The adoption of sorghum as alternative souce of food in Indonesia depends on consummer acceptance of sorghum grain which is determine by the nutritional and anti-nutirtional values and the quality of sorghum grain. The objectives of this study were to obtain information on genetic variability of sorghum genotypes with diverse backgroud for nutritional values and the effect of genetic x environment on the variablity in sorghum grain nutritional values. The study was conducted on 24 sorghum lines consisted of breeding lines, introduced lines and a local variety. The lines were planted in a randomized complete block design with tree replications in two locations differing in soil fertility. The results showed that the protein, fat, amylose and tannin contents of the sorghum lines are effected by the genotype x environment interaction with different magnitutes. The presence of G X E interaction reduced the genetic variances and estimates of heritability of the characters