Güven KILIÇ, Murat KAYABAŞ, Seçkin Emre CANCAN

Sıçan Subaraknoid Kanama Modelinde Bilateral Superior Servikal Ganglionektomi ve Melatonin Seviyeleri: Basit Önlemler Melatonin Düzeylerini Koruyabilir

Amaç: Subaraknoid kanama (subarachnoid hemorrhage, SAH) ciddi bir hastalıktır ve SAH sonrası melatoninin olumlu etkileri olabileceği düşünülmektedir. Superior servikal ganglionların bilateral rezeksiyonu veya blokajı melatonin seviyeleri üzerinde sabit etkilere sahiptir. Bilateral superior servikal ganglionektomili (superior cervical ganglionectomy, SCG) hayvan modelleri, superior servikal ganglionun melatonin üzerindeki rolünü göstermekte ve SAH hastalarında olumsuz sonuçların önlenmesine yardımcı olabilecek basit önlemler hakkında ipuçları vermektedir. Bu çalışmanın amacı SAH ve SCG modellerinde melatonin düzeylerinin nasıl değiştiğinin incelenmesidir. Gereç ve Yöntemler: Çalışmada 200-250 g ağırlığında kırk iki adet Sprague Dawley erkek sıçan kullanıldı ve rastgele şekilde altı gruba ayrıldı. Tüm gruplarda işlemden 24 saat sonra arteriyel kan örnekleri alındı. Grupların serum melatonin düzeyleri çalışıldı. Bulgular: SAH ve SCG grupları arasında ve kontrol grubuna karşı kan melatonin düzeylerinde anlamlı farklılık gözlendi. SCG grubu ve SAH+SCG grubu melatonin değerleri arasında anlamlı bir fark yoktu (p=0,983). SAH grubunun ortalama kan melatonin düzeyi, SCG (p<0,001), SAH+SCG (p<0.001) ve kontrol (p=0.001) gruplarından daha yüksekti. SAH+SCG ve SCG gruplarının ortalama kan melatonin düzeyleri, diğer grupların ve ayrıca SAH grubunun da (p<0,001) ortalama kan melatonin düzeylerinden daha düşüktü. Sonuç: Bilateral SCG, sıçanlarda SAH modelinden sonra serum melatonin düzeylerinin ani artışını önemli ölçüde inhibe etti. Melatoninin karmaşık sonuçlarını ele almayı amaçlayan gelecekteki çalışmalar, minör eksojen faktörlerin serum melatonin düzeylerini etkileyebileceğini hesaba katmalı ve göz önünde bulundurmalıdır.

Anahtar Kelimeler:

superior servikal ganglion, subaraknoid kanama, melatonin

Bilateral Superior Cervical Ganglionectomy and Melatonin Levels in Rat Subarachnoid Hemorrhage Model: Simple Precautions May Preserve Melatonin Levels

Aim: Subarachnoid hemorrhage (SAH) is a serious disease, and it is thought that melatonin may have positive effects after SAH. Bilateral resection or blockage of superior cervical ganglions has constant effects on melatonin levels. Animal models with bilateral superior cervical ganglionectomy (SCG) show the role of superior cervical ganglion on melatonin and give clues about simple precautions which may help to prevent unfavorable outcomes in SAH patients. The aim of this study is to examine how melatonin levels change in SAH and SCG models. Material and Methods: Forty-two Sprague Dawley male rats weighing 200-250 g were used in the study and randomly divided into six groups. Arterial blood samples were collected 24 hours after the procedure in all groups. Serum melatonin levels of the groups were studied. Results: A significant difference in blood melatonin levels was observed between SAH and SCG groups, and against the control group. There was no significant difference between the melatonin levels in SCG group and SAH+SCG group (p=0.983). The mean blood melatonin level of the SAH group was higher than the SCG (p<0.001), SAH+SCG (p<0.001) and control groups (p=0.001). The mean blood melatonin levels of SAH+SCG and SCG groups were lower than the mean blood melatonin levels of the other groups and also the SAH group (p<0.001). Conclusion: Bilateral SCG significantly inhibited the abrupt increase of serum melatonin levels after SAH model in rats. Future studies aiming to address melatonin’s complex outcomes should take into account that minor exogenous factors may affect serum melatonin levels.

Keywords:

superior cervical ganglion, subarachnoid hemorrhage, melatonin,

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