A comprehensive study of oxidative stress in patients with chronic otitis media

Objective: The purpose of this research study was to evaluate dynamic thiol/disulphide homeostasis (TDH) as a novel oxidative stress marker in participants with noncholesteatomatous chronic otitis media (ncCOM) and cholesteatomatous chronic otitis media (cCOM), as well as to investigate lipid hydroperoxide (LOOH), total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) markers and compare the results with a healthy control group. Methods: This research was carried out at Harran University Medical Faculty Ear-Nose-Throat Department, between April 2017 and April 2019. This was a prospective controlled trial study including 121 participants, 40 ncCOM, 39 cCOM and 42 healthy controls. Total thiol, native thiol, disulphide levels and LOOH, TAS, TOS levels were measured in plasma of all patients and healthy volunteers. Results: In our study, we found that total thiol, native thiol levels, native thiol/total thiol ratios and TAS levels were significantly lower in cCOM patients compared with the control group (p

PDF

___

1. Goycoolea MV, Hueb MM, Ruah C. Otitis media: the pathogenesis approach. Definitions and terminology. Otolaryngol Clin North Am 1991;24:757-61.

2. Kemppainen HO, Puhakka HJ, Laippala PJ, Sipila MM, Manninen MP, Karma PH. Epidemiology and aetiology of middle ear cholesteatoma. Acta Otolaryngol 1999;119:568-72.

3. Celik M, Koyuncu İ. A Comprehensive Study of Oxidative Stress in Tinnitus Patients. Indian J Otolaryngol Head Neck Surg 2018;70:521-6.

4. Baysal E, Aksoy N, Kara F, et al. Oxidative stress in chronic otitis media. Eur Arch Otorhinolaryngol 2013;270:1203-8.

5. Yariktas M, Doner F, Dogru H, Yasan H, Delibas N. The role of free oxygen radicals on the development of otitis media with effusion. Int J Pediatr Otorhinolaryngol 2004;68:889-94.

6. Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014;47:326-32.

7. Cremers CM, Jakob U. Oxidant sensing by reversible disulfide bond formation. J Biol Chem 2013;288:26489-96.

8. Celik M, Koyuncu İ. Oxidative stress in prelingual sensorineural hearing loss and the effects of cochlear implant application on serum oxidative stress levels. Int J Pediatr Otorhinolaryngol 2019;119:177-82.

9. Girotti AW. Lipid hydroperoxide generation, turnover, and effector action in biological systems. J Lipid Res 1998;39:1529-42.

10. Erel O. Automated measurement of serum ferroxidase activity. Clin Chem 1998;44:2313-9.

11. Nourooz-Zadeh J. Ferrous ion oxidation in presence of xylenol orange for detection of lipid hydroperoxides in plasma. Methods Enzymol 1999;300:58-62.

12. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005;38:1103-11.

13. Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 2004;37:112-9.

14. Gupta RK, Patel AK, Shah N, et al. Oxidative stress and antioxidants in disease and cancer: a review. Asian Pac J Cancer Prev 2014;15:4405-9.

15. Ates I, Kaplan M, Yuksel M, et al. Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine 2016;51:47-51.

16. Yuksel M, Ates I, Kaplan M, et al. The dynamic thiol/disulphide homeostasis in inflammatory bowel disease and its relation with disease activity and pathogenesis. Int J Colorectal Dis 2016;31:1229-31.

17. Şimşek E, Erel O, Bicer CK, Çarlıoğlu A. A novel method for determining the relation between nasal polyposis and oxidative stress: the thiol/ disulphide homeostasis. Acta Otolaryngol 2016;136:1180-3.

18. Cross CE, Halliwell B, Borish ET, et al. Oxygen radicals and human disease. Ann Intern Med 1987;107:526-45.

19. Porter NA, Mills KA, Caldwell SE. Mechanisms of free radical oxidation of unsaturated lipids. Lipids 1995;30:277-90.

20. Semaan MT, Megerian CA. The pathophysiology of cholesteatoma. Otolaryngol Clin North Am 2006;39:1143-59.

21. Karlidag T, Ilhan N, Kaygusuz I, Keles E, Yalcin S. Comparison of free radicals and antioxidant enzymes in chronic otitis media with and without tympanosclerosis. Laryngoscope 2004;114:85-9.

22. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39:44-84.

23. Tos M. Sequelae of secretory otitis media and the relationship to chronic suppurative otitis media. Ann Otol Rhinol Laryngol 1990;99:18-9.

24. Döner F, Delibas N, Dogru H, Yariktas M, Demirci M. The role of free oxygen radicals in experimental otitis media. J Basic Clin Physiol Pharmacol 2002;13:33-40.

25. Yılmaz T, Koçan EG, Besler HT, Yılmaz G, Gürsel B. The role of oxidants and antioxidants in otitis media with effusion in children. Otolaryngol Head Neck Surg 2004;131:797-803.

26. Khakimov AM, Arifov SS, Faizulaeva FN. Efficacy of antioxidant therapy in patients with acute and chronic purulent otitis media. Vestn Otorinolaringol 1997;5:16-9.

27. Bernstein V. Middle ear mucosa histological, histochemical immunochemical and immunologic aspects. In: John AF, Sontos-Sacchi J, eds. Physiology of the ear. New York, NY: Raven Press; 1988. p. 59-80.

28. Hamzei M, Ventriglia G, Hagnia M, et al. Osteoclast stimulating and differentiating factors in human cholesteatoma. Laryngoscope 2003;113:436-42.

29. Jung JY, Pashia ME, Nishimoto SY, Faddis BT, Chole RA. A possible role for nitric oxide in osteoclastogenesis associated with cholesteatoma. Otol Neurotol 2004;25:661-8.

30. Camps J, Pujol I, Ballester F, Joven J, Simo´ JM. Paraoxonases as potential antibiofilm agents: their relationship with quorum-sensing signals in gram-negative bacteria. Antimicrob Agents Chemother 2011;55:1325- 31.