Fatma Gül GÖZE ÖZDEMİR, Bekir TOSUN, Arif ŞANLI, Tahsin KARADOĞAN

Bazı Apiaceae uçucu yağlarının Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Nematoda: Meloidogynidae)'ya karşı nematoksik etkisi

Amaç: Çalışmada Apiaceae familyasına ait kültürü yapılan ve doğal florada yayılış gösteren bazı türlerin meyve uçucu yağlarının in vitro ve in vivo koşullarda Meloidogyne incognita’ya karşı nematoksik etkilerinin belirlenmesi amaçlanmıştır. Materyal ve Yöntem: in vitro çalışmalarda 125, 250, 500 ve 1000 ppm uçucu yağ konsantrasyonlarının ikinci dönem larva (L2) üzerine etkisi araştırılmıştır. In vivo çalışmalar bitki büyütme kabinlerinde domates bitkisinde uçucu yağların 1000 ppm konsantrasyonları ile yürütülmüştür. Çalışma 9 hafta sonra sonlandırılmış ve köklerde gal ve yumurta paketi sayısı belirlenmiştir. Araştırma Bulguları: En yüksek nematoksik etki in vitro koşullarda % 83.3 ölüm oranı ile Ferulago paucuiradiata uçucu yağının 1000 ppm dozunda belirlenmiştir. Ferulago pauciradiata ve Foeniculum vulgare uçucu yağı uygulanan bitkilerin köklerinde gal ve yumurta paketi sayısı daha düşük olmuştur. Sonuç: Meloidogyne incognita’ya karşı in vitro ve in vivo koşullarda F. pauciradiata ve F. vulgare uçucu yağlarının yüksek nematoksik etki gösterdiği saptanmıştır.

Anahtar Kelimeler:

Apiaceae, Biyolojik Mücadele, Kök ur Nematodu, Uçucu Yağ

Nematoxic activity of some Apiaceae essential oils against Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Nematoda: Meloidogynidae)

Objective: The objective of this study was to determine in vitro and in vivo nematoxic effects of cultivated and naturally distributed fruit essential oils (EO’s) of Apiaceae species against Meloidogyne incognita. Material and Methods: The effects of 125, 250, 500 and 1000 ppm EO’s concentrations on the second juvenile larvae (J2) were investigated in vitro. In vivo studies were carried out in climate room using tomato seedlings at 1000 ppm concentrations of EO’s.The number of galls and egg masses in the roots was determined after 9 weeks. Results: A 1000 ppm concentration of Ferulago paucuiradiata EO showed the highest nematotoxic effect with a mortality rate of 83.3% in vitro. The number of gall and egg masses in the roots treated with F. pauciradiata and Foeniculum vulgare EO’s was lower. Conclusion: Ferulago pauciradiata and F. vulgare EO’s have strong nematoxic effects against M. incognita in vitro and in vivo.

Keywords:

