Effect of intermittent fasting during acrylamide exposure on blood copper, zinc and lead levels in rats

Zinc and copper are responsible for certain physiological functions in the body. On the other hand lead is a toxic trace element. In this study, effect of intermittent fasting on blood zinc, copper and lead levels during acrylamide exposure was studied. Forty female adult Wistar albino rats were divided into 4 groups (n=10 in each group). First group: control group without any administration, second group: acrylamide group in which 20 mg/kg/day acrylamide was administered for 30 days, third group: intermittent fasting group in which 12 hours of fasting were administered overnight for 30 days, fourth group: intermittent fasting and acrylamide group in which 20 mg/kg/day acrylamide and 12 hours of intermittent fasting was administered for 30 days. At the last day of experimental procedure blood was withdrawn under anesthesia. Zinc as well as copper levels were measured from plasma samples in flame unit and lead was measured from erythrocyte packages in combustion unit of Atomic Absorption Spectrophotometer. In acrylamide group, zinc was lower (p0.05) and lead levels (p

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Lineback DR, Coughlin JR, Stadler RH. Acrylamide in foods: a review of the science and future considerations. Annu Rev Food Sci Technol 2012; 3: 15-35.

Mottram DS, Wedzicha BL, Dodson AT. Acrylamide is formed in the Maillard reaction. Nature 2002; 419: 448-449.

Parzefall W. Minireview on the toxicity of dietary acrylamide. Food Chem Toxicol 2008; 46: 1360-1364.

Bentzen BH, Schmitt N, Calloe K, et al. The acrylamide (S)-1 differentially affects Kv7 (KCNQ) potassium channels. Neuropharmacology 2006; 51: 1068-1077.

Sumner SC, Willams CC, Snyder RW, et al. Acrylamide: A Comparison of Metabolism and Hemoglobin Adducts in Rodents following Dermal, Intraperitoneal, Oral, or Inhalation Exposure. Toxicol Sci 2003; 75: 260-270.

Arihan O, Seringeç NB, Gürel Eİ, Dikmenoğlu NH. Effects of oral acrylamide intake on blood viscosity parameters in rats. Clin Hemorheol Microcirc 2011; 47: 45-52.

Ghorbel I, Maktouf S, Kallel C, et al. Disruption of erythrocyte antioxidant defense system, hematological parameters, induction of pro-inflammatory cytokines and DNA damage in liver of co-exposed rats to aluminium and acrylamide. Chemico-Biol Interact 2015; 236: 31-40.

Sohal RS, Weindruch R. Oxidative stress, caloric restriction, and aging. Science 1996; 273: 59-63.

Mattson MP, Chan SL, Duan W. Modification of brain aging and neurodegenerative disorders by genes, diet, and behavior. Physiol Rev 2002; 82: 637-672.

Tikoo K, Tripathi DN, Kabra DG, Sharma V, Gaikwad AB. Intermittent fasting prevents the progression of type I diabetic nephropathy in rats and changes the expression of Sir2 and p53. FEBS Letters 2007; 581: 1071-1078.

Stefanidou M, Maravelias C, Dona A, Spiliopoulou C. Zinc: a multipurpose trace element. Arch Toxicol 2006; 80: 1-9.

Maret W, Sandstead HH. Zinc requirements and the risks and benefits of zinc supplementation. J Trace Elem Med Bio 2006; 20: 3-18.

Salgueiro MJ, Zubillaga M, Lysionek A, et al. Zinc as an essential mıcro nutrient: a review. Nutr Res 2000; 20: 737-755.

Guler I, Himmetoglu O, Turp A, et al. Biol Trace Elem Res 2014; 158: 297-304.

Yang HJ, Lee SH, Jin Y, et al. Genotoxicity and toxicological effects of acrylamide on reproductive system in male rats. J Vet Sci 2005; 6: 103-109.

Zhang Y, Wang Q, Zhang G, et al. Biomarker analysis of hemoglobin adducts of acrylamide and glycidamide enantiomers for mid-term internal exposure assessment by isotope dilution ultra-high performance liquid chromatography tandem mass spectrometry. Talanta 2018; 178: 825-833.

Ghorbel I, Maktouf S, Kallel C, et al. Disruption of erythrocyte antioxidant defense system, hematological parameters, induction of pro-inflammatory cytokines and DNA damage in liver of co-exposed rats to aluminium and acrylamide. Chemico-Biol Int 2015; 236: 31-40.

Yahaya MI, Ogunfowokan AO, Orji EO. Elemental profile in amniotic fluid of some Nigerian pregnant women. East Afr J Public Health 2011; 8: 92-97.

Reyes H, Baez ME, Manuel CG, et al. Selenium, zinc and copper plasma levels in intrahepatic cholestasis of pregnancy, in normal pregnancies and in healthy individuals, in Chile. J Hepatol 2000; 32: 542-549.

Huyut Z, Şekeroğlu MR, Balahoroğlu R, Huyut MT. Characteristics of resveratrol and serotonin on antioxidant capacity and susceptibility to oxidation of red blood cells in stored human blood in a time-dependent manner. J Int Med Res 2018; 46: 272-283.

Chan S, Gerson B, Subramaniam S. The role of copper, molybdenum, selenium, and zinc in nutrition and health. Clin Lab Med 1998; 18: 673-685.

Yerlikaya FH, Yener Y. The Dietary Acrylamide Intake Adversely Affects the Serum Trace Element Status. Biol Trace Elem Res 2013; 152: 75-81.

Barany E, Bergdahl IA, Bratteby LE, et al. Relationships between trace element concentrations in human blood and serum. Toxicol Lett 2002; 134: 177-184.

Kamenov G and Gulson BL. The Pb isotopic record of historical to modern human lead exposure. Sci Total Environ 2014; 490: 861- 870.

Talsma EF, Moretti D, Ly SC, et al. Zinc Absorption from Milk Is Affected by Dilution but Not by Thermal Processing, and Milk Enhances Absorption of Zinc from HighPhytate Rice in Young Dutch Women. J Nutr 2017; 147: 1086-1093.

Michielan A, DInca R. Intestinal Permeability in Inflammatory Bowel Disease: Pathogenesis, Clinical Evaluation, and Therapy of Leaky Gut. Mediat Inflamm 2015; 5: 1-10.

Xie XC, Cao YQ, Gao Q, et al. Acupuncture Improves Intestinal Absorption of Iron in Iron-deficient Obese Patients: A Randomized Controlled Preliminary Trial. Chin Med J (Engl) 2017; 130: 508-515.

Heacox HN, Gillman PL, Zwart SR, Smith SM. Excretion of Zinc and Copper Increases in Men during 3 Weeks of Bed Rest, with or without Artificial Gravity. J Nutr 2017; 147: 1113-1120.

Goyer RA. Toxic and Essential Metal Interactions. Ann Rev Nutr 1997; 17: 37-50. 30. Plum LM, Rink L, Haase H. The Essential Toxin: Impact of Zinc on Human Health. Int J Environ Res Public Health 2010; 7: 1342- 1365.