Saadet DERMİŞ, Sinan BEYAZTAŞ, Zehra Ceren ERTEKİN, Erdal DİNÇ

Spektrofotometrik Yöntemlerle Tabletlerde Metimazol Tayini

Bu çalışma doğrudan absorbans ölçüm ve türev spektrofotometrik yöntemlerini kullanarak tabletlerdeki methimazolün spektrofotometrik miktar tayinini açıklamaktadır. Yapılan ön çalışmalarda sıfırıncı derece ve birinci türev spektrofotometri yöntemlerinde doğrusal çalışma aralığı 2,0-24,0 µg/mL olarak bulunmuştur. Sıfırıncı derece spektrofometri yönteminde 260,0 nm, birinci derece türev spektrofotometri yönteminde ise 269,0 nm dalga boylarında ölçümler yapılarak kalibrasyon eğrileri elde edilmiştir. Geri kazanım ve bağıl standart sapma değerleri; doğrudan absorbans ölçüm yöntemi için % 103,3 ve % 2,18 ve birinci derece türev spektrofotometri için % 98,0 ve % 0,64 olarak bulunmuştur. Daha sonra önerilen teknikler tablet preparatlarının analizi için uygulanmıştır. Bu çalışmada metodların uygulanabilirliği, methimazol içeren test örneklerinin validasyon çalışmalarıyla doğrulanmıştır. Analiz sonuçları, ilacın miktar tayini için ilgili metodların uygulanabilir olduğunu göstermektedir.

Anahtar Kelimeler:

Methimazol, UV-GB Spektrofotometri, Tablet

Determination of Methimazole in Tablets Using Spectrophotometric Methods

This study describes the spectrophotometric quantification of methimazole in tablets using zero-order absorption spectrophotometry and first derivative spectrophotometry methods. In preliminary experiments, linearity range was found between 2.0-24.0 µg/mL in zero-order spectrophotometry and first-order derivative spectrophotometry. Calibration curves were obtained by measurements of the zero-order absorbances and their first derivative values at 260.0 and 269.0 nm for the zero-order spectrophotometry and first derivative spectrophotometry, respectively. The recovery and relative standard deviations were calculated as 103.3 % and 2.18 % for UV absorption spectrophotometry and 98.0% and 0.64% for first derivative spectrophotometry, respectively. The proposed techniques were used for the analysis of tablets preparations. The methods proposed in this study were verified by analyzing validation test samples containing methimazole. Determination results showed that the applied methods were found to suitable for the quantitation of the related drug.

Keywords:

Methimazole, UV-VIS Spectrophotometry, Tablet,

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