Mehmet SARİMAHMUT, Serap CELİKLER KASIMOĞULLARI

Seçilen Anadolu Bitki Türlerinin Antioksidan ve Anti-Büyüme Özellikleri: Heracleum humile, Doronicum reticulatum, Centaurea drabifolia ve Senecio olympicus'un Potansiyelini Keşfetme

Bu çalışma, Anadolu bölgesine özgü dört bitki türünün anti-büyüme ve antioksidan özelliklerini incelemiştir: Heracleum humile, Doronicum reticulatum, Centaurea drabifolia ve Senecio olympicus. Bitki materyalleri toplanmış, doğrulanmış ve bir Soxhlet aygıtı kullanılarak ekstrakte edilmiştir. Ekstrelerin büyüme inhibe edici aktiviteleri, insan meme kanseri hücre hatları MCF-7 ve MDA-MB-231 ve malignan olmayan ölümsüzleştirilmiş insan meme hücre hattı MCF-10A üzerinde sülfarodamin B (SRB) testi kullanılarak değerlendirildi. Antioksidan kapasiteler DPPH ve CUPRAC testleri ile değerlendirildi. Sonuçlar, C. drabifolia ekstresinin güçlü sitotoksik etkilere sahip olduğunu gösterirken, D. reticulatum seçici toksisite sergiledi ve değerlendirilen türler arasında en belirgin antioksidan aktiviteye sahipti. Bu bulgular, söz konusu yerli bitki türlerinin, kanser de dâhil olmak üzere çeşitli halk sağlığı sorunlarına yönelik terapötik potansiyellerini anlamamıza katkı sağlamaktadır.

Anahtar Kelimeler:

sitotoksisite, antioksidan, Heracleum humile, Doronicum reticulatum, Centaurea drabifolia, Senecio olympicus

Antioxidant and Anti-Growth Properties of Selected Anatolian Plant Species: Exploring the Potential of Heracleum humile, Doronicum reticulatum, Centaurea drabifolia, and Senecio olympicus

This study investigated the anti-growth and antioxidant properties of four plant species native to the Anatolian region: Heracleum humile, Doronicum reticulatum, Centaurea drabifolia, and Senecio olympicus. Plant materials were collected, authenticated, and extracted using a Soxhlet apparatus. The growth inhibitory activities of the extracts were evaluated in human breast cancer cell lines MCF-7 and MDA-MB-231, and the nonmalignant immortalized human breast cell line MCF-10A, using the sulforhodamine B (SRB) assay. Antioxidant capacities were assessed via DPPH and CUPRAC assays. The results demonstrated that C. drabifolia extract exhibited potent cytotoxic effects, while D. reticulatum displayed selective toxicity and the most pronounced antioxidant activity among the evaluated species. These findings contribute to our understanding of the therapeutic potential of these indigenous plant species in addressing various public health issues, including cancer.

Keywords:

cytotoxicity, antioxidant, Heracleum humile, Doronicum reticulatum, Centaurea drabifolia, Senecio olympicus,

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