Olha PRYKHODKO, Serhii DMYTRUK, Olga AVİLOVA, Eliska KUBİKOVA, Valentyna BUMEİSTER, Olha YARMOLENKO, Olena GORDİENKO

Sıçanların Timusunun Histoultramikroskopik İncelenmesi (Deneysel Veri)

Amaç: Bu araştırma makalesi, sağlam beyaz olgun erkek laboratuvar sıçanlarının timusunun sitoarkitektonik özellikleri hakkında bilgi sunmaktadır. Çalışmanın güncelliği, her bir timus hücresi tipinin timüs yapısının oluşumuna olan katkısı hakkındaki verilerin net bir şekilde ortaya çıkarılmasına olan ihtiyaçtan kaynaklanmaktadır. Bu araştırmanın amacı, erkek olgun Wistar laboratuvar sıçanlarında timus hücrelerinin lokalizasyon ve ultramikroskopik yapısının özelliklerini belirlemektir. Gereç ve Yöntemler: Bu çalışma, 130-150 g ağırlığındaki 10 olgun erkek laboratuvar sıçanı üzerinde histolojik ve ultramikroskopik yöntemler kullanılarak yapıldı. Bir UMTP-4 (Ukrayna) mikrotomu ile yarı ince (0.5-1 μm) kesitler ve ultra ince (0.05-0.2 μm) kesitler yapıldı, yapılan bu kesitler %1 sodyum tetraborat solüsyonu ilave edilerek %1 metilen mavisi solüsyonu ile boyandı. Histolojik analiz ve fotoğraf kaydı Olympus ışık mikroskobu (Japonya) ve DSM 510 video kamera kullanılarak 1000 kez büyütme ile yapıldı. Bulgular: Timus lobüllerindeki yarı-ince kesitler ve ultra-ince kesitlerin detaylı bir şekilde incelenmesi ile timus hücrelerinin lokalizasyon ve ultramikroskobik yapısının özellikleri net bir şekilde ortaya çıkarıldı. Epitelyal, mezenkimal, vasküler ve hematopoetik timus hücrelerinin lokalizasyon ve ultramikroskopik yapısının özellikleri, bu hücrelerin fonksiyonel yükleri ve etkileşimleri açısından dikkate alınarak belirlendi. Sonuç: Timusun bileşenlerinin tanımlanmış yapısal özellikleri ve farklı bölgelerdeki göreceli konumları, simüle edilmiş biyomedikal deneylerin sonuçlarının değerlendirilmesinde gerekli olan ve istenen nesnellik düzeyinin elde edilebilmesi için dikkate alınması gereken önemli organ polimorfizmini yansıtır.

Anahtar Kelimeler:

Timus bezi, sıçanlar, histoloji, ultrastrüktür, Deney

Histoultramicroscopic Investigation of the Rats’ Thymus (Experimental Data)

Aim: The research paper presents the characteristic of cytoarchitectonics of the thymus of intact white mature male laboratory rats. Topicality of the study is due to the need to clarify the data on the contribution of each type of thymus cells in the formation of its structure. The aim of the research was to determine the specifics of localization and ultramicroscopic structure of thymus cells in male mature Wistar laboratory rats. Material and Methods: The study was conducted using histological and ultramicroscopic methods on 10 mature male laboratory rats, weighing 130-150 g. Semi-thin (0.5-1 μm) and ultrathin (0.05-0.2 μm) sections were made on a microtome UMTP-4 (Ukraine), which were stained with 1% methylene blue solution with the addition of 1% sodium tetraborate solution. Histological analysis and photographic recording were performed using Olympus light microscope (Japan) and DSM 510 camcorder with magnification in 1000 times. Results: With a detailed study of the semi-thin and ultrathin sections in the thymus lobules the specifics of localization and ultramicroscopic structure of thymus cells were clearly identified. The features of localization and ultramicroscopic structure of epithelial, mesenchymal, vascular and hematopoietic thymus cells were determined from the point of view of their functional loads and interactions. Conclusion: The described structural peculiarities of the components of the thymus and their relative location in different zones reflect significant organ polymorphism, which must be taken into account in order to achieve the required level of objectivity in the result evaluation of simulated biomedical experiments.