Apiaceae, Biological control, Root knot nematode, Essential oil,

PDF

___

Adam, M. A. M., M. S. Phillips & V. C. Blok, 2007. Molecular diagnostic key for identification of single juveniles of seven common and economically important species of root-knot nematode (Meloidogyne spp.). Plant Pathology, 56: 190-197. Doi: 10.1111/j.1365-3059.2006.01455.x
Aslan, A., & İ. H. Elekcioğlu, 2022. Biochemical and molecular identification of root-knot nematodes in greenhouse vegetable areas of Eastern Mediterranean Region (Turkey). Turkish Journal of Entomology, 46 (1): 115-127.
Bai, P.H., C.Q. Bai, Q.Z. Liu, S.S. Du & Z.L. Liu, 2013. Nematicidal activity of the essential oil of Rhododendron anthopogonoides aerial parts and its constituent compounds against Meloidogyne incognita. Zeitschrift für Naturforschung, 68 (c): 307-312.
Bartlem, D. G., M.G. Jones & U.Z. Hammes, 2014. Vascularization and nutrient delivery at root-knot nematode feeding sites in host roots. Journal of Experimental Botany, 65 (7): 1789-1798. https://doi.org/10.1093/jxb/ert415
Brodie, B.B., K. Evans & J. Franco, 1993. “Nematode Parasites of Potato, 87-132”. In: Plant Parasitic Nematodes in Temperate Agriculture (Eds. K. Evans, D. L. Trudgill & J.M. Webster). CAB International, Wallingford, UK, 656 pp.
Cavanagh, H. & M.A. Cavanagh, 2007. Antifungal activity of the volatile phase of essential oils: A brief review, Natural Product Communications, 2: 1297-1302.
Cayrol, J.C., C. Djian & L. Pijarowski, 1989. Study of the nematicidal properties of the culture filtrate of the nematophagous fungus Paecilomyces lilacinus. Revue de Nematologie, 12 (4): 331-336.
Chitwood, D.J., 2002. Phytochemical based strategies for nematode control. Annual Review Of Phytopathology, 40 (1): 221-249.
D’Addabbo, T., T. Carbonara, M.P. Argentieri, V. Radicci, P. Leonetti, L. Villanova & P. Avato, 2013. Nematicidal potential of Artemisia annua and its main metabolites. European Journal of Plant Pathology, 137 (2): 295-304.
Devran, Z. & M.A. Söğüt, 2009. Distribution and identification of root-knot nematodes from Turkey. Journal of Nematology, 41 (2): 128-133.
Dias, C.R., S.L. Maciel, J.B. Vida & C.A. Scapim, 1988. Efeito de quatro espécies de plantas medicinais sobre Meloidogyne incognita (Kofoid & White, 1919) Chitwood,1949 em protegido. Nematologia Brasileira, 22 (2): 58-65.
Dorman, H.J.D. & S.G. Deans, 2000. Antimicrobial agents from plants: Antibacterial activity of plant volatile oils. Journal Applied Microbiology, 88 (2): 308-316.
Echeverrigaray, S., J. Zacaria & R. Beltrão, 2010. Nematicidal activity of monoterpenoids against the root-knot nematode Meloidogyne incognita. Phytopathology,100 (2): 199-203.
Eder, R., E. Consoli, J. Krauss & P. Dahlin, 2021. Polysulfides Applied as Formulated Garlic Extract to Protect Tomato Plants against the Root-Knot Nematode Meloidogyne incognita.Plants,10 (2): 394-494.
El-Habashy, D.E., M.A.A. Rasoul & S.A. Abdelgaleil, 2020. Nematicidal activity of phytochemicals and their potential use for the control of Meloidogyne javanica infected eggplant in the greenhouse. European Journal of Plant Pathology,158 (2): 381-390. https://doi.org/10.1007/s10658-020-02079-6
Evlice, E. & Ş .Bayram, 2016. Identification of root-knot nematode species (Meloidogyne spp.) (Nemata: Meloidogynidae) in the potato fields of Central Anatolia (Turkey) using molecular and morphological methods. Türkiye Entomoloji Bülteni, 6 (4):339-347.
Faria, J. M. S., I. Sena, B. Ribeiro, A. M. Rodrigues, C. M. N. Maleita, I. Abrantes & A. C.da Silva Figueiredo, 2016. First report on Meloidogyne chitwoodi hatching inhibition activity of essential oils and essential oils fractions. Journal of Pest Science, 89 (1): 207-217. https://doi.org/10.1007/s10340-015-0664-0
Geç, S., F.G. Göze Özdemir & B. Yaşar, 2018. “İn vitro koşullarda bazı bitkisel yağların Meloidogyne incognita (Kofoid & White, 1919) ırk 2'ye karşı nematisidal etkilerinin belirlenmesi, 78-87”. Proceeding Book of 6th International Congress of Agriculture and Environment,11-13 October, Antalya, Turkey, 469 pp.
Ghezelbash, N. & M. Abdolahi, 2013. In vitro inhibition of Root-knot nematode, Meloıdogyne javanica by aqueous extract of Zatarıa multıflora and Ferulago angulata and some of their compounds. Journal of Research in Plant Pathology, 2 (1): 51-60.
Göze Özdemir, F. G., B. Tosun, A. Şanlı & T. Karadoğan, 2021.Türkiye’de yetişen bazı Apiaceae türlerinin uçucu yağlarının Kök lezyon nematodlarına karşı nematisidal aktiviteleri. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 31 (2): 425-433. https://doi.org/10.29133/yyutbd.796093
