Ali S. DURMUŞ, Aydın SAĞLIYAN, Eren POLAT, Cihan GÜNAY, Merve ÇELİKTEN

Tavşanlarda deneysel kornea defektlerinin iyileşmesi üzerinde sığır amniyotik sıvısının etkinliğinin klinik değerlendirmesi

Bu çalışma, amniyotik sıvının kornea epitel dokusunda oluşan defektlerdeki etkinliğini araştırmak için yapıldı. Çalışmada, deneysel olarak kornea epitelinde 3 mm çapında tam kat defekt oluşturulmuş 14 adet Yeni Zelanda tavşanı kullanıldı. Tavşanlar rastgele iki gruba ayrıldıktan sonra birinci gruptaki tavşanlara (AF) amniyon sıvısı, ikinci gruptaki tavşanlara (CG) fizyolojik tuzlu su 20 gün süreyle günde üç kez uygulandı.Çalışmanın 7., 14. ve 20. günlerinde klinik muayene ve testler yapılarak kornea defektlerinin durumu fotoğraflandı. Klinik incelemeler sonucunda AF grubundaki deneklerde gözyaşı kırılma zamanı (TBTT), floresein boyama, rose bengal boyama, korneal bulanıklık ve korneal vaskülarizasyon durumunun CG grubuna göre daha iyi olduğunu ortaya konuldu. Sonuç olarak amniyon sıvısının kornea epitel defektlerinde etkili olduğu, medikal veya operatif tedavilere ek olarak kullanılmasının faydalı olacağı sonucuna varıldı.

Anahtar Kelimeler:

amniyon, kornea, epitel, tedavi

Clinical Evaluation of the Efficacy of Bovine Amniotic Fluid on Healing of Experimental Corneal Defects in Rabbits

This study was conducted to investigate the effectiveness of amniotic fluid in defects in the epithelial tissue of the cornea. In the study, 14 New Zealand rabbits, which were experimentally created with a 3 mm diameter full-thickness defect in the corneal epithelium, were used. After the rabbits were randomly divided into two groups, amniotic fluid was applied to the rabbits in the first group (AF) and saline solution was applied to the rabbits in the second group (CG) three times a day for 20 days. Clinical examinations and tests were performed on the 7th, 14th, and 20th days of the study and the condition of the corneal defects was photographed. Clinical examinations revealed that tear break-up time (TBTT), fluorescein staining, rose bengal staining, corneal turbidity, and corneal vascularization were better in the AF group than in the CG group. As a result, it is concluded that amniotic fluid is effective in corneal epithelial defects and it would be beneficial to use it in addition to medical or operative treatment.

Keywords:

amnion, cornea, epithel, treatment,

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