İneklerde Subklinik Mastitis Tanısında Amiloid A ve C-Reaktif Protein Düzeylerinin Değerlendirilmesi

Subklinik mastitis, ineklerde en yaygın gözlenen önemli yangısal sürü hastalıklarından birisidir. Bu çalışmada hastalığın tan ısında süt ve kanda amiloid A (AA) ve C-reaktif protein (CRP) parametrelerinin yeni bir tanı seçeneği olarak ortaya konulması amaçlanmıştır. Çalışma laktasyonun farklı dönemlerinde bulunan toplam 40 adet Holstein ırkı sağmal inek üzerinde yürütüldü. Strip cup muayenesinin a rdından süt ve meme muayenesinde herhangi bir fiziksel bozukluk gözlenmeyen meme loblarına subklinik mastitis (SCM) tanısı amacıyla Kaliforniya Mastitis Testi (CMT) uygulandı. CMT’de sadece Ş ve +1 skorlar SCM pozitif olarak çalışmaya dahil edildi. Bu sonuçlar doğrultusunda 2 grup oluşturuldu; Grup I (n= 40), CMT yönünden her 4 meme lobu da negatif olan ineklere ait serum ve süt örnekleri, Grup II (n= 40)’ye ise CMT’ye göre pozitif olan serum ve süt örnekleri dahil edildi. Süt ve kan serumunda amiloid A ve CRP düzeyinin belirlenmesinde ticari ELISA kitleri kullanıldı. Çalışmada, ortalama AA düzeyleri sağlıklı olan serum ve süt örneklerinde sırasıyla 0.68±0.00 ng/ml; 0.66±0.00 ng/ml; SCM olanlarda ise sırasıyla 2.04±0.06 ng/ml ve 1.84±0.06 ng/ml belirlendi (p

Evaluation of Amyloid A and C-Reactive Protein Levels in The Diagnosis of Subclinic Mastitis in Cows

Subclinical mastitis is one of the most common inflammatory dairy herd diseases in cows. In this study, we aimed to present amyloid A (AA) and C-reactive protein (CRP) parameters in milk and blood as a new diagnostic option in the diagnosis of subclinical mastitis. The study was carried out on a total of 40 Holstein cows in different stages of lactation. After the strip cup examination, the California Mastitis Test (CMT) was applied for the diagnosis of subclinical mastitis (SCM).to the mammary lobes in which no physical symptom was observed in the milk and udder examination. Only S and +1 scores in CMT were included in the study as SCM positive. In line with these results, 2 groups were formed; serum and milk samples from cows with negative CMT in all 4 mammary lobes were included in Group I (n= 40), and serum and milk samples positive for CMT in Group II (n= 40) were included. In the study, mean AA levels were 0.68± 0.00 ng/ml; 0.66± 0.00 ng/ml in healthy serum and milk samples, respectively. In those with SCM, 2.04± 0.06 ng/ml and 1.84± 0.06 ng/ml were determined, respectively (p< 0.001). CRP levels were 0.17± 0.00 mg/ml; 0.15± 0.00 mg/ml in serum and milk in healthy group, respectively. It was calculated as 0.55± 0.01 mg/ml and 0.53± 0.01 mg/ml in SCM, respectively (p< 0.001). In the evaluation of acute phase protein parameters between groups; It was determined that AA and CRP milk and blood serum values were statistically significantly different between the groups. As a result, it is thought that AA and CRP may be prominent in determining subclinical mastitis.

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