Muhammet Lütfi SELCUK, Hasan Hüseyin DONMEZ, Fatma COLAKOGLU

Metimazolün Uterus Katman Kalınlıklarına ve Eozinofil Granülosit Değişimlerine Etkisi

Bu çalışmanın amacı ratlarda hipotiroidizm oluşturmada kullanılan metimazolün uterus katman kalınlıklarına ve eozinofil granülositler üzerindeki etkilerini değerlendirmektedir. Çalışmanın uterus örnekleri kontrol (K, n=6) ve deney (D, n=9) gruplarını oluşturan 12-14 haftalık 15 sağlıklı dişi Wistar Albino rattan alındı. Deney grubuna 2 hafta boyunca intraperitoneyal olarak 10 mg/kg/gün dozunda metimazol enjekte edildi. Deney grubunda hipotiroidizm oluşturulduktan sonra doku üzerindeki kronik etkileri görmek için çalışmadaki tüm ratların beslenmesine 2 hafta daha normal pelet yemle devam edildi. 4. haftanın sonunda alınan uterus örnekleri Crossmon’s trikrom boyamasıyla boyandı. Endometriyum, miyometriyum ve perimetriyum katmanlarını içeren 6 farklı uterus görüntüsünün uterus katman kalınlıkları ImageJ analiz programı kullanılarak ölçüldü. Endometriyumdaki ortalama eozinofil granülosit sayısı kontrol grubuna kıyasla deney grubunda daha yüksekti (P

Effect of Methimazole on Eosinophil Granulocyte Changes and Uterus Layer Thicknesses

The purpose of this research was to evaluate the effects of methimazole used to create hypothyroidism in rats on the eosinophilic cellsand uterus layer thicknesses. Uterus samples, 15 healthy Wistar Albino female rats aged 12-14 weeks were taken from control (C, n=6)and experimental (E, n=9) groups. Intraperitoneally, methimazole was injected as 10 mg/kg/day dose in the Group E for 2 weeks. Afterhypothyroidism was created in the Group E, feeding all the rats in this study was continued with normal pellet feed again for 2 weeks tosee chronic effects on tissue. At the end of the 4th week, uterus samples were stained with Crossmon’s trichrome staining. Uterus layerthicknesses were measuring with ImageJ Analysis using images of six different uterus regions including endometrium, myometrium andperimetrium layers. The mean number of eosinophil granulocyte in the endometrium was higher in Group E when compared to Group C(P0.05). We concluded that methimazole might cause hypereosinophil granulocyte accumulation inthe endometrium functionalis. This study is important for clinicians to evaluate methimazole-associated eosinophilic infiltration due tohypersensitive immune reactions to the drugs, especially used during pregnancy.

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