Doğal Antimikrobiyal Maddelerin Cerrahi İpliklerin Özelliklerine Etkisi

Cerrahi girişim uygulanan vücut bölgelerinde, ameliyat sonrası cerrahi alan enfeksiyonları (CAE) ortaya çıkmaktadır. Cerrahide yaralar cerrahi iplik olarak bilinen materyallerle kapatılmaktadır. Bazı cerrahi iplik türleri, CAE'ye sebep olan bakterilerin çoğalmasına neden olmaktadır. Cerrahi iplik kaynaklı enfeksiyonun önüne geçmek için antimikrobiyallerle kaplama yapılmaktadır. Bu çalışmada, CAE’nin oluşmaması için farklı tipteki cerrahi iplikler doğal antimikrobiyal maddelerle kaplanmıştır ve bu ajanların cerrahi ipliklerin morfolojik ve mekanik özelliklerine olan etkisi değerlendirilmiştir. Bu bağlamda, doğal bir polimer olan kitosan antimikrobiyal özelliğe sahip olması sebebiyle seçilmiştir ve kitosan yine doğal antimikrobiyal madde olan aloe vera ve zeytin yaprağı ekstresi ile beraber asetik asit çözeltisi içerisinde ultrason teknolojisi kullanılarak karıştırılıp kaplama solüsyonu hazırlanmıştır. Doğal maddelerin sinerjistik etkisini incelemek için farklı konsantrasyonlarda hazırlanan kaplama solüsyonlarına multifilament ipek, multifilament poliester ve monofilament poliamid cerrahi iplikler daldırılarak kaplama yapılmıştır. Fourier dönüşümlü kızılötesi spektroskopisi kullanılarak kaplanmış cerrahi ipliklerin üzerindeki fonksiyonel gruplar belirlenmiştir. Cerrahi ipliklerin yüzeyleri taramalı elektron mikroskobuyla incelenerek uzun daldırma ve kurutma işlemleri sonrası kaplama malzemesinin yüzeye güçlü bir şekilde tutunduğu gözlemlenmiştir. Ayrıca yapılan mekanik testler sonucunda kaplama işleminin cerrahi ipliklerin mekanik özelliklerini arttırdığı tespit edilmiştir.

Effect of Natural Antimicrobial Agents on the Characteristics of Surgical Sutures

Surgical site infections (SSI) occur after the surgery in body parts where the operation took place. Insurgeries, wounds are closed by thread-like materials known as sutures. Some types of sutures maypromote bacteria proliferation which is one of the leading causes of the SSI. Sutures undergo coatingprocedure to prevent infection occurrence. In this study, different types of surgical sutures werecoated with natural antimicrobial agents to evaluate their effect on morphological and mechanicalproperties of the surgical sutures. In this context, due to its antimicrobial ability, chitosan was selectedand dissolved in acetic acid solution with other natural antimicrobial agents (aloe vera and olive leafextract) through ultrasound technology. Multifilament silk, multifilament polyester, andmonofilament polyamide sutures were then dipped into those solutions prepared at differentconcentrations in order to study the synergistic effect of antimicrobial agents. Fourier transforminfrared spectroscopy with attenuated total reflectance (FTIR-ATR) was performed to identify thefunctional groups on the surface of the coated sutures. Suture surfaces were also analyzed by scanningelectron microscope (SEM) to observe the coating on the surface of sutures. Strong adhesion wasdetermined between the suture surface and the coating material after long duration of dipping anddrying procedure. It was also found that the coating process increased the mechanical properties ofthe sutures.

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