Göze Özdemir, F. G., Ş. E. Arıcı & İ. H. Elekcioğlu, 2022. Interaction of Meloidogyne incognita (Kofoid & White, 1919) (Nemata:Meloidogynidae) and Fusarium oxysporum f. sp. radicis-lycopersici Jarvis & Shoemaker in tomato F1 hybrids with differing levels of resistance to these pathogens. Turkish Journal of Entomology, 46 (1): 63-73.
Göze Özdemir, F.G. & G. Uysal, 2018. Meloidogyne incognita ırk 2’nin farklı inokulasyon yoğunluklarının bazı dayanıklı biber hatlarında reaksiyonu. Ege Üniversitesi Ziraat Fakültesi Dergisi, 55 (2): 161-170. https://doi.org/10.20289/zfdergi.408828
Gupta A., S. Sharma & S.N. Naik, 2011. Biopesticidal value of selected EOs against pathogenic fungus, termites, and nematodes. International Biodeterioration Biodegradation, 65 (5): 703-707.
Gürkan, B., R. Çetintaş & T. Gürkan, 2019. Gaziantep ve Osmaniye Sebze Alanlarında Bulunan Kök-ur Nematodu Türleri (Meloidogyne spp.)’nin Teşhisi ile Bazı Nematod Popülasyon Irklarının Belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 22 (Ek sayı 1): 113-124. (in Turkish with abstract in English).
Hallmann, J. & S. Kiewnick, 2018. Virulence of Meloidogyne incognita populations and Meloidogyne enterolobii on resistant cucurbitaceous and solanaceous plant genotypes.Journal of Plant Diseases and Protection, 125 (4): 415-424.
Hemaiswarya, S., A.K. Kruthiventi & M. Doble, 2008. Synergism between natural products and antibiotics against infectious diseases. Phytomedicine, 15 (8):639-652.
Hoet, S., C. Ste´vigny, M.F. He´rent & J. Quetin-Leclercq, 2006. Antitry-panosomal compounds from leaf essential oil of Strychnos spinosa. Planta Medica,72 (5): 480-482.
Ipek, E., H. Zeytinoglu, S. Okay, B.A.Tuylu, M. Kurkcuoglu & K.H.C Baser, 2005. Genotoxicity and antigenotoxicity of Origanum oil and carvacrol evaluated by Ames Salmonella/microsomal test. Food Chemistry, 93 (3): 551-556.
Javed, N., S.R. Gowen, M. Inam-ul-Haq & S.A. Anwar, 2007. Protective and curative effect of neem (Azadirachta indica) formulations on the development of root-knot nematode Meloidogyne javanica in roots of tomato plants. Crop Protection, 26 (4): 530-534.
Kabera, J.N., E. Semana, A.R. Mussa & X. He, 2014. Plant secondary metabolites: Biosynthesis, classification, function and pharmacological properties. Journal of Pharmacy and Pharmacology, 2 (7): 377-392.
Kepenekci, I., D. Erdoğuş & P. Erdoğan, 2016. Effects of some plant extracts on root-knot nematodes in vitro and in vivo conditions. Turkish Journal of Entomology, 40 (1): 3-4.
Kepenekci, İ. & O. Dura, 2017. Anethum graveolens, a new host of Root-knot nematode (Meloidogyne incognita) in Turkey. Pakistan Journal of Nematology, 35 (2): 215-216. http://dx.doi.org/10.18681/pjn.v35.i02.p215-216
Kong J., Lee S., Moon Y., Lee S., & Ahn Y. (2006). Nematicidal activity of plant EOs against Bursaphelenchus xylophilus (Nematoda: aphelenchoididae). Journal of Asia Pacific Entomology, 9 (2): 173-178.
Lang, G. & G. Buchbauer, 2012. A review on recent research results (2008-2010) on essential oils as antimicrobials and antifungals. Flavour Fragrance Journal, 27 (1): 13-39.
Langeveld, W.T., E.J.A. Veldhuizen & S.A. Burt, 2014. Synergy between essential oil components and antibiotics: a review. Critical Reviews in Microbiology, 40 (1): 76-94, DOI: 10.3109/1040841X.2013.763219
Marotti, M. & R. Piccaglia, 1992. The influence of distillation conditions on the essential oil composition of three Varieties of Foeniculum vulgare Mill. Journal of Essential Oil Research, 4 (6): 569-576.
Menjivar, R.D., A.A. Dababat & R.A. Sikora, 2012. Biological control of Meloidogyne incognita on cucurbitaceous crops by the non-pathogenic endophytic fungus Fusarium oxysporum strain162. International Jrournal of Pest Management 57 (3): 70-72.
Miladi, H., T. Zmantar, B. Kouidhi, Y.M.A. Al Qurashi, A. Bakhrouf & Y. Chaabouni, 2017. Synergistic effect of eugenol, carvacrol, thymol, p-cymene and γ-terpinene on inhibition of drug resistance and biofilm formation of oral bacteria. Microbial Pathogenesis, 112: 156-63.
Ntalli, N.G., F. Ferrari, I. Giannakou & U. Menkissoglu-Spiroudi, 2010. Phytochemistry and nematicidal activity of the essential oils from 8 Greek Lamiaceae aromatic plants and 13 terpene components, Journal of Agriculture Food Chemistry, 58 (13): 7856-7863.
