Vajinit tanisinda geleneksel yöntemlerle nükleik asit hibridizasyon yöntemlerinin karsilastirilmasi
Çalismanin amaci Candida suslari, Gardnerella vaginalis ve Trichomonas vaginalis tanisinda nükleik asit hibridizasyon teknigi ile mikroskobik inceleme ve kültür gibi geleneksel yöntemlerin karsilastirilmasidir. Kadin Hastaliklari ve Dogum Poliklinigine basvuran ve vajinit tanisi alan 24 hasta çalismaya dahil edildi. Pelvik muayene esnasinda posterior vaginal forniksten alinan örnekler direkt mikroskobik inceleme, Gram boyama, kültür ve hibridizasyon testi için kullanildi. Hibridizasyon testi Gardnerella vaginalis için % 85.7 duyarli ve % 100 özgül bulundu. Testin Candida türleri ve Trichomonas vaginalis için duyarlilik ve özgüllügü % 100 olarak saptandi. Vajinitli olgularin çogunda jinekologlar tedaviyi hemen düzenlemek is tedikleri için nükleik asit hibridizasyon yöntemleri gibi hizli teknikler, kültür ve gram boyama gibi geleneksel yöntemlere ek olarak kullanilabilir. Bu tani yaklasimi tedavi basarisini da artiracaktir
Anahtar Kelimeler:
nükleik asit hibridizasyon testi, Gardnerella vaginalis, Trichomonas vaginalis, kültür
Rapid diagnosis of vaginitis: Conventional Methods Versus Nucleic Acid Hybridization Techniques
The aim of the present study was to compare the results of nucleic acid hybridization technique with microscopic evaluation and culturing in determination of Candida spp, Gardnerella. vaginalis and Trichomonas. vaginalis. 24 patients with vaginitis complaints were enrolled to the study. The samples were obtained from the posterior vaginal fornix. All samples were evaluated by wet mount preparation, Gram staining, bacterial and yeast culturing and hybridization techniques. Hybridization was found to be 85.7 % sensitive and 100 % specific for Gardnerella vaginalis, and 100 % sensitive and specific for both Candida species and Trichomonas vaginalis. Since gynecologists prefer to begin the treatment immediately in most cases with vaginitis, rapid techniques such as nucleic acid hybridization technique can be employed as well as conventional methods for diagnosis. This approach will increase the success in treatment
___
- Spinillo A, Bernuzzi AM, Cevini C, Gulminetti R, Stefania Luzi, Santolo AD. The relationship of bacterial vaginosis, Candida and Trichomonas infection to symptomatic vaginitis in postmenopausal women attending a vaginitis clinic. Maturitas 1997; 27:253-260.
- Helvaci S, Gedikoglu S, Aydin Ö. Vaginal akinti örneklerinde saptanan mikroorganizmalar. Infeks Derg 1992; 2:203-205.
- Baron EJ, Finegold SM. Bailey & Scott’s Diagnostic Microbiology Genital and Sexually Transmitted Pathogens Eighth Edition, Mosby Company, St.Louis Missouuri 263-278,1990.
- Sobel JD.Vaginitis. N Engl J Med. 1997; 337:1896-1903.
- DeMeo LR, Draper DL, McGregor JA, Moore DF, Peter CR, Kapernick PS, McCormack WM. Evaluation of a deoxyribonucleic acid probe for the detection of Trichomonas vaginalis in vaginal secretions. Am J Obstet Gynecol. 1996; 174:1339-1342.
- Witt A, Petricevic L, Kaufmann U, Gregor H, Kiss H. DNA hybridization test: rapid diagnostic tool for excluding bacterial vaginosis in pregnant women with symptoms suggestive of infection. J Clin Microbiol. 2002; 40:3057-3059.
- Nugent RP, Krohn MA, Hillier SL. Relibiality of diagnosing bacterial vaginosis is improved by a standardized method of Gram stain interpretation. J Clin Microbiol 1991; 29:297-301.
- Gazi H, Degerli K, Kurt O, Teker A, Uyar Y, Caglar H, Kurutepe S, Surucuoglu S. Use of DNA hybridization test for diagnosing bacterial vaginosis in women with symptoms suggestive of infection. APMIS. 2006;114:784-787.
- Hillier SL, Gendreau RM, Dix K, Correia GS. Rapid direct detection of Candida species . Presented at ASM Conference on Candida and Candidiasis, March 25-28, 1993, Baltimore.
- Brown HL, Fuller DD, Jasper LT, Davis TE, Wright JD. Clinical evaluation of affirm VPIII in the detection and identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida species in vaginitis/vaginosis. Infect Dis Obstet Gynecol. 2004;12:17-21.
- Briselden AM, Hillier SL. Evaluation of affirm VP Microbial Identification Test for Gardnerella vaginalis and Trichomonas vaginalis. J Clin Microbiol. 1994; 32:148- 152.
- Yayın Aralığı: Yılda 3 Sayı
- Başlangıç: 1999
- Yayıncı: Düzce Üniversitesi Tıp Fakültesi