995;332:1198-203.