Ntalli, N.G., F. Ferrari, I. Giannakou & U. Menkissoglu-Spiroudi, 2011. Synergistic and antagonistic interactions of terpenes against Meloidogyne incognita and the nematicidal activity of EOs from seven plants indigenous to Greece. Pest Management Science, 67 (3): 341-351.
Oka, Y., S. Nacar, E. Putievsky, U. Ravid, Z. Yaniv & Y. Spiegel, 2000. Nematicidal activity of essential oils and their components against the root-knot nematode. Phytopathology, 90 (7): 710-715.
Ozdemir, E. & U. Gozel, 2017. Efficiency of some plant essential oils on root-knot nematode Meloidogyne incognita. Journal of Agricultural Science and Technology A, 7 (3): 178-183.
Özarslandan, A. & İ. H. Elekcioğlu, 2010. Identification of the Root-knot nematode species (Meloidogyne spp.) (Nemata: Meloidogynidae) collected from different parts of Turkey by moleculer and morphological methods.Turkish Journal of Entomology, 34 (3): 323-335.
Pardavella, I., D. Daferera, T. Tselios, P. Skiada & I. Giannakou, 2021. The use of essential oil and hydrosol extracted from Cuminum cyminum seeds for the control of Meloidogyne incognita and Meloidogyne javanica. Plants, 10 (1): 46-60. https://doi.org/10.3390/plants10010046
Saad, A.S.A., M.B., Al-Kadi, A.A.A. Deeabes & A.M. El-Kholy, 2018. Nematicidal performance of certain organic and inorganic compounds against Meloidogyne incognita infecting okra plants. Pakistan Journal of Nematology,36 (2): 177-189. http://dx.doi.org/10.18681/pjn.v36.i02.p177-189
Santana-Rios, G., G.A. Orner, A. Amantana, C.Provost, S.Y. Wu & R.H. Dashwood, 2001. Potent antimutagenic activity of white tea in comparison with green tea in the Salmonella assay. Mutation Research, 495 (1-2): 61-74.
Sellami, S., L. Reguieg & T. Dahmane, 2013. Effectiveness of essential oils of Mentha spicata (Lamiaceae) and Foeniculum vulgare (Apiaceae) against Meloidogyne incognita (Nematoda: Meloidogynidae). Bulletin de la Société zoologique de France, 138 (1/4): 139-149.
Siddiqui, A. & M.J. Zaki, 2017. Efficacy of some seeds of family apiaceae against root knot Nematode, Meloidogyne javanica (Treub) Chitwood. International Journal of Biology and Biotechonology, 14 (1): 89-94.
Stavropoulou, E., E. Nasiou, P. Skiada & I.O. Giannakou, 2021. Effects of four terpenes on the mortality of Ditylenchus dipsaci (Kühn) Filipjev. European Journal of Plant Pathology, 160 (1): 137-146. https://doi.org/10.1007/s10658-021-02229-4
Stein S.E., 1990. National Institute of Standardsand Technology (NIST) Mass Spectral Database and Software, Version 3.02, Juen USA.
Tapia-Vázquez, I., A. C. Montoya-Martínez, D. los Santos-Villalobos, M. J. Ek-Ramos, R. Montesinos-Matías & C. Martínez-Anaya, 2022. Root-knot nematodes (Meloidogyne spp.) a threat to agriculture in Mexico: biology, current control strategies, and perspectives. World Journal of Microbiology and Biotechnology, 38 (2): 1-18.
Tsao, R. & Yu Q. 2000. Nematicidal activity of monoterpenoid compounds against economically important nematodes in agriculture. Journal of Essential Oil Research, 12 (3):350-354.
Turatto, M.F., F.D.S. Dourado, J.E. Zilli & G.R. Botelho, 2018. Control potential of Meloidogyne javanica and Ditylenchus spp. using fluorescent Pseudomonas and Bacillus spp. Brazilian Journal of Microbiology, 49: 54-59. doi: 10.1016/j.bjm.2017.03.015
Udo, I.A., M.I. Uguru, R.O. Ogbuji, & D.A. Ukeh, 2008. Sources of tolerance to root-knot nematode, Meloidogyne javanica, in cultivated and wild tomato species. Plant Pathology Journal, 7 (1): 324-329.
Ultee, A., M.H.J. Bennik & R. Moezelaar, 2002. The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Applied Environment Microbiology, 68 (4): 1561-1568.
Uysal, G., M.A. Söğüt & İ.H Elekçioğlu, 2017. Identification and distribution of root-knot nematode species (Meloidogyne spp.) in vegetable growing areas of Lakes Region in Turkey. Turkish Journal of Entomology, 41 (1): 105-122. https://doi.org/10.16970/ted.91225
Wang, K.H., R. McSorley & N. Kokalis-Burelle, 2006. Effects of cover cropping, solarization, and soil fumigation on nematode communities. Plant and Soil, 286 (1): 229-243.
Youssef, M., H. Abd Abd-El-Khair & W.M. El-Nagdi, 2017. Management of root knot nematode, Meloidogyne incognita infecting sugar beet as affected by certain bacterial and fungal suspensions. Agricultural Engineering International: CIGR Journal, Special issue: 293-301.
Zhou, L., J. Wang, K. Wang, J. Xu, J. Zhao, T. Shan & C. Luo, 2012. Secondary metabolites with antinematodal activity from higher plants. In Studies in Natural Products Chemistry, 37: 67-114.