Yüzey Yanıt Metodolojisi İle Biberiye Yaparaklarından Rosmarinik Asit Ekstraksiyonunun Optimizasyonu

Rosmarinik asit, karnozol ve karnosik asit, biberiye (Rosmarinus officinalis) 'de bulunan önemli doğal antioksidandır. Rosmarinik asit (RA), Bu bileşiklerin, özellikle de Rosmarinik asitin biyolojik aktivitelerini rapor eden çok sayıda çalışma bulunmaktadır. Rosmarinik asitin in-vitro çalışmalarda anti-HIV-1, antibakteriyel, antioksidan, anti-kanserojen ve anti-alerjik aktiviteler, antiviral özellikler gibi bazı biyolojik faaliyetleri olduğu bildirilmiştir. In vivo çalışmalar da Rosmarinik Asitin antialerjik, antitromboz ve antikanserojenik özellikler sergilediğini göstermiştir. Endüstri pek çok faydalı etkileri nedeniyle doğal gıda koruyucuları, antimikrobiyal ve antioksidanlar ile giderek daha fazla ilgilenmektedir. Bu çalışmada, biyolojik olarak güçlü bir antioksidan olan rosmarinik asit bileşimi, Biberiye yapraklarından ekstrakte edildi. Ultrasonik Destekli Ekstraksiyon ve Yüzey Yanıt Metodolojisi Biberiye yapraklarından antioksidanların ekstraksiyon koşullarını optimize etmek için kullanılmıştır. En yüksek Rosmarinik Asit düzeyi (% 6,77) için öngörülen optimum koşullar % 34,37 etanol, 37,15 dk ekstraksiyon zamanı ve 73,38 °C ekstraksiyon sıcaklığı olarak tespit edilmiştir. Tahmin edilen optimum koşullarda, deneysel değerler tahmin edilen değerlere çok yakındı.

Anahtar Kelimeler:

Biberiye, Rosmarinik Asit, Ekstraksiyon, Optimizasyon, Yüzey Yanıt Metodolojisi

Optimization of Rosmarinic Acid Extraction from Rosemary Leaves by Surface Response Methodology

Rosmarinic acid (RA), carnosol and carnosic acid are important natural antioxidants found in rosemary (Rosmarinus officinalis). There are number of papers that report the biological activities of this compound, especially RA. RA has been reported to have some biological activities in vitro such as antiviral properties including anti-HIV-1, antibacterial, antioxidant, anti-carcinogenic, and anti-allergic activities. In vivo studies have shown that RA exhibit anti-allergic, anti-thrombotic, and anti-carcinogenic properties as well. Industry is increasingly interested in natural food preservatives, antimicrobial, and antioxidants due to their beneficial effects. In this work, bioactive a potent antioxidant-rosmarinic acid (RA) compound was extracted from Rosmarinus officinalis leaves. Response surface methodology (RSM) has been used to optimize the extraction conditions of antioxidants from Rosemary leaf by using ultrasonic extraction. The predicted optimal conditions for the highest RA level(6.77 %) were found at 34.37 % ethanol, 37.15 min extraction time, and 73.38°C extraction temperature. In the predicted optimal conditions, the experimental values were very close to the predicted values.

Keywords:

Rosmarinus officinalis, Rosmarinic Acid, Extraction, Optimization, Responce Surface Methodology,

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