Mesut NAR, Hande Nur ALBEZİRGAN1, Burcu D EMİRKAYA, Hakan FİDAN, Hakan AKTAŞ, Ş. Şebnem ELLİALTIOĞLU

Accelerated Pepper Breeding Using Molecular Markers and Doubled Haploidy Technique

Hatay pepper is preferred in terms of quality characteristics e.g. hot, fresh and suitable for dried consumption, but it does not contain any disease resistance genes. Within the scope, we established the study is aiming to develop resistant hybrid varieties to TSWV is causing high yield and quality losses in pepper production areas. For this purpose, studies are carried out to obtain inbreed resistant lines in a short time, a Tsw gene found in Capsicum chinense, which provides resistance to TSWV, was combined with Hatay pepper lines by crossbreeding. First of all, 20 of the Hatay pepper lines from the gene pool were selected according to weighted grading criteria, then the number was reduced to 4. In C. chinense lines, molecular screening was performed with SCAC568 primers and individuals with Tsw gene were determined from donor parents and crosses. After then, backcrosses to Hatay peppers were conducted and BC1F1 was generated. Their seed samples were sown from them and brought until the first true leaves during the transition from seed to seedling stage. Meanwhile, molecular analyses were applied to find resistant individuals with Tsw gene. The homozygous and heterozygous plants were planted in a greenhouse and used in anther culture study. Whole study takes 22 months from the initiation of hybridization to the emergence of androgenic embryos and acclimatization to external conditions and development of DH seeds. Here, these findings are presented as a case study in biotechnology and the combined techniques are an indispensable part of accelerated breeding processes.

Keywords:

Anther culture, Capsicum spp., Tsw gene, Hatay pepper, molecular marker,

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