İnsan Serum Albümin Nanopartikül/surfaktant Polimer Temelli Sensör ile Metil Paratiyonun Enzimsiz Elektroanalizi

Bu çalışmada, insan serum albümin nanopartiküller (HSA NP) ve elektrobiriktirilmiş yüzey aktif polisetiltrimetil amonyum bromür p(CTAB) film kaplı grafit elektrotlar (PGE) tehlikeli bir çevresel kirletici olan metil paratiyonun (MP) hassas ve seçici tespiti için elektrokimyasal sensör olarak kullanıldı. Elde edilen HSA NP/p(CTAB)/PGE mikroskopik ve elektrokimyasal olarak karakterize edildi. Diferansiyel puls voltametri (DPV) tekniği ile MP için elde edilen doğrusal çalışma aralığı 0,01-0,1 µM ve gözlenebilme sınırı ise 6 nM olarak tespit edildi. MP tespiti için geliştirilen enzim kullanımı gerektirmeyen ve basit hazırlama stratejisine sahip HSA NP/p(CTAB) tabanlı elektrokimyasal sensör, çevre koruma ve gıda güvenliği açısından izleme sürecini de basitleştirme potansiyeline sahiptir.

Anahtar Kelimeler:

Organofosforlu pesitisit, Albumin nanopartikül, Surfaktant polimer, Elektroanaliz, Sensör

Human Serum Albumin Nanoparticles/surfactant Polymer Based Sensor for the Non-enzymatic Electroanalysis of Methyl Parathion

In this study, human serum albumin nanoparticles (HSA NP) and electrodeposited surfactant polycetyltrimethyl ammonium bromide p(CTAB) film coated graphite electrodes (PGE) were utilized as an electrochemical sensor for the sensitive and selective detection of a hazardous environmental pollutant, methyl parathion (MP). The fabricated HSA NP/p(CTAB)/PGE was characterized microscopically and electrochemically. The linear working range obtained for the MP was determined as 0.01-0.1 µM and the detection limit as 6 nM by differential pulse voltammetry (DPV) technique. The HSA NP/p(CTAB) based electrochemical sensor developed for MP detection with a simple preparation strategy and did not require the use of enzymes and has the potential to be applied in environmental protection and food safety monitoring processes.

Keywords:

Organophosphorus pesticide, Albumin nanoparticles, Surfactant polymer, Electroanalysis, Sensor.,

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