Keywords:

Thymus gland, Rats, histology, ultrastructure, Experiment,

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Alekseev LP, Khaitov RM. [Regulatory role of the immune system in the organism]. Ross Fiziol Zh Im I M Sechenova. 2010;96(8):787-805. Russian.
Kvetnoi IM, Yarilin AA, Polyakova VO, Knyaz'kin IV. [Neuroimmunoendocrinology of the thymus]. St. Petersburg: Dean; 2005. Russian.
Moroz GA. [Structure of intact males wistar’s rats thymus in different age]. Svit Med Biol. 2009;5(3):98-102. Russian.
Voloshyn MA, Chaikovskyi YuB, Kushch OH. [Basics of immunology and immunomorphology]. Kyev: Zaporizhzhia; 2010. Russian.
Federative International Committee on Anatomical Terminology (FICAT). Terminologia Histologica: International Terms for Human Cytology and Histology. Baltimore: Lippincott Williams & Wilkins; 2008.
Prykhodko OO, Hula VI, Yarmolenko OS, Pernakov MS, Sulim LG, Bumeister VI. Microscopic changes in rat organs under conditions of total dehydration. Azerb Med J. 2016;4:95-100.
Shyian D, Avilova O, Ladnaya I. Organometric changes of rats thymus after xenobiotics exposure. Arch Balk Med Union. 2019;54(3):422-30.
Nagakubo D, Swann JB, Birmelin S, Boehm T. Autoimmunity associated with chemically induced thymic dysplasia. Int Immunol. 2017;29(8):385-90.
Hakim FT, Memon SA, Cepeda R, Jones EC, Chow CK, Kasten-Sportes C, et al. Age-dependent incidence, time course, and consequences of thymic renewal in adults. J Clin Invest. 2005;115(4):930-9.
Parker GA. Cells of the immune system. In: Parker GA, editor. Immunopathology in toxicology and drug development. Vol 1. Immunobiology, investigative techniques, and special studies. Cham, Switzerland: Humana Press; 2017. p. 95-162.
Takahama Y, Ohigashi I, Baik S, Anderson G. Generation of diversity in thymic epithelial cells. Nat Rev Immunol. 2017;17(5):295-305.
Iwasaki A, Medzhitov R. Regulation of adaptive immunity by the innate immune system. Science. 2010;327(5963):291-5.
Jiang H, Chess L. How the immune system achieves self-nonself discrimination during adaptive immunity. Adv Immunol. 2009;102:95-133.
Beloveshkin AG. [Systemic organization of Hassall's corpuscles]. Minsk, Belarus: Medisont; 2014. Russian.
Liu D, Ellis H. The mystery of the thymus gland. Clin Anat. 2016;29(6):679-84.
Kinsella S, Dudakov JA. When the damage is done: injury and repair in thymus function. Front Immunol. 2020 Aug 12;11:1745.
Breusenko DV, Dimov ID, Klimenko ES, Karelina NR. [Modern concepts of thymus morphology]. Pediatrician (St. Petersburg). 2017;8(5):91-5. Russian.
Miller J. How the thymus shaped immunology and beyond. Immunol Cell Biol. 2019;97(3):299-304.
Lalić IM, Miljković M, Labudović-Borović M, Milić N, Milićević NM. Postnatal development of metallophilic macrophages in the rat thymus. Anat Histol Embryol. 2020;49(4):433-9.
Yuldasheva MT. [Morphological and ultramicroscopic characteristic of timus of laboratory groups of animals of prepubertatny age]. Biology and Integrative Medicine. 2017;4:12-22. Russian.
Han J, Zúñiga-Pflücker JC. A 2020 view of thymus stromal clls in T cell development. J Immunol. 2021;206(2):249-56.
Figueiredo M, Zilhão R, Neves H. Thymus inception: molecular network in the early stages of thymus organogenesis. Int J Mol Sci. 2020;21(16):5765.
Pearse G. Histopathology of the thymus. Toxicol Pathol. 2006;34(5):515-47.
Ivanovskaya TE, Zajrat'yanc OV, Leonova LV, Voloshchuk IN. [Pathology of the thymus in children]. St. Petersburg: Sotis; 1996. Russian.
Sapin MR, Nikityuk DB. [Immune system, stress and immunodeficiency]. Moscow: Dzhangar; 2000. Russian.
Elmore SA. Enhanced histopathology of the immune system: a review and update. Toxicol Pathol. 2012;40(2):148-56.
Bódi I, H-Minkó K, Prodán Z, Nagy N, Oláh I. [Structure of the thymus at the beginning of the 21th century]. Orv Hetil. 2019;160(5):163-71. Hungarian.
Caminero F, Iqbal Z, Tadi P. Histology, cytotoxic T cells. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2021.